31 research outputs found

    Graphene Surface-Anchored Fluorescence Sensor for Sensitive Detection of MicroRNA Coupled with Enzyme-Free Signal Amplification of Hybridization Chain Reaction

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    A new enzyme-free signal amplification-based assay for microRNA (miRNA) detection is developed by using hybridization chain reaction (HCR) coupled with a graphene oxide (GO) surface-anchored fluorescence signal readout pathway. MiRNAs can efficiently initiate HCR between two species of fluorescent hairpin probes. After HCR, both of the excess hairpin probes and the HCR products will be anchored on the GO surface. The fluorescence of the hairpin probes can be completely quenched by GO, whereas the HCR products maintain strong fluorescence. Therefore, integrating HCR strategy for signal amplification with selective fluorescence quenching effects of GO provides a versatile miRNA assay

    Sex ratio, female weight and longevity (mean ┬▒ SE) of <i>Nilaparvata lugens</i> developed on rice germplasms.

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    <p>A: Sex ratio (n = 3). B: Female weight (n = 30). C: Female longevity (n = 15). The different letters over the bars in a panel indicate significant differences (Tukey HSD, <i>P</i> = 0.05).</p

    Developmental duration (A, n = 240) and survival (B, n = 3) (mean ┬▒ SE) of <i>Nilaparvata lugens</i> nymphs on rice germplasms.

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    <p>Different letters over the bars in a panel indicate significant differences (Tukey HSD, <i>P</i> = 0.05).</p

    Population size (no./100 hills, mean ┬▒ SE) of <i>Nilaparvata lugens</i> in field plots (n = 3) of the TN1, IR64, B5, HHZ, BR4831, 9311, HF106, and C602 rice germplasms.

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    <p>Population size (no./100 hills, mean ┬▒ SE) of <i>Nilaparvata lugens</i> in field plots (n = 3) of the TN1, IR64, B5, HHZ, BR4831, 9311, HF106, and C602 rice germplasms.</p

    Population parameters (mean ┬▒ SE) of <i>Nilaparvata lugens</i> fed on the TN1, IR64, B5, HHZ, BR4831, 9311, HF106, and C602 rice germplasms.

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    <p>Population parameters (mean ┬▒ SE) of <i>Nilaparvata lugens</i> fed on the TN1, IR64, B5, HHZ, BR4831, 9311, HF106, and C602 rice germplasms.</p

    Weight of honeydew (mean ┬▒ SE, n = 30) excreted by female <i>Nilaparvata lugens</i> adults on rice germplasms.

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    <p>Different letters over the bars indicate significant differences (Tukey HSD, <i>P</i> = 0.05).</p

    Typical EPG waveforms identified from <i>Sogatella furcifera</i> on rice and corn plants.

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    <p>np: non-penetration, N1: penetration initiation, N2: salivation and stylet movement, N3: extracellular stylet activity near the phloem region, N4-a: intracellular stylet activity in the phloem region without ingestion, N4-b: phloem sap ingestion, N5: stylets in the xylem tissue.</p
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