Additional file 5: of Innate immune activation of astrocytes impairs neurodevelopment via upregulation of follistatin-like 1 and interferon-induced transmembrane protein 3

Figure S5. Expression of Fstl1 in polyI:C-treated Ifitm3 KO mice, related to Fig. 5. Hippocampal brain slices prepared from vehicle- or polyI:C-treated Ifitm3 KO mice were immunostained with indicated antibodies. Scale bar, 20 μm. (PPTX 23832 kb

Additional file 6: of Innate immune activation of astrocytes impairs neurodevelopment via upregulation of follistatin-like 1 and interferon-induced transmembrane protein 3

Figure S6. Combinatory treatment of rBmp4 with rFstl1, related to Fig. 4. Neurons were cultured for 5 days (DIV2-7) with culture medium supplemented with the indicated concentration of rmFstl1 and/or rBmp-4. MAP2-positive dendrite length of neurons was measured. Values indicate the means ± SE of three independent experiments. Scale bar, 50 μm. (PPTX 120 kb

Additional file 2: of Innate immune activation of astrocytes impairs neurodevelopment via upregulation of follistatin-like 1 and interferon-induced transmembrane protein 3

Figure S2. Effect of rFstl1 treatment on neurite branch, related to Fig. 4. Neurons were treated with indicated concentration of rmFstl1 or vehicle. Branched number of neurites was counted. Values indicate the means ± SE (n = 26–36). (PPTX 46 kb

Additional file 1: of Innate immune activation of astrocytes impairs neurodevelopment via upregulation of follistatin-like 1 and interferon-induced transmembrane protein 3

Figure S1. Validation of Fslt1 knockdown, related to Fig. 3. Culture astrocytes were transfected with siRNA targeting for Fstl1 or control siRNA. Western blotting was performed with indicated antibodies. (PPTX 66 kb

Additional file 3: of Innate immune activation of astrocytes impairs neurodevelopment via upregulation of follistatin-like 1 and interferon-induced transmembrane protein 3

Figure S3. Effect of rFstl1 treatment on cell viability, related to Fig. 4. Neurons were treated with indicated concentration of rmFstl1 or vehicle. The cell viability of neurons was measured. Values indicate the means ± SE (n = 3). (PPTX 43 kb

Additional file 4: of Innate immune activation of astrocytes impairs neurodevelopment via upregulation of follistatin-like 1 and interferon-induced transmembrane protein 3

Figure S4. Co-expression of Fstl1 with Ifitm3 in the hippocampus of polyI:C-treated neonatal mice, related to Fig. 5. Hippocampal sections prepared from mice treated with vehicle or polyI:C were immunostained with indicated antibodies. Scale bar, 20 μm. (PPTX 470 kb

sj-pdf-1-vmj-10.1177_1358863X231225463 – Supplemental material for Risk factors for recurrent ischemic events in symptomatic carotid artery stenosis on CT angiography

Supplemental material, sj-pdf-1-vmj-10.1177_1358863X231225463 for Risk factors for recurrent ischemic events in symptomatic carotid artery stenosis on CT angiography by Takeyoshi Tsutsui, Kiyofumi Yamada, Taichi Ikedo, Yoshiaki Morita, Eika Hamano, Hirotoshi Imamura, Hisae Mori, Koji Iihara and Hiroharu Kataoka in Vascular Medicine</p

Optimization of inversion slab thickness for SNAP imaging.

<p>It is clear that the SNAP MRA performance is best when the inversion pulse was non-selective (NS) compared to the inversion slab thicknesses considered. This implementation was used for the subsequent validation scans.</p

Visual comparison between SNAP and TOF MRA on the same subjects.

<p>SNAP and TOF make same detection on stenotic lesions on both subjects (dotted circles). The small artery visualization on SNAP MRA is significantly improved (arrows).</p

Definition of MCA segments for SVB comparison.

<p>The orange dots identify the bifurcations and the dotted lines delineate the different segments (M1-M4) of the MCA artery. Note that the oblique projection direction is used to better visualize the different segments.</p