population information

This file contains information on the 27 populations. %F denotes the percentage of female plants (opposed to hermaphroditic ones) in the population. Pop. size is the populations size expressed in the number of individual plants. Age is the age of the population. n=new (founder): o=old (established)

Assignment of individuals of recent populations (columns) to old populations (rows).

<p>Assignment of individuals of recent populations (columns) to old populations (rows).</p

GenAlEx input file

This file contains the raw microsatellite (SSR) dataset in GenAlEx format. This file can be converted to other formats using GenAlEx. SSR data for 27 diploid populations of Origanum vulgare, each consisting of 20 individual plants characterized by 10 SSR loci

Isolation effect on genetic assignment.

<p>Correlation between the number of individuals of recent populations assigned to a source (old) population and the distance of the recent population to this source population (<i>β</i> = –0.25, <i>P</i> = 0.018). Data points represent assignment data from all 14 recent populations (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067255#pone-0067255-t004" target="_blank">Table 4</a>).</p

Parameter estimates of performed bootstrapping analysis on pairwise differentiation for recent and old populations and differences between recent and old populations tested based on bootstrap t-tests.

<p>All tests are based on 9999 bootstraps. CI: 95% confidence intervals. Significance: ** 0.01≥ <i>P</i>-value > 0.001 *** 0.001≥ <i>P</i>-value.</p

Study area in the Viroin valley.

<p>Figure visualises the sampled recent populations (grey) and old populations (black) of <i>O. vulgare</i>. Ancient (white) and restored (dotted) calcareous grasslands are visualised as well. Population codes correspond to those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067255#pone-0067255-t001" target="_blank">table 1</a>.</p

SNP sequences and GENALEX file

Sequences of the 250 SNPs (together with their functional annotations) are available in tab 1 of the file. In tab 2, the original GENALEX file on the 183 successfully genotyped SNPs is provided

Map of the sampled recent populations (italics) and old populations (bold) of <i>Origanum vulgare</i>.

<p>Pie slices correspond to population membership to the four genetic groups defined by the Bayesian assignment analysis of Structure <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067255#pone.0067255-Pritchard1" target="_blank">[55]</a>. Group 1: blue, group 2: red, group 3: green, group 4: purple. Population codes correspond to those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067255#pone-0067255-t001" target="_blank">table 1</a>.</p

Difference in observed heterozygosity and inbreeding coefficient between recent and old populations.

<p>A. Boxplot for observed heterozygosity (H_O). B. Boxplot for inbreeding coefficient (F_IS).</p

Relation between the mean number of alleles (A) and the percentage of female plants (%F).

<p>The relation is visualized independent for small (<500 plants, open symbol, no regression line shown) (<i>r</i> = –0.29, <i>P</i> = 0.29) and large populations (>1000 plants, full symbol, continuous line) (<i>r</i> = 0.64, <i>P</i> = 0.048). Recent populations are presented as diamonds, old populations as circles. %F was log transformed.</p