2 research outputs found

    Additional file 1 of Functionalizing silica sol–gel with entrapped plant virus-based immunosorbent nanoparticles

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    Additional file 1: Figure S1. Bead area over time (Cy5-TMV, 2 µL bead). Normalized bead area over time for 2 µL volume silica bead containing Cy5-TMV over 48 h. Beads were exchanged into fresh PBS buffer after each measurement. Error bars represent one standard deviation with biological triplicate

    Additional file 2 of Functionalizing silica sol–gel with entrapped plant virus-based immunosorbent nanoparticles

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    Additional file 2: Figure S2. Absorbance readings during equilibration (VIN and wt-TMV, 40 µL bead). UV–vis A280 measurements of the PBS wash solution over ~ 2 days of equilibration reported as a fraction of the initial A280 measurement for the VIN or wt-TMV added into the silica sol–gel synthesis. The PBS wash solution was collected at each sample timepoint and exchanged with fresh buffer. This experiment was conducted using 1 volume PBS wash solution per volume silica sol–gel to improve limit of detection. The retention of the PBS wash solution in the buffer exchange (~ 50% total wash solution) dictates that these results represent an upper bound of the initial mass lost during washing. Error bars represent one standard deviation using biological triplicat
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