Additional file 1 of CircRbms1 knockdown alleviates hypoxia-induced cardiomyocyte injury via regulating the miR-742-3p/FOXO1 axis

Additional file 1: Fig. S1. Effects of circRbms1 knockdown on cell cycle process in hypoxia-induced H9c2 cells. H9c2 cells were transfected with or without si-NC (50 nM), si-circRbms1 (50 nM), vector (4.0 µg), or circRbms1 (4.0 µg), and then treated with hypoxia. Untreated H9c2 cells were used as control. A, B Flow cytometry was used to assess the cell cycle distribution. All experiments were repeated three times. ***P < 0.00

CO regulation of LR primordia initiation and LR emergence over the time

Seedlings were grown hydroponically for 2 d after germination and then treated with 0 and 10 μM CO for the indicated time. (a) Time-course of LR primordia initiation with or without CO exposure. (b) Time-course of lateral root emergence with or without CO exposure. Values represent the mean of three independent experiments and vertical bars indicate standard deviations (=45 seedlings). Asterisks indicate that the mean values are significantly different between the CO treatments and controls ( <p><b>Copyright information:</b></p><p>Taken from "Regulation of tomato lateral root development by carbon monoxide and involvement in auxin and nitric oxide"</p><p></p><p>Journal of Experimental Botany 2008;59(12):3443-3452.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2529230.</p><p></p

Visualization of NO generation in tomato roots exposed to carbon monoxide

(a) Visualization of NO in primary roots. Seedlings were treated with CO at 0, 10, and 50 μM for 24 h. After treatments, the seedling roots were loaded with 15 μM 4,5-diaminofluorescence (DAF-2DA) for 15 min and immediately photographed (bar = 5 mm). (b) Visualization of NO in lateral roots. Seedlings were treated with 0 and 10 μM CO for 12, 24, 36, 48, and 60 h and then exposed to DAF-2DA for 15 min. After that, they were immediately photographed (bar = 10 mm).<p><b>Copyright information:</b></p><p>Taken from "Regulation of tomato lateral root development by carbon monoxide and involvement in auxin and nitric oxide"</p><p></p><p>Journal of Experimental Botany 2008;59(12):3443-3452.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2529230.</p><p></p

The amount of transcripts and proteins of tomato root LeHO-1 during the development of lateral roots exposed to CO

Tomato seedlings were grown hydroponically for 2 d after germination and then treated with 10 μM CO for 0, 24, 48, 72, and 96 h. (a) Analysis of transcripts by semi-quantitative RT-PCR. was used for cDNA normalization. The number below the band indicates relative abundance (RA) of with respect to the loading control actin. (b) Immunoblot analysis. Extracts from tomato roots were analysed by protein gel blotting using an antibody raised against LeHO-1 proteins. The molecular mass of the proteins is indicated on the right in kilodaltons. The results shown above were from one of the three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Regulation of tomato lateral root development by carbon monoxide and involvement in auxin and nitric oxide"</p><p></p><p>Journal of Experimental Botany 2008;59(12):3443-3452.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2529230.</p><p></p

CO regulation of IAA accumulation and distribution in tomato root, leaf, and stem during lateral root development

Seedlings were grown hydroponically for 2 d after germination and then treated with the indicated concentrations of CO for 12 h. After that, the tissues were sampled and IAA was measured by HPLC. Values represent the mean of two independent experiments and vertical bars indicate the standard deviations (=90 seedlings). Asterisks indicate that the mean values are significantly different between the CO treatments and controls ( <p><b>Copyright information:</b></p><p>Taken from "Regulation of tomato lateral root development by carbon monoxide and involvement in auxin and nitric oxide"</p><p></p><p>Journal of Experimental Botany 2008;59(12):3443-3452.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2529230.</p><p></p

Exploring Catalyst and Solvent Effects in the Multicomponent Synthesis of Pyridine-3,5-dicarbonitriles

The effects of an ionic base, tetrabutylammonium hydroxide (TBAH), and an amine base, piperidine, on the direct synthesis of pyridine-3,5-dicarbonitriles using a multicomponent reaction (MCR) from aldehydes, malononitrile, and thiols were systematically investigated. The amine base showed better results when the MCR was performed in ethanol, whereas employing the ionic base in acetonitrile resulted in similar yields but in a much shorter reaction time. A modified protocol to overcome the difficulty in the direct synthesis of pyridine-3,5-dicarbonitriles via the MCR from sterically hindered aldehydes using either base was realized by changing the reaction solvent from ethanol to acetonitrile. Mechanistically, the two catalysts were found to each promote different pathways in the final oxidation step of the penultimate product, 1,4-dihydropyridine 6. A reaction intermediate, Knoevenagel adduct 7, plays the major role in the amine base-catalyzed system, while in the presence of an ionic base, aerobic oxygen acts as the primary oxidant

CO regulation of the lateral root development of tomato (a, b, c), Arabidopsis (ecotypes, Landsberg ) (d), and rapeseed () (e)

Tomato seedlings were grown hydroponically for 2 d after germination and then treated with the indicated concentrations of CO for 4 d. (a) Photograph of tomato lateral root formation. (b) The number of tomato lateral roots (LR) exposed to different concentrations of CO. (c) Change of elongation of tomato primary root (PR) with different levels of CO. Values represent the mean of three independent experiments and vertical bars indicate standard deviations (=45 seedlings). Asterisks indicate that the mean values are significantly different between the CO treatments and controls ( <p><b>Copyright information:</b></p><p>Taken from "Regulation of tomato lateral root development by carbon monoxide and involvement in auxin and nitric oxide"</p><p></p><p>Journal of Experimental Botany 2008;59(12):3443-3452.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2529230.</p><p></p

Effect of 1-naphthalene acetic acid (NAA) on the LR emergence of tomato mutant (LA2469A)

Seedlings were grown hydroponically for 2 d after germination and then treated with the indicated concentrations of NAA for 5 d. (a) The number of lateral roots (LR) exposed to the indicated concentrations of NAA. (b) Photograph of lateral root emergence. (c) Change of elongation of the primary root (PR). (d) Hypocotyl elongation. Values represent the mean of three independent experiments and vertical bars indicate standard deviations (=45 seedlings). Asterisks indicate that the mean values are significantly different between the NAA treatments and controls ( <p><b>Copyright information:</b></p><p>Taken from "Regulation of tomato lateral root development by carbon monoxide and involvement in auxin and nitric oxide"</p><p></p><p>Journal of Experimental Botany 2008;59(12):3443-3452.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2529230.</p><p></p

sj-docx-1-pie-10.1177_09544089231172328 - Supplemental material for Changes in the mode of transportation using active magnetic technology for non-standard equipment

Supplemental material, sj-docx-1-pie-10.1177_09544089231172328 for Changes in the mode of transportation using active magnetic technology for non-standard equipment by Hongsheng Zhang, Yanbin Li and Kai Guo in Proceedings of the Institution of Mechanical Engineers, Part E: Journal of Process Mechanical Engineering</p