Semi-quantitative rt-PCR analysis showing total ET-1 mRNA expression in the retinae of non-transgenic (NTg), heterozygous (He) and homozygous (Hm) TET-1 mice.

<p>A: Ethidium bromide-stained agarose gels of rt-PCR products of ET-1, showing increased ET-1 mRNA expression in transgenic mouse retina. The molecular size marker (M) was 1 kb plus marker. B: Histograms showing the levels of ET-1 mRNA expression normalized to that of GAPDH. n = 4 for each experimental group. *: <i>p</i><0.05 (Kruskal-Wallis Test).</p

Thicknesses of inner nuclear layer (INL), inner plexiform layer (IPL) to inner limiting membrane (ILM) and the whole inner retina (µm).

<p>Thicknesses of inner nuclear layer (INL), inner plexiform layer (IPL) to inner limiting membrane (ILM) and the whole inner retina (µm).</p

Severity of the presence of pyknotic nuclei in cells in ganglion cell layer (GCL) after transient inner retinal ischemia.

<p>Severity of the presence of pyknotic nuclei in cells in ganglion cell layer (GCL) after transient inner retinal ischemia.</p

Immunohistochemical staining of aquaporin-4 (AQP4).

<p>Up-regulation of AQP4 signal was found in the Müller cell processes in IPL (arrows) and Müller cell bodies in INL (arrowheads) in the ipsilateral retina of Hm TET-1 mice compared with the corresponding contralateral retina, while similar intensity of AQP4 signal was present in the contralateral and ipsilateral retinae of NTg mice. n = 5 for each experimental group. Scale bar = 50 µm.</p

Immunohistochemical staining of glial fibrillary acidic protein (GFAP).

<p>Compared with the contralateral retinae of NTg and Hm TET-1 mice, GFAP signal was up-regulated in the ipsilateral retinae in the astrocytes around capillaries in the inner limiting membrane (arrowheads) and the Müller cell processes in IPL (arrows). n = 5 for each experimental group. Scale bar = 50 µm.</p