Capsella_alignments.tar

Used alignements for the empirical application from Capsella. The sequences came from St Onge KR, Foxe JP, Li J et al. (2012, Molecular biology and evolution), Slotte T, Huang H, Lascoux M, Ceplitis A (2008, Molecular biology and evolution) and Foxe JP, Slotte T, Stahl EA et al. (2009, Proceedings of the National Academy of Sciences of the United States of America)

Total number of leaves (a) and above-ground biomass (b) of male and hermaphrodite plants of <i>M. annua</i> growing with and without herbivores.

<p>Values are means ± SE (N = 34).</p

Percentage of total plant damage (a), number of damaged leaves by plant (b) and percentage of leaf damage to damaged leaves (c) in males (“male”) and hermaphrodites (“herm”) of <i>M. annua</i> after exposure to snail's herbivory.

<p>Values are means ± SE (N = 39).</p

Locations of each of the 10 sites used in this study.

<p>The arrow indicates the location of the study region in Spain. Grid lines indicate (decimal) degrees north and east.</p

Variation in the frequency of females (grey bar segments), hermaphrodites (white bar segments), and males (black bar segments) at each high density (left panel) and low density (right panel) subsite.

<p>Subsites in each panel are ordered by the local frequency of females in the high-density subsites. Numbers at the top of each bar indicate the total sample size per subsite. Numbers at the bottom of each bar correspond with the site numbers in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035597#pone-0035597-g001" target="_blank">Fig. 1</a>.</p

Differences in the proportion of above-ground biomass allocated to seeds between females (<i>σ_f</i> - diamonds) and hermaphrodites (<i>σ_h</i> - triangles) in plots with and without males.

<p>Means (± S.E.) of plants grown under high densities are joined with a solid line, and with a dashed line for plants grown under low densities.</p

Differences in the proportion of above-ground biomass allocated to pollen between males (<i>π_m</i> - circles) and hermaphrodites (<i>π_h</i> - triangles) in high and low density treatments.

<p>Error bars represent one standard error.</p

Linear mixed-effects models used to evaluate patterns of allocation to seed production by females (<i>σ</i>_f) and hermaphrodites (<i>σ</i>_h) and the allocation to pollen production by males (<i>π</i>_m) and hermaphrodites (<i>π</i>_h).

*<p><i>P</i><0.05;</p>**<p><i>P</i><0.001;</p>***<p><i>P</i><0.0001.</p

Figure 5

<p>Parental and progeny sex ratios of females (squares), hermaphrodites (triangles), and males (circles) in high density treatments with males (A), without males (C), and low density treatments with males (B) and without males (D) (a small proportion of males were found in the low density with no males treatment, but this is not depicted). Results of the replicated goodness of fit tests from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035597#pone-0035597-t002" target="_blank">Table 2</a> (pooled for each density by male treatment combination) are shown in each panel (* <i>P</i><0.0001; ^NS<i>P</i>>0.05).</p

Frequency of males, hermaphrodites, and females among the progeny of plants grown under high- vs. low-density conditions and in the presence vs. absence of males.

<p>Shown are the relative pollen production of hermaphrodites versus males (<i>p</i>_h, where <i>p_h</i> = π_h/π_m), the relative seed production of hermaphrodites versus females (<i>o_h</i>, where <i>o_h</i> = σ_h/σ_f), the frequency of each sex in the next generation, and the sample size. <i>G</i> values are given for tests of independence of sex frequencies from the parental versus progeny generation for each plot. <i>G</i>_H refers to tests of heterogeneity between plots in the same treatment. <i>G</i>_P combines data from all plots, testing for overall changes in the frequency of the three sex phenotypes.</p>*<p><i>P</i><0.05;</p>**<p><i>P</i><0.01;</p>***<p><i>P</i><0.001.</p