Additional file 5: Table S4. of Variations on a theme: Genomics of sex determination in the cichlid fish Astatotilapia burtoni

Loci with male-specific coverage and higher coverage in daughters of the A. burtoni Chipwa wild-caught cross with corresponding location in BLAST on O. niloticus genome. (XLSX 83 kb

Additional file 2: Table S1. of Variations on a theme: Genomics of sex determination in the cichlid fish Astatotilapia burtoni

Loci with male-specific coverage and higher coverage in daughters of the A. burtoni lab strain cross with corresponding location in BLAST on O. niloticus genome and PCR conditions and results for male specific loci. (XLSX 1227 kb

Additional file 1: Figure S1. of Variations on a theme: Genomics of sex determination in the cichlid fish Astatotilapia burtoni

Sampling location of the “Chipwa wild-caught” fish parents used in this study. (PNG 2140 kb

Additional file 7: Figure S2. of Variations on a theme: Genomics of sex determination in the cichlid fish Astatotilapia burtoni

Gene expression of A. burtoni lab strain along the reference chromosomes of the Nile tilapia. Gene expression in male and female gonads of the A. burtoni lab strain along the reference chromosomes of the Nile tilapia as in Fig. 3c. (ZIP 329 kb

Additional file 4: Table S3. of Variations on a theme: Genomics of sex determination in the cichlid fish Astatotilapia burtoni

RAD loci differentiating males and females of the A. burtoni Chipwa wild-caught fish with corresponding BLAST results on the O. niloticus genome. SNPs in bold show inheritance of alleles from father to sons only, supporting an XX-XY system. (XLS 807 kb

Additional file 3: Table S2. of Variations on a theme: Genomics of sex determination in the cichlid fish Astatotilapia burtoni

RAD SNPs differentiating males and females of the A. burtoni lab strain with corresponding BLAST results on the O. niloticus and A. burtoni genome. SNPs in bold show inheritance of alleles from father to sons only, supporting an XX-XY system. (XLS 56 kb

Measurement of bacterial cytosol buffering capacity

Bed file of Xiphophorus piRNA clusters. (BED 1072 kb

Additional file 9: Figure S6. of Germ cell and tumor associated piRNAs in the medaka and Xiphophorus melanoma models

Histogram of bases within piRNA cluster 3. Red rectangles indicate regions selected for primer design. (PDF 42 kb

Additional file 12: Table S6. of Germ cell and tumor associated piRNAs in the medaka and Xiphophorus melanoma models

Bed file of Xiphophorus piRNA clusters. (BED 1072 kb

Additional file 6: Table S3. of Germ cell and tumor associated piRNAs in the medaka and Xiphophorus melanoma models

Target genes of four selected piRNAs as predicted by RNAhybrid. Thresholds for target selection were minimum free energy < −30 AND p-value < 0.05. In the 4th column log fold changes of the genes in the corresponding mRNA dataset are given. (XLSX 14 kb