Cr-Doped ZnO Nanoparticles: Synthesis, Characterization, Adsorption Property, and Recyclability

In this paper, a mild solvothermal method has been employed to successfully synthesize a series of Cr-doped ZnO nanoparticles (NPs) with different Cr³⁺ contents, which is a kind of novel and high-efficiency absorbent for the removal of acid dye methyl orange (MO) from aqueous solution. The as-prepared products were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), Brunauer, Emmet, and Teller (BET), and Zeta potential measurements. In accordance with the adsorption capacity of the products, the obtained optimal Cr/Zn molar ratio is 6%. The adsorption process of MO on Cr-doped ZnO was investigated by kinetics, thermodynamics, and isotherm technologies, which, respectively, indicated that the adsorption was fast (adsorption reached equilibrium in 2 h) and followed a pseudo-second-order model, that the adsorption process was spontaneous and endothermic, and that it agreed well with the Langmuir isotherm with a maximum adsorption capacity of 310.56 mg g^–1. Moreover, a reasonable mechanism was proposed to elucidate the reasons for their adsorption behavior. In addition, a simple and low-cost chemical method was developed to separate and recycle ZnO and MO from the used adsorbent, effectively avoiding the secondary pollution. This work can not only describe efficient experimental approaches for obtaining novel adsorbents and recycling them but also offer valuable clues for the preparation and property study of other semiconductor adsorbents

Lower vitamin D levels and VDR variants are risk factors for breast cancer: an updated meta-analysis

Inadequate vitamin D levels and vitamin D variants have been shown to be associated with breast cancer (BC), however the results are inconsistent. To reach a definitive conclusion the present meta-analysis was conducted. When compared to healthy controls, BC patients had reduced vitamin D levels (standard difference in means = −0.564, p = 0.003). The meta-analysis revealed that the FokI mutation was linked with an increased BC susceptibility (CC vs. TT: OR = 1.107, p = 0.001, CC vs. TC + TT: OR = 1.114, p = 0.020). There was no role of other VDR variants (BsmI, TaqI, and ApaI). FokI mutation and diminished vitamin D increase the likelihood of developing BC.</p

Additional file 6 of NPM3 as a novel oncogenic factor and poor prognostic marker contributes to cell proliferation and migration in lung adenocarcinoma

Additional file 6: Supplementary Table S3. The genes positively associated with NPM3 expression

Additional file 5 of NPM3 as a novel oncogenic factor and poor prognostic marker contributes to cell proliferation and migration in lung adenocarcinoma

Additional file 5: Supplementary Table S2. A protein-protein interactionnetwork parameter based on NPM3

Data_Sheet_1_A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder.docx

Vibrio anguillarum is a pathogenic bacterium that infects flounder resulting in significant losses in the aquaculture industry. The VAA protein previously identified in flounder is associated with a role in immune protection within these fish. In the present study, a recombinant DNA plasmid encoding the VAA gene of V. anguillarum was constructed and its potential as a DNA vaccine, to prevent the infection of V. anguillarum in flounder fish, investigated. We verified the expression of the VAA protein both in vitro in cell lines and in vivo in flounder fish. The protective effects of pcDNA3.1-VAA (pVAA) were analyzed by determination of the percentage of sIgM+, CD4-1+, CD4-2+, CD8β+ lymphocytes, and the production of VAA-specific antibodies in flounder following their immunization with the DNA vaccine. Histopathological changes in immune related tissues, bacterial load, and relative percentage survival rates of flounder post-challenge with V. anguillarum, were all investigated to assess the efficacy of the pVAA DNA vaccine candidate. Fish intramuscularly immunized with pVAA showed a significant increase in CD4-1+, CD4-2+, and CD8β+ T lymphocytes at days 9, 11, and 14 post-vaccination, reaching peak T-cell levels at days 11 or 14 post-immunization. The percentage of sIgM+ lymphocytes reached peak levels at weeks 4–5 post-immunization. Specific anti-V. anguillarum or anti-rVAA antibodies were induced in inoculated fish at days 28–35 post-immunization. The liver of vaccinated flounder exhibited only slight histopathological changes compared with a significant pathology observed in control immunized fish. Additionally, a lower bacterial burden in the liver, spleen, and kidney were observed in pVAA protected fish in response to bacterial challenge, compared with pcDNA3.1 vector control injected fish. Moreover, the pVAA vaccine confers a relative percentage survival of 50.00% following V. anguillarum infection. In summary, this is the first study indicating an initial induction of the T lymphocyte response, followed by B lymphocyte induction of specific antibodies as a result of DNA immunization of flounder. This signifies the important potential of pVAA as a DNA vaccine candidate for the control of V. anguillarum infection.</p

Additional file 3 of NPM3 as a novel oncogenic factor and poor prognostic marker contributes to cell proliferation and migration in lung adenocarcinoma

Additional file 3: Supplementary Figure S3. Single-cell clustering plots and NPM3 expression in GSE131907, GSE143423 andGSE146100 datasets

Additional file 2 of NPM3 as a novel oncogenic factor and poor prognostic marker contributes to cell proliferation and migration in lung adenocarcinoma

Additional file 2: Supplementary Figure S2. NPM3mutation in cancer

Primers used in this work.

<p>Primers used in this work.</p

Quantitative real-time RT-PCR analysis of tissue distribution of <i>FcSUMO</i> and <i>FcUBC9</i> transcripts in healthy <i>F</i>. <i>chinensis</i>.

<p>18S rRNA served as an internal control. Transcription level of the genes in muscle was normalized to 1. The bar graphs represent the relative fold changes of each tissue, together with error bars which represent mean ± standard deviation (n = 3). <i>Different letters</i> indicates significant difference between groups (<i>p</i><0.05). (A) SUMO, (B) UBC9.</p

Primers used in real-time RT-PCR analysis.

<p>Primers used in real-time RT-PCR analysis.</p