5 research outputs found

    the factor-loadings of EFA and CFA

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    <p>a: boldface means significant at 5% level in EFA</p><p>b: all the factor loadings presented were significant at 5% level in CFA</p><p>c: CrI = Credibility Interval estimated by Bayesian estimator</p><p>the factor-loadings of EFA and CFA</p

    The results of the validity test

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    <p>*: p < 0.05,</p><p>**p < 0.01</p><p>a: because of the missing data, the samples in this table are different from the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115948#pone.0115948.t001" target="_blank">Table 1</a></p><p>The results of the validity test</p

    Sensitive and Specific Exonuclease III-Assisted Recombinase-Aided Amplification Colorimetric Assay for Rapid Detection of Nucleic Acids

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    The development of a contamination-free and on-site nucleic acid detection platform with high sensitivity and specificity but low-cost for the detection of pathogenic nucleic acids is critical for infectious disease diagnosis and surveillance. In this study, we combined the recombinase-aided amplification (RAA) with the exonuclease III (Exo III)-assisted signal amplification into a platform for sensitive and specific detection of nucleic acids of African swine fever virus (ASFV). We found that this platform enabled a naked eye visual detection of ASFV at a detection limit as low as 2 copies/μL in 30 min. As expected, no cross-reactivity was observed with other porcine viruses. In addition, to avoid aerosol contamination, a one-tube RAA-Exo III colorimetric assay was also established for the accurate detection of ASFV in clinical samples. Taken together, we developed a rapid, instrument-free, and low-cost Exo III-assisted RAA colorimetric-assay-based nucleic acid detection platform
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