MOESM2 of Comprehensive gene expression analysis for exploring the association between glucose metabolism and differentiation of thyroid cancer

Additional file 2 Figure S2. Box and whisker plot of glycolysis signatures by N-stage or M-stage in PTC. The line across each box represent the median, and the top edge, and the bottom edge represents the first quartile, and the third quartile, respectively. Student’s t-test showed significant difference of signatures of glycolysis between N positive and N negative groups (N negative group 0.13 ± 1.03 vs. N positive group − 0.24 ± 0.89, t = 3.86, p = 0.0001). No significant difference of signatures of glycolysis were found between M positive and M negative groups (M negative group − 0.08 ± 1.03 vs. M positive group − 0.16 ± 0.89, t = 0.22, p = 0.82). (*** = p < 0.001

MOESM1 of Comprehensive gene expression analysis for exploring the association between glucose metabolism and differentiation of thyroid cancer

Additional file 1 Figure S1. Plots for GLUT and glycolysis signatures in PTC with different cell types. (A) Box and whiskers plot of GLUT signature in PTC according to cell type (Classical cell type 20.13 ± 1.32 vs. Follicular cell type 18.42 ± 1.20, t = 11.55, p < 0.001) (B) Box and whiskers plot of glycolysis signature in PTC according to cell type (Classical cell type − 0.13 ± 0.94 vs. Follicular cell type 0.52 ± 1.12, t = − 5.65, p < 0.001) (C) Scatter plot of TDS versus glucose metabolism signature in classical cell type PTC (r = − 0.47, p < 0.001 for GLUT; r = 0.23, p < 0.001 for glycolysis) (D) Scatter plot of TDS versus glucose metabolism signature in follicular cell type PTC (r = 0.43, p < 0.001 for GLUT; r = 0.34, p = 0.001 for glycolysis

Patient characteristics.

Patient characteristics.</p

Correlations of AR with expression of FA β-oxidation-associated genes and GLUT1.

AR expression show positive correlation with FA oxidation (A) and negative correlation with GLUT1 expression (B). AR, expression level of androgen receptor gene; FA, fatty acid; FA oxidation, gene set enrichment score of FA β-oxidation-associated genes; GLUT1, expression level of GLUT1 (SLC2A1) gene.</p

Univariate and multivariate analyses for determining SUV_max.

Univariate and multivariate analyses for determining SUVmax.</p

Univariate and multivariate analyses for determining SUV_max using ≥ 10% for AR positivity.

AR: androgen receptor. (DOCX)</p

Glycolysis-related genes from the REACTOME_GLYCOLYSIS.

(DOCX)</p

Androgen receptor expression according to clinicopathologic characteristics.

Androgen receptor expression according to clinicopathologic characteristics.</p

SUV_max according to androgen receptor status with varying cutoff values for androgen receptor positivity.

AR: androgen receptor. (DOCX)</p

Correlations among AR, SUV_max, and glycolysis-associated genes expressions analyzed using GSE135565 database.

SUVmax was well correlated with glycolysis score (A). A negative correlation between AR expression and SUVmax was observed (B). The AR expression is inversely correlated with glycolysis score (C). AR, expression level of androgen receptor gene; Glycolysis score indicates how close gene expression pattern of a sample is, to expected expression pattern of the glycolysis-related gene set.</p