Adenosine Phosphate Functionalized Magnetic Mesoporous Graphene Oxide Nanocomposite for Highly Selective Enrichment of Phosphopeptides

Developing an efficient strategy to enrich the low abundance phosphopeptides before mass spectrometry detection is a vital preprocessing step in phosphoproteomics. In this work, we synthesized an adenosine phosphate-Ti⁴⁺ functionalized magnetic mesoporous graphene oxide nanocomposite (denoted as MG@mSiO₂-ATP-Ti⁴⁺) to selectively extract phosphorylated peptides from complex biological samples based on the immobilized metal ion affinity chromatography (IMAC). Mesoporous silica was coated on the substrate material of magnetic graphene oxide and then the ATP containing three phosphate groups was grafted on the inwall of mesoporous channels as chelating ligands to immobilize the Ti⁴⁺ cations. With favorable properties, such as large surface area and good hydrophilicity and size-exclusion effect, the MG@mSiO₂-ATP-Ti⁴⁺ exhibited excellent sensitivity and selectivity toward phosphopeptides whether in low concentration of β-casein digest (20 amol μL^–1, 4 fmol) or the digest mixture of β-casein and bovine serum albumin (with molar ratio of 1:1000) as well as good reusability. Furthermore, MG@mSiO₂-ATP-Ti⁴⁺ could also be applied in the selective enrichment of phosphorylated peptides from nonfat milk digest and human saliva and serum

In Vivo Delivery of RNAi by Reducible Interfering Nanoparticles (iNOPs)

RNA interference (RNAi) has considerable potential as a therapeutic strategy, but the development of efficient in vivo RNA delivery methods remains challenging. To this end, we designed and synthesized chemically modified interfering nanoparticles (iNOPs) composed of functionalized poly-l-lysine dendrimers modified with reducible spacers to facilitate release of small interfering RNAs (siRNAs) in vivo. We show that the novel siRNA–iNOP complexes mediate efficient gene-specific RNAi in cultured cells and in mice, where they display enhanced tissue-targeting capabilities. At a clinically feasible dose of 1 mg kg^–1, apolipoprotein B (apoB) siRNA–iNOP complexes achieved ∼40–45% reduction of liver apoB mRNA and plasma apoB protein levels within 48 h of administration to mice, without apparent toxicity. Collectively, these findings demonstrate that siRNA delivery by the modified reducible iNOPs can provide a clinically significant and potentially tissue-specific new approach for RNAi therapy

Preoperative localization for pulmonary nodules: a meta-analysis of coil and liquid materials

This study was designed to conduct pooled comparisons of the relative clinical efficacy and safety of computed tomography (CT)-guided localization for pulmonary nodules (PNs) using either coil- or liquid material-based approaches. Relevant articles published as of July 2023 were identified in the Web of Science, PubMed, and Wanfang databases, and pooled analyses of relevant endpoints were then conducted. Six articles that enrolled 287 patients (341 PNs) and 247 patients (301 PNs) that had respectively undergone CT-guided localization procedures using coil- and liquid material-based approaches prior to video-assisted thoracic surgery (VATS) were included in this meta-analysis. The liquid material group exhibited a significantly higher pooled successful localization rate as compared to the coil group (p = 0.01), together with significantly lower pooled total complication rates (p = 0.0008) and pneumothorax rates (p = 0.01). Both groups exhibited similar rates of pulmonary hemorrhage (p = 0.44) and successful wedge resection (p = 0.26). Liquid-based localization was also associated with significant reductions in pooled localization and VATS procedure durations (p = 0.004 and 0.007). These data are consistent with CT-guided localization procedures performed using liquid materials being safer and more efficacious than coil-based localization in patients with PNs prior to VATS resection.</p

Selection of Amino Acids and the Biomimetic Synthesis of Amido Bond in the Presence of β-CD

<div><p></p><p>A new method was developed to construct a special amido bond in the presence of β-cyclodextrin. This process is similar to peptide synthesis in organisms. NMR experiments were performed to investigate the possible mechanism. This work has potential application in biomimetic peptide synthesis.</p> <p>[Supplementary materials are available for this article. Go to the publisher's online edition of <i>Synthetic Communications</i>® for the following free supplemental resource(s): Full experimental and spectral details.]</p> </div

Effect of polysaccharides extracted from <i>Sipunculus nudus</i> (SNP) on the lifespan and immune damage repair of <i>Drosophila melanogaster</i> exposed to Cd (VI)

<p>The water-soluble polysaccharides extracted from <i>Sipunculus nudus</i> (SNP) was investigated on the lifespan and immune damage repair of <i>Drosophila melanogaster</i> exposed to Cd (VI). SNP increased superoxyde dismutase (SOD), nitrogen monoxide (NO), glutathione peroxidase (GSH-Px) and total anti-oxidation competence (T-AOC), with decreased malondialdehyde (MDA) on <i>D. melanogaster</i> demonstrated that SNP could <i>attenuate</i> <i>oxidative damage of D. melanogaster Exposed to Cd (VI).</i> Real-time PCR and western blot analysis showed that SNP enhanced the gene expression of Diptericin, Drosomycin, Defensin, PGRP-LC and the protein level of Toll, p-JNK and Relish， that suggested the promoting effect of SNP on the immune damage repair of <i>D. melanogaster</i> exposed to Cd (VI). The increased level of Indy, Parkin and AMPK indicated the regulated effect of SNP on the longevity-related pathways through ageing-related moleculars of <i>D. melanogaster</i> exposed to Cd (VI). These results suggested that SNP could also improve the lifespan of <i>D. melanogaster</i> exposed to Cd (VI).</p

Carbonate-Based Zeolitic Imidazolate Framework for Highly Selective CO₂ Capture

In this study, we report the formation of a new crystal structure, ZIF-CO₃-1, which results from the reaction of Zn²⁺, 2-methylimidazole, and carbonate. ZIF-CO₃-1 can be synthesized solvothermally in <i>N</i>,<i>N</i>-dimethylformamide (DMF)/water (H₂O) or by utilizing of CO₂ gas at various temperatures in DMF/H₂O or H₂O. This reaction selectively consumes CO₂ because CO₂ is incorporated in the ZIF as carbonate. CO₂ can be quantitatively released by acidifying the ZIF. Powder X-ray diffraction, single-crystal X-ray diffraction, FTIR spectroscopy, scanning electron microscopy, elemental analysis, and thermogravimetric analysis were used to characterize the ZIF structure. ZIF-CO₃-1 (chemical formula C₉H₁₀N₄O₃Zn₂), crystallizes in the orthorhombic crystal system with noncentrosymmetric space group <i>Pba</i>2

Carbonate-Based Zeolitic Imidazolate Framework for Highly Selective CO₂ Capture

In this study, we report the formation of a new crystal structure, ZIF-CO₃-1, which results from the reaction of Zn²⁺, 2-methylimidazole, and carbonate. ZIF-CO₃-1 can be synthesized solvothermally in <i>N</i>,<i>N</i>-dimethylformamide (DMF)/water (H₂O) or by utilizing of CO₂ gas at various temperatures in DMF/H₂O or H₂O. This reaction selectively consumes CO₂ because CO₂ is incorporated in the ZIF as carbonate. CO₂ can be quantitatively released by acidifying the ZIF. Powder X-ray diffraction, single-crystal X-ray diffraction, FTIR spectroscopy, scanning electron microscopy, elemental analysis, and thermogravimetric analysis were used to characterize the ZIF structure. ZIF-CO₃-1 (chemical formula C₉H₁₀N₄O₃Zn₂), crystallizes in the orthorhombic crystal system with noncentrosymmetric space group <i>Pba</i>2

A Family of Flexible Lanthanide Bipyridinedicarboxylate Metal–Organic Frameworks Showing Reversible Single-Crystal to Single-Crystal Transformations

A family of flexible lanthanide metal–organic frameworks, [Ln₂(bpydc)₃(H₂O)₃]·nDMF (denoted as SUMOF-6-Ln; Ln = Nd, Sm, Eu, Gd, Tb, Dy, Ho, and Er, H₂bpydc =2,2′-bipyridine-5,5′-dicarboxylic acid), was synthesized and characterized. SUMOF-6-Ln has a monoclinic space group <i>P</i>2₁<i>/c</i>. The three-dimensional framework contains chains of LnO_<i>n</i> (<i>n</i> = 7–8) polyhedra connected through the bpydc linkers forming 1D rhombic channels along the <i>c</i>-axis. SUMOF-6-Ln showed reversible breathing phenomenon upon desorption/adsorption of the solvent, with up to 27% changes of the unit cell dimensions and 23% changes of the unit cell volume. Single crystal X-ray diffraction (XRD) revealed that the desolvation and resolvation of SUMOF-6-Ln occurred via single-crystal to single-crystal transformations. The thermal behavior of SUMOF-6-Sm was also examined. SUMOF-6-Eu and SUMOF-6-Tb showed solid-state luminescent properties

A Family of Flexible Lanthanide Bipyridinedicarboxylate Metal–Organic Frameworks Showing Reversible Single-Crystal to Single-Crystal Transformations

A family of flexible lanthanide metal–organic frameworks, [Ln₂(bpydc)₃(H₂O)₃]·nDMF (denoted as SUMOF-6-Ln; Ln = Nd, Sm, Eu, Gd, Tb, Dy, Ho, and Er, H₂bpydc =2,2′-bipyridine-5,5′-dicarboxylic acid), was synthesized and characterized. SUMOF-6-Ln has a monoclinic space group <i>P</i>2₁<i>/c</i>. The three-dimensional framework contains chains of LnO_<i>n</i> (<i>n</i> = 7–8) polyhedra connected through the bpydc linkers forming 1D rhombic channels along the <i>c</i>-axis. SUMOF-6-Ln showed reversible breathing phenomenon upon desorption/adsorption of the solvent, with up to 27% changes of the unit cell dimensions and 23% changes of the unit cell volume. Single crystal X-ray diffraction (XRD) revealed that the desolvation and resolvation of SUMOF-6-Ln occurred via single-crystal to single-crystal transformations. The thermal behavior of SUMOF-6-Sm was also examined. SUMOF-6-Eu and SUMOF-6-Tb showed solid-state luminescent properties

DataSheet1_Qualitative and quantitative chemical analysis of Leptadenia hastata: exploring a traditional african medicinal plant.docx

Leptadenia hastata (Pers.) Decne is a commonly used food source and prescribed as a traditional African medicine for treatment of various diseases, such as diabetes, skin disorders, wounds, and ulcers. However, quality control has become a bottleneck restricting the therapeutic development and utilization of this plant. In this study, a reliable method for qualitative and quantitative determination of components in Leptadenia hastata was established. The components of L. hastata were profiled using ultra-high performance liquid chromatography coupled with quadruple time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS). Subsequently, an ultra-high performance tandem diode array detector (UHPLC-DAD)-based method was used for simultaneous quantitative analysis of five major constituents in six batches of L. hastata samples. As a result, 35 compounds were tentatively identified. The quantities of the five constituents (vicenin-Ⅱ, orientin, schaftoside, chrysin 6-C-arabinoside 8-C-glucoside, chrysin 6-C-glucoside 8-C-arabinoside) were determined as 124.8–156.9 μg/g, 170.5–216.0 μg/g, 61.31–93.73 μg/g, 85.13–119.3 μg/g and 99.82–129.4 μg/g, respectively. This method offers a successful strategy for precise and effective evaluation of the constituents of L. hastata, providing a robust foundation for holistic quality assessment of medicinal plants.</p