Image1_Analysis of risk factors for postoperative recurrence of stage I colorectal cancer: a retrospective analysis of a large population.jpg

BackgroundColorectal cancer (CRC) is the third most common cancer worldwide. Patients diagnosed with stage I CRC typically do not require postoperative adjuvant treatment. However, postoperative recurrence is present in at least 40% of patients with CRC and often occurs in those with stage I disease. This study aimed to elucidate the current status of recurrence and clinicopathological characteristics in patients with stage I CRC.MethodsData of indicated patients were obtained from 18 registries in Surveillance, Epidemiology, and End Results (SEER). The multivariable Fine–Gray regression model was used to identify the mortality risk of patients. Disparities in survival were analyzed using Kaplan–Meier curves. Logistic regression was employed to identify factors associated with recurrent risk overestimation.ResultsOur study indicated a recurrence rate of 15.04% (1,874/12,452) in stage I CRC cases. Notably, we identified race, age, T stage, and carcinoembryonic antigen (CEA) levels as independent risk factors for tumor recurrence, substantially impacting prognosis. Furthermore, gender, race (Black), age (>65 years), elevated CEA levels, and refusal or unknown status regarding radiotherapy significantly correlated with an adverse prognosis in patients with stage I CRC.ConclusionsWe identified certain key clinicopathological features of patients with stage I CRC and demonstrated the survival benefits of radiotherapy, offering a new perspective on stage I CRC follow-up and treatment recommendations.</p

Amplification of CD20 Cross-Linking in Rituximab-Resistant B‑Lymphoma Cells Enhances Apoptosis Induction by Drug-Free Macromolecular Therapeutics

Although the CD20-targeted monoclonal antibody rituximab (RTX) has revolutionized the therapeutic landscape for B-cell malignancy, relapsed and refractory disease due to RTX resistance continue to constitute major challenges, illustrating the need for better therapies. Here, we apply drug-free macromolecular therapeutics (DFMT) that amplifies CD20 cross-linking to enhance apoptosis in RTX-resistant cells. Bispecific engager (anti-CD20 Fab′ conjugated with oligonucleotide1) pretargets CD20 and the deletion of Fc-region minimizes its premature endocytosis in resistant cells that rapidly internalize and consume CD20/RTX complexes. Second-step delivery of multivalent polymeric effector (linear copolymer conjugated with multiple copies of complementary oligonucleotide 2) simultaneously hybridizes multiple CD20-bound engagers and strengthens CD20 ligation. Moreover, the restoration of CD20 expression by the pretreatment of cells with a polymer-gemcitabine conjugate, a CD20 expression enhancer, unleashes the full potential of DFMT in the CD20-deficient resistant cells. Hence, amplification of CD20 cross-linking is achieved by (1) the enhancement of surface CD20 accessibility, (2) the increase in CD20 expression, and (3) multimeric CD20 binding, which ultimately translates into the amplified activation of a wide range of innate apoptotic responses. We demonstrated that the altered molecular signaling pathway that originally results in RTX resistance could be circumvented and compensated by other DFMT-augmented pathways. Of note, our preliminary data provide proof-of-concept that CD20 cross-linking amplification emerges as an important strategy for overcoming RTX resistance

Supplementary document for Integrated programmable fully-coupled three bus-ring resonator photonic molecule with ultra-low power piezo-electric control - 6342980.pdf

Supplemental Inf

Placenta Growth Factor in Eyes with Neovascular Glaucoma Is Decreased after Intravitreal Ranibizumab Injection

<div><p>Purpose</p><p>To evaluate changes in the concentrations of placental growth factor (PlGF) and vascular endothelial growth factor-A (VEGF-A) in aqueous humor of patients with neovascular glaucoma (NVG) before and after an intravitreal injection of ranibizumab (IVR) and to determine the underlying correlation between the levels.</p><p>Methods</p><p>The prospective interventional comparative study involved 20 eyes of 20 patients with surgery-required advanced NVG and 20 control subjects from January 2013 to November 2013. The NVG eyes received the IVR treatment before glaucoma surgery. Aqueous humor was collected at the time of the IVR injection (pre- IVR) and at the time of antiglaucomatous surgery (post-IVR). Aqueous humor was also collected at the time of cataract surgery in normal control. Aqueous humor and plasma VEGF-A and PlGF levels were measured with an enzyme-linked immunosorbent assay methods, respectively.</p><p>Results</p><p>The mean aqueous humor PlGF and VEGF-A concentrations in the pre-IVR eyes were significantly higher than in those of the control subjects (p<0.001), whereas the plasma levels showed no significant difference. There was a statistically significant correlation between the aqueous humor PlGF and the VEGF-A concentration (<i>r</i> = 0.612, p = 0.003). The mean aqueous humor PlGF in the post-IVR eyes dramatically decreased from 1078.36 ± 755.83 to 177.64 ± 151.73 pg/mL (p<0.001). The VEGF-A level showed a similar trend from 3697.64 ± 2104.47 pg/mL to 183.54 ± 130.35 pg/mL (p<0.001).</p><p>Conclusions</p><p>Aqueous humor concentrations of VEGF-A and PlGF were significantly elevated in the eyes with NVG, and there was a positive correlation between the levels. After an IVR treatment, VEGF-A and PlGF were significantly decreased in NVG eyes.</p></div

Aqueous humor VEGF-A concentration in the pre-IVR and post-IVR NVG eyes.

<p>Compared to pre-IVR, the mean aqueous humor VEGF-A concentration significantly decreased post-IVR in the NVG eyes (*p<0.001). Each point represents a measurement from a single patient.</p

PlGF levels of the aqueous humor.

<p>The PlGF levels of the aqueous humor were significantly different between the groups (*p<0.001), whereas there was no significant difference in serum PlGF concentrations between the groups (p = 0.115).</p

VEGF-A levels of the aqueous humor of the groups.

<p>The VEGF-A levels of the aqueous humor were significantly different between the groups (*p<0.001), whereas there was no significant difference in serum VEGF-A concentrations between the groups (p = 0.636).</p

Novel Top-Contact Monolayer Pentacene-Based Thin-Film Transistor for Ammonia Gas Detection

We report on the fabrication of an organic field-effect transistor (OFET) of a monolayer pentacene thin film with top-contact electrodes for the aim of ammonia (NH₃) gas detection by monitoring changes in its drain current. A top-contact configuration, in which source and drain electrodes on a flexible stamp [poly­(dimethylsiloxane)] were directly contacted with the monolayer pentacene film, was applied to maintain pentacene arrangement ordering and enhance the monolayer OFET detection performance. After exposure to NH₃ gas, the carrier mobility at the monolayer OFET channel decreased down to one-third of its original value, leading to a several orders of magnitude decrease in the drain current, which tremendously enhanced the gas detection sensitivity. This sensitivity enhancement to a limit of the 10 ppm level was attributed to an increase of charge trapping in the carrier channel, and the amount of trapped states was experimentally evaluated by the threshold voltage shift induced by the absorbed NH₃ molecular analyte. In contrast, a conventional device with a 50-nm-thick pentacene layer displayed much higher mobility but lower response to NH₃ gas, arising from the impediment of analyte penetrating into the conductive channel, owing to the thick pentacene film

Highly Interpenetrated Robust Microporous Hydrogen-Bonded Organic Framework for Gas Separation

A hydrogen-bonded organic framework (HOF), HOF-11, has been successfully prepared by the slow diffusion of hexane into a tetrahydrofuran solution of tris­(4-carboxyphenyl)­amine (TCPA). HOF-11 has been characterized by single-crystal and powder X-ray diffraction analysis, which is composed of TCPA ligands connected by the intermolecular hydrogen-bonding interactions in the carboxylic group dimer, showing 11-fold interpenetrated three-dimensional hydrogen-bonded networks in a (10,3)-b topology with the pore size of 6.2 × 6.8 Ų. The permanent porosity of degassed HOF was demonstrated by virtue of the CO₂ sorption and selective gas adsorption

Brain MRI changes and CDMS conversion.

<p>UON, unilateral optic neuritis; n, number; DIS, dissemination of the lesion in space; DIT, dissemination of the lesion over time.</p><p>Brain MRI changes and CDMS conversion.</p