Convenient and Practical Synthesis of (<i>R</i>)-(+)-4-Methyl-2-cyclohexen-1-one

Convenient and Practical Synthesis of (R)-(+)-4-Methyl-2-cyclohexen-1-on

DataSheet_1_Genome-wide characterization of the soybean DOMAIN OF UNKNOWN FUNCTION 679 membrane protein gene family highlights their potential involvement in growth and stress response.docx

The DMP (DUF679 membrane proteins) family is a plant-specific gene family that encodes membrane proteins. The DMP family genes are suggested to be involved in various programmed cell death processes and gamete fusion during double fertilization in Arabidopsis. However, their functional relevance in other crops remains unknown. This study identified 14 genes from the DMP family in soybean (Glycine max) and characterized their physiochemical properties, subcellular location, gene structure, and promoter regions using bioinformatics tools. Additionally, their tissue-specific and stress-responsive expressions were analyzed using publicly available transcriptome data. Phylogenetic analysis of 198 DMPs from monocots and dicots revealed six clades, with clade-I encoding senescence-related AtDMP1/2 orthologues and clade-II including pollen-specific AtDMP8/9 orthologues. The largest clade, clade-III, predominantly included monocot DMPs, while monocot- and dicot-specific DMPs were assembled in clade-IV and clade-VI, respectively. Evolutionary analysis suggests that soybean GmDMPs underwent purifying selection during evolution. Using 68 transcriptome datasets, expression profiling revealed expression in diverse tissues and distinct responses to abiotic and biotic stresses. The genes Glyma.09G237500 and Glyma.18G098300 showed pistil-abundant expression by qPCR, suggesting they could be potential targets for female organ-mediated haploid induction. Furthermore, cis-acting regulatory elements primarily related to stress-, hormone-, and light-induced pathways regulate GmDMPs, which is consistent with their divergent expression and suggests involvement in growth and stress responses. Overall, our study provides a comprehensive report on the soybean GmDMP family and a framework for further biological functional analysis of DMP genes in soybean or other crops.</p

DataSheet_2_Genome-wide characterization of the soybean DOMAIN OF UNKNOWN FUNCTION 679 membrane protein gene family highlights their potential involvement in growth and stress response.zip

The DMP (DUF679 membrane proteins) family is a plant-specific gene family that encodes membrane proteins. The DMP family genes are suggested to be involved in various programmed cell death processes and gamete fusion during double fertilization in Arabidopsis. However, their functional relevance in other crops remains unknown. This study identified 14 genes from the DMP family in soybean (Glycine max) and characterized their physiochemical properties, subcellular location, gene structure, and promoter regions using bioinformatics tools. Additionally, their tissue-specific and stress-responsive expressions were analyzed using publicly available transcriptome data. Phylogenetic analysis of 198 DMPs from monocots and dicots revealed six clades, with clade-I encoding senescence-related AtDMP1/2 orthologues and clade-II including pollen-specific AtDMP8/9 orthologues. The largest clade, clade-III, predominantly included monocot DMPs, while monocot- and dicot-specific DMPs were assembled in clade-IV and clade-VI, respectively. Evolutionary analysis suggests that soybean GmDMPs underwent purifying selection during evolution. Using 68 transcriptome datasets, expression profiling revealed expression in diverse tissues and distinct responses to abiotic and biotic stresses. The genes Glyma.09G237500 and Glyma.18G098300 showed pistil-abundant expression by qPCR, suggesting they could be potential targets for female organ-mediated haploid induction. Furthermore, cis-acting regulatory elements primarily related to stress-, hormone-, and light-induced pathways regulate GmDMPs, which is consistent with their divergent expression and suggests involvement in growth and stress responses. Overall, our study provides a comprehensive report on the soybean GmDMP family and a framework for further biological functional analysis of DMP genes in soybean or other crops.</p

Li₂NiO₂ as a Novel Cathode Additive for Overdischarge Protection of Li-Ion Batteries

Li2NiO2 as a Novel Cathode Additive for Overdischarge Protection of Li-Ion Batterie

Effect of androgen-deprivation therapy (ADT) on the longitudinal serum uric acid (SUA) change in the whole population and post-matched population.

Effect of androgen-deprivation therapy (ADT) on the longitudinal serum uric acid (SUA) change in the whole population and post-matched population.</p

The generation of new mosaic CTX phages from V212-1 by inter-strand recombination between CTX phages and intra-strand recombination between CTX-1 and RS1 on chromosome 1.

<p>A double crossover recombination event between 2 prophages on each chromosome of V212-1 (indicated by arrows) results in the generation of the CTX-1* prophage which contains <i>ctxB</i>^cla on chromosome 1. Intra-stand recombination between CTX-1* and RS1 generates a mosaic CTX prophage. Depending on the recombination position (shown as R1, R2, and R3), CTX-3, -5, and -6 can be generated (CTX-4 can also be generated, but not shown in this figure). The infectious CTX-3, -5, or -6 virions can be transduced to a new host to give rise to Wave 3 strains.</p

Longitudinal changes in serum urate (SUA) levels according to the subgroups.

Hyperuricemia (SUA ≥7.0 mg/dL), normouricemia (4.0≤ and <7.0 mg/dL), and hypouricemia (<4.0 mg/dL) were defined by using the baseline SUA levels. P values were corrected by the Bonferroni method. * p <0.05 between the baseline and 6-month time points; †, p <0.05 when compared between the surgery and ADT groups at each time point.</p

Effect of the interaction between a specific clinical factor and time on the longitudinal serum uric acid (SUA) levels.

Effect of the interaction between a specific clinical factor and time on the longitudinal serum uric acid (SUA) levels.</p

Flowchart for patient inclusion.

*, including stomach cancer (n = 2), hepatocellular cancer (1), colon cancer (1), multiple myeloma (1), gastrointestinal stromal tumor (1), and ulcerative colitis (1). ADT, androgen deprivation therapy.</p

Molecular Insights Into the Evolutionary Pathway of <i>Vibrio cholerae</i> O1 Atypical El Tor Variants

<div><p>Pandemic <i>V. cholerae</i> strains in the O1 serogroup have 2 biotypes: classical and El Tor. The classical biotype strains of the sixth pandemic, which encode the classical type cholera toxin (CT), have been replaced by El Tor biotype strains of the seventh pandemic. The prototype El Tor strains that produce biotype-specific cholera toxin are being replaced by atypical El Tor variants that harbor classical cholera toxin. Atypical El Tor strains are categorized into 2 groups, Wave 2 and Wave 3 strains, based on genomic variations and the CTX phage that they harbor. Whole-genome analysis of <i>V. cholerae</i> strains in the seventh cholera pandemic has demonstrated gradual changes in the genome of prototype and atypical El Tor strains, indicating that atypical strains arose from the prototype strains by replacing the CTX phages. We examined the molecular mechanisms that effected the emergence of El Tor strains with classical cholera toxin-carrying phage. We isolated an intermediary <i>V. cholerae</i> strain that carried two different CTX phages that encode El Tor and classical cholera toxin, respectively. We show here that the intermediary strain can be converted into various Wave 2 strains and can act as the source of the novel mosaic CTX phages. These results imply that the Wave 2 and Wave 3 strains may have been generated from such intermediary strains in nature. Prototype El Tor strains can become Wave 3 strains by excision of CTX-1 and re-equipping with the new CTX phages. Our data suggest that inter-chromosomal recombination between 2 types of CTX phages is possible when a host bacterial cell is infected by multiple CTX phages. Our study also provides molecular insights into population changes in <i>V. cholerae</i> in the absence of significant changes to the genome but by replacement of the CTX prophage that they harbor.</p></div