Prindle Post Education Edition: Journalism Winter 2022

The Prindle Post is the Prindle Institute for Ethics’s online source for pieces that examine and explain the ethical dimensions of current events and culture. The Prindle Institute regularly produces print editions containing some of our favorite Prindle Post articles on a given topic. Our latest edition covers topics related to journalism. Each Prindle Post workbook includes comprehension and focus questions for each article, as well as vocabulary and activity support. Contents: Mission Statement - Andrew Cullison How To Use This Book - Eleanor Price How To Talk About Ethics in the Classroom - Tucker Sechrest How To Create and Use a Stakeholder Map - Kara Jedele A Note on Objectives and Standards for Teachers - Kara Jedele Main Body 1. In the Limelight: Ethics for Journalists as Public Figures - Abigail McArthur-Self 2. Objectivity in Journalism - Benjamin Rossi 3. On Journalistic Malpractice - A.G. Holdier 4. Should News Sites Have Paywalls? - Beatrice Harvey 5. Abusing Public Trust: Brooks, Gladwell, and Journalistic Ethics - Tucker Sechrest 6. Is the Future of News a Moral Question? - Matthew S.W. Sil

Prindle Post Print Edition 2020: Democracy

The Prindle Post is the Prindle Institute for Ethics’s online source for pieces that examine and explain the ethical dimensions of current events and culture. The Prindle Institute regularly produces print editions containing some of our favorite Prindle Post articles on a given topic. Our latest edition covers topics related to democracy. Each Prindle Post workbook includes comprehension and focus questions for each article, as well as vocabulary and activity support. Contents: Mission Statement - Andrew Cullison How To Use This Book - Eleanor Price How To Talk About Ethics in the Classroom - Tucker Sechrest How To Create and Use a Stakeholder Map - Kara Jedele A Note on Objectives and Standards for Teachers - Kara Jedele Main Body 1. “Is the Filibuster Democratic?” - Alex Layton 2. On the Question of Strategic Voting - Matthew Silk 3. Women, Representation, Revolution - Rachel Robison-Greene 4. What’s Wrong with State Media? - Andrew Bobker 5. Press Freedom in Australia: Democracy, Transparency, and Trust - Desmonda Lawrence 6. To Keep or Not to Keep? Electoral College and Presidential Representativeness - Evan Butt

Prindle Post Education Edition: Climate Change Spring 2021

The Prindle Post is the Prindle Institute for Ethics’s online source for pieces that examine and explain the ethical dimensions of current events and culture. The Prindle Institute regularly produces print editions containing some of our favorite Prindle Post articles on a given topic. Our latest edition covers topics related to climate change. Each Prindle Post workbook includes comprehension and focus questions for each article, as well as vocabulary and activity support. Contents: Mission Statement - Andrew Cullison How To Use This Book - Eleanor Price How To Talk About Ethics in the Classroom - Tucker Sechrest How To Create and Use a Stakeholder Map - Kara Jedele A Note on Objectives and Standards for Teachers - Kara Jedele Main Body 1. Ethical Concepts in the Age of Anthropocene - Desmonda Lawrence 2. Fixing What We’ve Broken: Geoengineering in Response to Climate Change Rachel Robison-Greene 3. Collective Action and Climate Change: Consumption, Defection, and Motivation - Meredith McFadden 4. Discussing Scientific Consensus on Climate Change - Matthew Silk 5. Procreative Autonomy and Climate Change - Smriti Kark

Identification and Evaluation of Novel Acetolactate Synthase Inhibitors as Antifungal Agents

High-throughput phenotypic screening against the yeast Saccharomyces cerevisiae revealed a series of triazolopyrimidine-sulfonamide compounds with broad-spectrum antifungal activity, no significant cytotoxicity, and low protein binding. To elucidate the target of this series, we have applied a chemogenomic profiling approach using the S. cerevisiae deletion collection. All compounds of the series yielded highly similar profiles that suggested acetolactate synthase (Ilv2p, which catalyzes the first common step in branched-chain amino acid biosynthesis) as a possible target. The high correlation with profiles of known Ilv2p inhibitors like chlorimuron-ethyl provided further evidence for a similar mechanism of action. Genome-wide mutagenesis in S. cerevisiae identified 13 resistant clones with 3 different mutations in the catalytic subunit of acetolactate synthase that also conferred cross-resistance to established Ilv2p inhibitors. Mapping of the mutations into the published Ilv2p crystal structure outlined the chlorimuron-ethyl binding cavity, and it was possible to dock the triazolopyrimidine-sulfonamide compound into this pocket in silico. However, fungal growth inhibition could be bypassed through supplementation with exogenous branched-chain amino acids or by the addition of serum to the medium in all of the fungal organisms tested except for Aspergillus fumigatus. Thus, these data support the identification of the triazolopyrimidine-sulfonamide compounds as inhibitors of acetolactate synthase but suggest that targeting may be compromised due to the possibility of nutrient bypass in vivo