Diseases of treated cows and the TW per vaccinated cow in which diseases emerged.

<p>Diseases of treated cows and the TW per vaccinated cow in which diseases emerged.</p

Milk yield and proximates related to different groups and lactation stages.

<p>Milk yield and proximates related to different groups and lactation stages.</p

Anti-<i>C</i>. <i>diff</i>. milk IgA contents in the different lactation stages.

<p>BTC means the "before treatment control" value of all cows in frame for vaccination. The lactation stages are defined as “early” for ≤ 100 DiL, “mid” for 101–200 DiL and “late” for ≥ 201 DiL. On the ordinate, <i>C</i>. <i>diff</i>. specific milk IgA concentrations are depicted as LSM ± SD and presented comparatively for the BTC (n = 9, black bar), low responder (LR, n = 4, cross-striped bars) and high responder (HR, n = 5, dotted bars) groups. Substantial or highly significant differences versus BTC and between LR and HR in the same lactation stage are marked by brackets with one (P < 0.05) or two asterisks (P < 0.01). Significant differences within the same group related to the early lactation stage are shown in lower case letters (a, b) for P < 0.05.</p

Anti-<i>C</i>. <i>diff</i>. IgA concentrations in milk and in blood during the 31-week treatment period.

<p>The ordinate values in milk (solid lines, top) and in blood (broken lines, bottom) are shown as LSM ± SD per treated groups, the low responder (LR, n = 4, triangles) and the high responder (HR, n = 5, circles). Substantial or highly significant differences between both groups at the same point in time are marked by one (P < 0.05) or two asterisks (P < 0.01). The gray bars indicate the one-time application of <i>MucoCD-I</i> vaccine batch A in TW 3 and the first use of vaccine batch B for injection in TW 17. The vaccination routes were nasal (N), percutaneous (PC), subcutaneous (SC) and intracutaneous (IC). The injected <i>MucoCD-I</i> vaccine was uniquely supplemented with incomplete Freund’s adjuvant (iFA).</p

Total IgA concentrations in milk and in blood during 31-weeks treatment period.

Measured total IgA values in milk (solid lines, top) and in blood (broken lines, bottom) are shown as LSM ± SD per treated groups, the low responder (LR, n = 4, triangles) and the high responder (HR, n = 5, circles), on the ordinate. Substantial or highly significant differences between both groups compared to the same point in time are marked by one (P MucoCD-I vaccine batch A in TW 3 and the first use of vaccine batch B for injection in TW 17. The vaccination routes were nasal (N), PC, SC and IC. The injected MucoCD-I vaccine was uniquely supplemented with iFA.</p

Immunization schedule.

<p>Immunization schedule.</p

MOESM5 of Immune cell counts and signaling in body fluids of cows vaccinated against Clostridium difficile

Additional file 5: Table S5. Total SC counts and the relative distribution of the different leukocyte populations in milk

MOESM3 of Immune cell counts and signaling in body fluids of cows vaccinated against Clostridium difficile

Additional file 3: Table S3. ΔΔCq changes in gene expression of SC relative to TW 1 or rather TW 15

MOESM2 of Immune cell counts and signaling in body fluids of cows vaccinated against Clostridium difficile

Additional file 2: Table S2. ΔΔCq changes in gene expression of PBL relative to TW 0 or rather TW 16

Total milk IgA contents in the different lactation stages.

<p>BTC means the "before treatment control" value of all cows in frame for vaccination. The lactation stages are defined as “early” for ≤ 100 DiL, “mid” for 101–200 DiL, “late” for ≥ 201 DiL. The ordinate values represent comparatively LSM ± SD of the BTC (n = 9, black bar), low responder (LR, n = 4, cross-striped bars) and high responder (HR, n = 5, dotted bars) groups. Brackets with asterisks link treated groups with BTC and during the same lactation stage, if significant differences between them are indicated by P < 0.05 (*) or P < 0.01 (**). Significant differences within the same group related to the early lactation stage are indicated by lower case letters (a, b) in case of P < 0.05 and upper case letters (A, B) for P < 0.01.</p