The Use Of Hands In Creative Process: Why We Need To Develop Our Hand Skills

 This Major Research Project aims to discuss the significance of the use of hands in creative processes in fashion design practice. In particular, hands are the essential tools to experience and understand materials in creative processes as one of the most primary organs to stimulate haptic experience. However, the significance of the role of hands in creative practice is diminishing because of conditioned use of advanced technology and devises that are controlled by simplified hand motions. Therefore, by conducting a practice-based research method for a series of experimentations, this study aims to explore the significance of the use of hands in the creative process in fashion design practice. The experiments are composed of four different techniques using simple and repetitive hand motions to explore an intimate relationship between the maker, technique and materials employed in the making process. By practicing the techniques, this study aims to provide: I. How the use of hands plays a significant role in the creative process, II. How the use of hands can guide the maker’s design direction and help them form their aesthetic values through the maker’s phenomenological experience, and III. How the use of our hands can help our idea generation and understanding in the design process. Ultimately, this study will connect the findings to creative higher education, specifically in the area of fashion, and provide a vision of the use of hands as one of the critical intelligence training in creative studies.  Keywords: Creative process, the use of hands, haptic experience, dexterity, aesthetic value, creative education, fashion/garment design in fashion studies </p

Direct Detection of α‑1 Antitrypsin in Serum Samples using Surface Plasmon Resonance with a New Aptamer–Antibody Sandwich Assay

The challenges associated with performing surface plasmon resonance (SPR) based measurements in serum and other biofluids have continued to limit the applicability of this valuable sensing technology for sensitive bioaffinity measurements of proteins in clinically relevant samples. In this paper, a new sandwich assay is introduced for the quantitative SPR analysis of α-1 antitrypsin (AAT), which is a recognized biomarker for Alzheimer’s disease. Detection was performed via the specific adsorption of AAT onto a gold chip surface modified with a DNA aptamer. The measurement dynamic range and also sensitivity in serum were improved with the subsequent surface binding of antiAAT. A methodology was established to measure the target protein in serum, albumin and immunoglobulin G (IgG) solutions with the results correlated with measurements in buffer only. A comparison between SPR and enzyme-linked immunosorbent assay (ELISA) measurements was also made. The detection of AAT in serum at clinically relevant concentrations was demonstrated with target concentrations as low as 10 fM readily achievable

<i>Trans</i>-2,5-Disubstituted Tetrahydrofurans via Addition of Carbon Nucleophiles to the Strained Bicyclic Acetal 2,7-Dioxabicyclo[2.2.1]heptane

Addition of allyltributylstannane to 2,7-dioxabicyclo[2.2.1]heptane in the presence of TiCl4 produces 5-allyl-2-(hydroxymethyl)tetrahydrofuran with a trans/cis ratio of 93:7. The trans-selectivity is also observed in additions of various other carbon nucleophiles

Fig 3 -

Relative importance as predictors of IOS variables for PFT parameters; A) FEV1 B) FEF25-75% C) Raw% (D) sRaw%.</p

Cut off, sensitivity, specificity, and their area under the curve with 95% CI of IOS and plethysmographic parameters for the diagnosis of BO.

Cut off, sensitivity, specificity, and their area under the curve with 95% CI of IOS and plethysmographic parameters for the diagnosis of BO.</p

Direct Detection of Genomic DNA by Enzymatically Amplified SPR Imaging Measurements of RNA Microarrays

A novel surface enzymatic reaction scheme that amplifies the optical response of RNA microarrays to the binding of complementary DNA is developed for the direct detection and analysis of genomic DNA. The enzyme RNase H is shown to selectively and repeatedly destroy RNA from DNA−RNA heteroduplexes on gold surfaces; when used in conjunction with the label-free technique of surface plasmon resonance (SPR) imaging, DNA oligonucleotides can be detected at a concentration of 1 fM. This enzymatically amplified SPR imaging methodology is then utilized to detect and identify the presence of the TSPY gene in human genomic DNA without PCR amplification

Fig 2 -

Linear regression plot between PFTs and log-transformed IOS parameters; A-C) Correlation with FEV1; (A) R5-20 cmH2O/L/s (B) R5% (C) AX %, D-F) correlation with Raw %; (D) R5-20 cmH2O/L/s (E) R5% (F) AX %.</p

Detection of Protein Biomarkers Using RNA Aptamer Microarrays and Enzymatically Amplified Surface Plasmon Resonance Imaging

A methodology for the detection of protein biomarkers at picomolar concentrations that utilizes surface plasmon resonance imaging (SPRI) measurements of RNA aptamer microarrays is developed. The adsorption of proteins onto the RNA microarray is detected by the formation of a surface aptamer−protein−antibody complex. The SPRI response signal is then amplified using a localized precipitation reaction catalyzed by the enzyme horseradish peroxidase that is conjugated to the antibody. This enzymatically amplified SPRI methodology is first characterized by the detection of human thrombin at a concentration of 500 fM; the appropriate thrombin aptamer for the sandwich assay is identified from a microarray of three potential thrombin aptamer candidates. The SPRI method is then used to detect the protein vascular endothelial growth factor (VEGF) at a biologically relevant concentration of 1 pM. VEGF is a signaling protein that has been used as a serum biomarker for rheumatoid arthritis, breast cancer, lung cancer, and colorectal cancer and is also associated with age-related macular degeneration

Patient characteristics in all patients.

Patient characteristics in all patients.</p

Dual Nanoparticle Amplified Surface Plasmon Resonance Detection of Thrombin at Subattomolar Concentrations

A novel dual nanoparticle amplification approach is introduced for the enhanced surface plasmon resonance (SPR) detection of a target protein at subattomolar concentrations. Thrombin was used as a model target protein as part of a sandwich assay involving an antithrombin (anti-Th) modified SPR chip surface and a thrombin specific DNA aptamer (Th-aptamer) whose sequence also includes a polyadenine (A30) tail. Dual nanoparticle (NP) enhancement was achieved with the controlled hybridization adsorption of first polythymine-NP conjugates (T20-NPs) followed by polyadenine-NPs (A30-NPs). Two different nanoparticle shapes (nanorod and quasi-spherical) were explored resulting in four different NP pair combinations being directly compared. It was found that both the order and NP shape were important in optimizing the assay performance. The use of real-time SPR measurements to detect target concentrations as low as 0.1 aM is a 10-fold improvement compared to single NP-enhanced SPR detection methods