Data statistics of small RNAs produced by Illumina deep sequencing.

<p>Data statistics of small RNAs produced by Illumina deep sequencing.</p

The occurrence of <i>A</i>. <i>catenella</i> miRNAs that appeared also in other selected species.

<p>Gma: <i>Glycine max</i>, osa: <i>Oryza sativa</i>, ptc: <i>Populus trichocarpa</i>, mdm: <i>Malus domestica</i>, zma: <i>Zea mays</i>, mes: <i>Manihot esculenta</i>, vvi: <i>Vitis vinifera</i>, lus: <i>Linum usitatissimum</i>, aly: <i>Arabidopsis lyrata</i>, nta: <i>Nicotiana tabacum</i>, ath: <i>Arabidopsis thaliana</i>, cme: <i>Cucumis melo</i>, bdi: <i>Brachypodium distachyon</i>, sbi: <i>Sorghum bicolor</i>, mtr: <i>Medicago truncatula</i>, stu: <i>Solanum tuberosum</i>, tcc: <i>Theobroma cacao</i>, rco: <i>Ricinus communis</i>, bna: <i>Brassica napus</i>, ppt: <i>Physcomitrella patens</i>, bra: <i>Brachypodium distachyon</i>, sly: <i>Solanum lycopersicum</i>, cpa: <i>Carica papaya</i>, ghr: <i>Gossypium hirsutum</i>, aqc: <i>Aquilegia caerulea</i>, hvu: <i>Hordeum vulgare</i>, Gma: <i>Glycine max</i>, osa: <i>Oryza sativa</i>, ptc: <i>Populus trichocarpa</i>, mdm: <i>Malus domestica</i>, zma: <i>Zea mays</i>, mes: <i>Manihot esculenta</i>, vvi: <i>Vitis vinifera</i>, lus: <i>Linum usitatissimum</i>, aly: <i>Arabidopsis lyrata</i>, nta: <i>Nicotiana tabacum</i>, ath: <i>Arabidopsis thaliana</i>, cme: <i>Cucumis melo</i>, bdi: <i>Brachypodium distachyon</i>, sbi: <i>Sorghum bicolor</i>, mtr: <i>Medicago truncatula</i>, stu: <i>Solanum tuberosum</i>, tcc: <i>Theobroma cacao</i>, rco: <i>Ricinus communis</i>, bna: <i>Brassica napus</i>, ppt: <i>Physcomitrella patens</i>, bra: <i>Brachypodium distachyon</i>, sly: <i>Solanum lycopersicum</i>, cpa: <i>Carica papaya</i>, ghr: <i>Gossypium hirsutum</i>, aqc: <i>Aquilegia caerulea</i>, hvu: <i>Hordeum vulgare</i>.</p

qPCR validation of the differentially expressedtae-miR159a.

<p>qPCR validation of the differentially expressedtae-miR159a.</p

Novel miRNAs predicted in <i>A</i>. <i>catenella</i>.

<p>Novel miRNAs predicted in <i>A</i>. <i>catenella</i>.</p

miRNA families with more than two members.

<p>miRNA families with more than two members.</p

Venn diagram of identified known miRNAs in the two libraries.

<p>The diagram not only shows the numbers of miRNA that were expressed in the lag phase and the logarithmic phase preferentially but also the co-expressed miRNAs in both phases.</p

Twelve differentially expressed miRNAs in two different growth phases in <i>A</i>. <i>catenella</i>.

<p>log₂ (fold change):log₂ (the normalized expression in the lag phase /the normalized expression in the logarithmic phase); R+1 means the miRNA_seq (detected) is one base more than known rep_miRSeq on the right side; L-1 means the miRNA_seq (detected) is one base less than known rep_miRSeq on the left side; 1ss11GC means one substitution (ss), where G was substituted by C at position 11.</p><p>Twelve differentially expressed miRNAs in two different growth phases in <i>A</i>. <i>catenella</i>.</p

The significantly enriched KEGG pathways of target genes for 12 differentially expressed miRNAs.

<p>The significantly enriched KEGG pathways of target genes for 12 differentially expressed miRNAs.</p

First nucleotide bias of known miRNAs in <i>A</i>. <i>catenella</i>.

<p>The figure indicates the first nucleotide of 18–25 nt miRNAs. U had the greatest frequency among miRNAs of 20–23 nt.</p

Expression abundance of miRNA families in two growth phases in <i>A</i>. <i>catenella</i>.

<p>X-axis represents the known miRNA families, Y-axis represents the normalized expression level of each miRNA family.</p