Figures of Song et al, JBC, 2021 (PMCID: PMC8498002)

This file contains Fig.1 to Fig. 6 from Song et al, JBC, 2021 (PMCID: PMC8498002).</p

Cytology of the ACC hybrid and its microspore-derived lines from <i>B</i>. <i>napus</i> and <i>B</i>. <i>oleracea</i>.

<p>(A) One cell of ACC with chromosome number 28; (B) One PMC of ACC with 9II + 10I; (C) One PMC of ACC with 10II + 8I; (D) One PMC of ACC with 9:19; (E) One PMC of ACC with 10:18; (F) One PMC of ACC with chromosome bridge; (G) One microspore-derived line with 18 chromosomes; (H) One microspore-derived line with 38 chromosomes; (I) One microspore-derived line with 52 chromosomes. Those marked with stars were bivalent.</p

Microspore culture reveals high fitness of <i>B</i>. <i>napus</i>-like gametes in an interspecific hybrid between <i>Brassica napus</i> and <i>B</i>. <i>oleracea</i>

<div><p>The strategies of crossing <i>B</i>. <i>napus</i> with parental species play important role in broadening and improving the genetic basis of <i>B</i>. <i>napus</i> by the introgression of genetic resources from parental species. With these strategies, it is easy to select new types of <i>B</i>. <i>napus</i>, but difficult to select new types of <i>B</i>. <i>rapa</i> or <i>B</i>. <i>oleracea</i> by self-pollination. This characteristic may be a consequence of high competition with <i>B</i>. <i>napus</i> gametes. To verify the role of gamete viability in producing new <i>B</i>. <i>napus</i> individuals, the meiotic chromosome behavior of the interspecific hybrid between <i>B</i>. <i>napus</i> (Zhongshuang 9) and <i>B</i>. <i>oleracea</i> (6m08) was studied, and microspore-derived (MD) individuals were analyzed. The highest fitness of the 9:19 (1.10%) pattern was observed with a 5.49-fold higher than theoretical expectation among the six chromosome segregation patterns in the hybrid. A total of 43 MD lines with more than 14 chromosomes were developed from the hybrid, and 8 (18.6%) of them were <i>B</i>. <i>napus</i>-like (n = 19) type gametes, having the potential to broaden the genetic basis of natural <i>B</i>. <i>napus</i> (GD = 0.43 ± 0.04). It is easy to produce <i>B</i>. <i>napus</i>-like gametes with 19 chromosomes, and these gametes showed high fitness and competition in the microspore-derived lines, suggesting it might be easy to select new types of <i>B</i>. <i>napus</i> from the interspecific hybrid between <i>B</i>. <i>napus</i> and <i>B</i>. <i>oleracea</i>.</p></div

Phylogenetic trees showing the relationships between 30 MD progeny (red lines) and 42 <i>B</i>. <i>oleracea</i> (blue lines), 34 <i>B</i>. <i>napus</i> (green lines).

<p>Phylogenetic trees showing the relationships between 30 MD progeny (red lines) and 42 <i>B</i>. <i>oleracea</i> (blue lines), 34 <i>B</i>. <i>napus</i> (green lines).</p

Comparing the genetic distance of <i>B</i>. <i>napus</i>-like individuals, aneuploid and unreduced gametes of microspore-derived lines to the parental species, natural <i>B</i>. <i>napus</i> and <i>B</i>. <i>oleracea</i>.

<p>Comparing the genetic distance of <i>B</i>. <i>napus</i>-like individuals, aneuploid and unreduced gametes of microspore-derived lines to the parental species, natural <i>B</i>. <i>napus</i> and <i>B</i>. <i>oleracea</i>.</p

Morphology of the ACC interspecific hybrid between <i>B</i>. <i>napus</i> and <i>B</i>. <i>oleracea</i> and its microspore-derived lines.

<p>(A) Zhongshuang 9 seedling; (B) 6m06 seedling; (C) hybrid ACC seedling; (D-I) Seedling of microspore-derived lines from the hybrid between Zhongshuang 9 and 6m08.</p

Copper oxide nanoparticles suppress retinal angiogenesis via inducing endothelial cell cuproptosis - Supplementary material

Supplementary figures 1-5</p

Polymerization-Induced Colloid Assembly Route to Iron Oxide-Based Mesoporous Microspheres for Gas Sensing and Fenton Catalysis

Iron oxide materials have wide applications due to their special physicochemical properties; however, it is a great challenge to synthesize mesoporous iron oxide-based microspheres conveniently and controllably with high surface area, large pore volume, and interconnected porous structures. Herein, mesoporous α-Fe₂O₃-based microspheres with high porosity are synthesized via a facile polymerization induced colloid assembly method through polymerization of urea–formaldehyde resin (UF resin) and its simultaneously cooperative assembly with Fe­(OH)₃ colloids in an aqueous solution, followed by subsequent thermal treatment. Remarkably, by controlling the cross-linking degree of UF, pure mesoporous α-Fe₂O₃ and α-Fe₂O₃/carbon hybrid microspheres can be synthesized controllably, respectively. They exhibit a uniform spherical morphology with a particle size of around 1.0 μm, well-interconnected mesopores (24.5 and 8.9 nm, respectively), and surface area of 54.4 m²/g (pure mFe₂O₃ microspheres) and 144.7 m²/g (hybrids), respectively. As a result, mesoporous pure α-Fe₂O₃ microspheres exhibited excellent H₂S sensing performance with a good selectivity, high response to low concentration H₂S at 100 °C, and quick response (4 s)/recovery (5 s) dynamics owing to the high surface area, open mesopores, and crystalline structure of the n-type α-Fe₂O₃ semiconductor. Moreover, mesoporous α-Fe₂O₃/carbon hybrid microspheres were used as a novel Fenton-like catalyst for the decomposition of methylene blue in a mild condition and exhibit quick degradation rate, high removal efficiency (∼93% within 35 min), and stable recycling performance owing to the synergetic effect of a high surface area and the carbon-protected α-Fe₂O₃

Table_1_Identification of Candidate Genes for Clubroot-Resistance in Brassica oleracea Using Quantitative Trait Loci-Sequencing.XLSX

Clubroot caused by Plasmodiophora brassicae is a devastating disease of cabbage (Brassica oleracea). To identify quantitative trait loci (QTLs) for clubroot resistance (CR) in B. oleracea, genomic resequencing was carried out in two sets of extreme pools, group I and group II, which were constructed separately from 110 and 74 F2 cloned lines derived from the cross between clubroot-resistant (R) cabbage “GZ87” (against race 4) and susceptible (S) cabbage “263.” Based on the QTL-sequencing (QTL-Seq) analysis of group I and group II, three QTLs (i.e., qCRc7-2, qCRc7-3, and qCRc7-4) were determined on the C07 chromosome. RNA-Seq and qRT-PCR were conducted in the extreme pools of group II before and after inoculation, and two potential candidate genes (i.e., Bol037115 and Bol042270), which exhibiting upregulation after inoculation in the R pool but downregulation in the S pool, were identified from the three QTLs on C07. A functional marker “SWU-OA” was developed from qCRc7-4 on C07, exhibiting ∼95% accuracy in identifying CR in 56 F2 lines. Our study will provide valuable information on resistance genes against P. brassicae and may accelerate the breeding process of B. oleracea with CR.</p

Table_6_Identification of Candidate Genes for Clubroot-Resistance in Brassica oleracea Using Quantitative Trait Loci-Sequencing.XLSX

Clubroot caused by Plasmodiophora brassicae is a devastating disease of cabbage (Brassica oleracea). To identify quantitative trait loci (QTLs) for clubroot resistance (CR) in B. oleracea, genomic resequencing was carried out in two sets of extreme pools, group I and group II, which were constructed separately from 110 and 74 F2 cloned lines derived from the cross between clubroot-resistant (R) cabbage “GZ87” (against race 4) and susceptible (S) cabbage “263.” Based on the QTL-sequencing (QTL-Seq) analysis of group I and group II, three QTLs (i.e., qCRc7-2, qCRc7-3, and qCRc7-4) were determined on the C07 chromosome. RNA-Seq and qRT-PCR were conducted in the extreme pools of group II before and after inoculation, and two potential candidate genes (i.e., Bol037115 and Bol042270), which exhibiting upregulation after inoculation in the R pool but downregulation in the S pool, were identified from the three QTLs on C07. A functional marker “SWU-OA” was developed from qCRc7-4 on C07, exhibiting ∼95% accuracy in identifying CR in 56 F2 lines. Our study will provide valuable information on resistance genes against P. brassicae and may accelerate the breeding process of B. oleracea with CR.</p