Flow Induced Dispersion Analysis Quantifies Noncovalent Interactions in Nanoliter Samples

Flow Induced Dispersion Analysis Quantifies Noncovalent Interactions in Nanoliter Sample

Simultaneous Evaluation of Ligand Binding Properties and Protein Size by Electrophoresis and Taylor Dispersion in Capillaries

The interplay between biophysical characteristics such as protein size and shape and protein function is difficult to ascertain using simple methods. Here, we present an approach for characterizing both protein−ligand binding as well as protein hydrodynamic radius in one operation combining electrophoresis and size measurement by dispersion using capillaries. The methodology is based on the integration of Taylor dispersion analysis and capillary electrophoresis and is here demonstrated using commercially available capillary electrophoresis instrumentation modified with a pixel sensor UV area imager, allowing two detection points along the capillary. Analytes are the human serum proteins α1-acid glycoprotein and albumin interacting with the drug propranolol in a frontal analysis mode. Upon introduction of the propranolol−protein sample, voltage is initially applied to facilitate electrophoretically mediated separation of ligand and protein and frontal analysis. Then a pressure mobilization step is used whereby Taylor dispersion can be characterized online based on the signal from the UV area imager. Estimates of ligand binding and values for hydrodynamic radii agree with values obtained by independent methods

Fledglings

The file contains data for analyses of the effect of inbreeding on recruitment probability of house sparrow fledglings

Versatile Flow-Injection Amperometric Ion Detector Based on an Interface between Two Immiscible Electrolyte Solutions: Numerical and Experimental Characterization

The present paper describes a flexible thin layer electrochemical flow cell for ultrasensitive amperometric detection at a supported interface between immiscible electrolyte solutions. Nanomolar detection limits were demonstrated using the cell design, and 3D finite element simulations allowed a detailed characterization of the flow cell. The cell design employed in the present work allowed the sensing oil membrane and the aqueous reference electrode to be placed in close contact, thereby minimizing cell resistance. The adjustable cell volume means that the same cell design can be used for different application with different requirement for detection limits and dynamic range. A disposable membrane was employed which reduces the need for surface cleaning and prevents sample carryover between different applications. For the lowest cell volumes the detection chamber approaches a thin layer electrochemical flow cell detector with a large surface to volume ratio

MOESM2 of A porcine model for pathomorphological age assessment of surgically excised skin wounds

Additional file 2. Anesthesia and reversal of anesthesia for sampling of biopsies

MOESM4 of A porcine model for pathomorphological age assessment of surgically excised skin wounds

Additional file 4. Infiltration of neutrophils and macrophages was scored as: (0) absent; (1) < 10; (2) 10–20; (3) 21–50; (4) 51–100; (5) 101–200; (6) > 200 using a 40× objective and 10× ocular with FN 22 mm in one high power field (HPF) of 0.237 mm2. The scoring was carried out in the HPF with the highest number of neutrophils and macrophages within areas 1–4 in the wounds (Fig. 2a, b). From each wound, only the highest score was registered, i.e. one neutrophil score and one macrophage score per wound. Location of the wound (1–4), wound age, neutrophil score and macrophage score are presented

MOESM1 of A porcine model for pathomorphological age assessment of surgically excised skin wounds

Additional file 1. Sedation and general anesthesia for surgical incision of wounds

MOESM3 of A porcine model for pathomorphological age assessment of surgically excised skin wounds

Additional file 3. Granulation tissue was measured at the center of the wound from the top of the wound bed (not including epidermis) and excluding underlying fat tissue and the fibrous fascia of the muscle

Adults

The file contains life history data for analyses of the effect of inbreeding on fitness components of adult house sparrow