Senescence associated-β-galactosidase (SA-β-gal) activity and differentiation potential of UC-MSCs cultured on WJE-coated plates.

<p>(A) SA-β-gal staining of UC-MSCs cultured on uncoated and WJE-coated plates. Left panel, representative image of SA-β-gal staining, right panel, positive SA-β-gal staining. (B) Osteogenic differentiation of UC-MSCs. Representative image showing alizarin red staining (left). Histograms show staining ratios (right). (C) Adipogenic differentiation of UC-MSCs. Representative image showing Oil Red O staining (left). Histograms show staining ratios (right). Scale bars 20 µm. Data are mean ± S.E. (n = 3). (*<i>P</i><0.05).</p

Characteristics of Wharton's Jelly extracts (WJE).

<p>(A) Micrographs from phase contrast microscopy of WJE-coated plates. Scale bars, 50 µm. (B) Scanning electron microscope images of WJE-coated plates. Scale bars, 20 µm. (C) Protein removed from WJE-coated plates analyzed by electrophoresis. (D) Mass spectra of a 21 kDa protein band by in-gel digestion with porcine trypsin (representative image). MW, molecular weight. S, sample. b, band.</p

Delayed senescence of BM-MSCs cultured on WJE-coated plastes.

<p>(A) Long-term growth curves of BM-MSCs cultured on WJE-coated plates. (B) Positive SA-β-gal staining of BM-MSCs cultured on WJE-coated plates. (C) Osteogenic differentiation of BM-MSCs cultured on WJE-coated plates. (D) Adipogenic differentiation ability of BM-MSCs cultured on WJE-coated plates. (E) Mean telomere lengths of BM-MSCs determined by real-time PCR. Data are mean ± S.E (n = 3) (*<i>P</i><0.05, **<i>P</i><0.01).</p

Relative telomere length expressed as T/S ratios.

<p>(A) Standard curve for T and S was from serial dilutions of DNA (3.20 ng to 0.10 ng) from reference 293T cells. (B) T/S ratios were plotted against passage number to show UC-MSCs distribution. (C) Mean relative telomere lengths of UC-MSCs. S, single-copy gene 36B4 amplification; T, telomere amplification.</p

Identified proteins extracted from WJE by MALDI-TOF-MS.

<p>Number, band number. MW, molecular weight (Dalton).</p

Suppression of intracellular ROS, p53, and p16INK4a/pRb of UC-MSCs cultured on WJE-coated plates.

<p>(A) Levels of intracellular ROS in UC-MSCs cultured on uncoated or WJE-coated plates measured by DCFH-DA (top) staining and confirmed by FACS analysis (bottom). (B) Histograms show relative DCFH-DA intensity. (C) Suppression of p53, p16INK4a/pRb expression in UC-MSCs cultured on WJE-coated plates at 30 PD or 50 PD compared with 10 PD. Histograms show quantitative measurements. Data are mean ± S.E. (n = 3) (*<i>P</i><0.05).</p

Characteristics of long-term cultured UC-MSCs on WJE-coated plates.

<p>(A) Morphological changes of long-term cultured UC-MSCs on WJE-coated plates by microscopy. Scale bars, 20 µm. (B) Proliferation ability of UC-MSCs cultured on WJE-coated plates by MTT assay. (C) Growth curve of long-term cultured UC-MSCs on WJE-coated plates. (D) Flow cytometric analysis of a representative example of UC-MSC (top). Histograms of a representative UC-MSCs culture on WJE-coated plate stained for lineage negative, and positive cell surface markers. Data are mean ± SD (n = 3) (*<i>P</i>>0.05).</p

Additional file 13 of Human umbilical cord mesenchymal stromal cell small extracellular vesicle transfer of microRNA-223-3p to lung epithelial cells attenuates inflammation in acute lung injury in mice

Supplementary Material 1

Additional file 5 of Human umbilical cord mesenchymal stromal cell small extracellular vesicle transfer of microRNA-223-3p to lung epithelial cells attenuates inflammation in acute lung injury in mice

Supplementary Material

Additional file 3 of Human umbilical cord mesenchymal stromal cell small extracellular vesicle transfer of microRNA-223-3p to lung epithelial cells attenuates inflammation in acute lung injury in mice

Supplementary Material