sj-doc-2-onc-10.1177_11795549221105027 – Supplemental material for Diagnostic Ability of Volatile Organic Compounds in Digestive Cancer: A Systematic Review With Meta-Analysis

Supplemental material, sj-doc-2-onc-10.1177_11795549221105027 for Diagnostic Ability of Volatile Organic Compounds in Digestive Cancer: A Systematic Review With Meta-Analysis by Hang Yang, Yi Mou and Bing Hu in Clinical Medicine Insights: Oncology</p

sj-doc-3-onc-10.1177_11795549221105027 – Supplemental material for Diagnostic Ability of Volatile Organic Compounds in Digestive Cancer: A Systematic Review With Meta-Analysis

Supplemental material, sj-doc-3-onc-10.1177_11795549221105027 for Diagnostic Ability of Volatile Organic Compounds in Digestive Cancer: A Systematic Review With Meta-Analysis by Hang Yang, Yi Mou and Bing Hu in Clinical Medicine Insights: Oncology</p

sj-doc-1-onc-10.1177_11795549221105027 – Supplemental material for Diagnostic Ability of Volatile Organic Compounds in Digestive Cancer: A Systematic Review With Meta-Analysis

Supplemental material, sj-doc-1-onc-10.1177_11795549221105027 for Diagnostic Ability of Volatile Organic Compounds in Digestive Cancer: A Systematic Review With Meta-Analysis by Hang Yang, Yi Mou and Bing Hu in Clinical Medicine Insights: Oncology</p

Detection of <i>Escherichia coli</i> Enoyl-ACP Reductase Using Biarsenical-Tetracysteine Motif

Although the tetracysteine (TC) motif has been used as a tag, the binding stability between TC motif and biarsenical reagent against extreme conditions as well as its capacity as a quantitative tag remains not well developed. To reveal these problems, we chose enoyl−acyl carrier protein reductase (FabI), which was involved in the final step of elongation in the bacterial fatty acid biosynthesis, to be tagged by the TC motif. Taking enhanced green fluorescent protein (EGFP) tagged FabI as a control, we investigated the activities of various TC tagged FabIs (N-terminus, C-terminus, or both N- and C-terminus TC motif). The results showed that all the TC tagged FabIs had high enzyme activities while the EGFP tagged FabI exhaustively lost the activity. Beside this, the characteristics of the tag, including labeling stability against extreme conditions, capacity for quantitative analysis, and ability for in-cell labeling, were also investigated. We demonstrated for the first time that the binding between FlAsH reagent and TC motif was stable against high pressure, high field strength, high temperature, and ultrasound. Furthermore, we verified the potential of TC motif for quantitative analysis of target protein by different approaches, including SDS−PAGE, spectrofluorometry (SPF), and capillary zone electrophoresis (CZE)

Table_1_The comparison of largemouth bass (Micropterus salmoides) fed trash fish and formula feeds: Growth, flesh quality and metabolomics.DOCX

This study compared the growth, flesh quality and metabolomics of largemouth bass (Micropterus salmoides) fed trash fish and formula feeds. Trash fish (TF), self-made feed (SF) and commercial feed (CF) were prepared with crude protein levels of 172.2 g/kg, 503.5 g/kg and 504.1 g/kg (666.2 g/kg, 547.3 g/kg and 535.1 g/kg based on dry matter), respectively. Then, the three diets were fed to largemouth bass with an initial body weight of 75.0 ± 0.1 g for 12 weeks. SF and CF groups presented significantly lower feed intake (FI), feed conversion ratio (FCR) and higher protein efficiency ratio (PER) than TF group based on dry matter basis without affecting the weight gain (P < 0.05). The yellowness (b*) in dorsal muscle, flesh heat-insoluble collagen and free flavor amino acids contents in SF group were significantly higher (P < 0.05), while drip loss were significantly lower (P < 0.05) than those of TF group. Compared to TF group, SF and CF groups showed significantly higher flesh polyunsaturated fatty acids (PUFAs), n-3 PUFAs and n-6 PUFAs contents, flesh hardness, shear force and muscle fiber density (P < 0.05), and lower flesh total free amino acids, essential amino acids, muscle fiber diameter, intestine villus height and muscular thickness (P < 0.05). The serum total protein, triglyceride and cholesterol levels in SF group were significantly lower than those in TF and CF groups (P < 0.05). In the muscle metabolomics, 177 differential metabolites were detected between SF and TF groups, which mainly enriched in pathways as biosynthesis of amino acid, histidine metabolism, glycine, serine and threonine metabolism, etc. Conclusively, feeding largemouth bass with formula feeds improved flesh fatty acid profile and flesh texture without negative effects on the growth, but the flesh free amino acids contents were lower than the fish fed trash fish.</p

Table1_Titanium alloys surface integrity of belt grinding considering different machining trajectory direction.DOCX

The interplay of abrasive grains and materials complicates the grinding of titanium alloys by abrasive belts. Notably, the influence relationship of surface generation for complex curved workpieces such as hollow blades needs to be clarified, making precise control of the surface integrity of complex surfaces difficult in abrasive belt grinding applications. This paper thus proposes a trajectory planning method based on the direction of interaction between grinding grains and materials to reveal its influence law on the surface integrity of complex curved surfaces of titanium alloy with unevenly distributed machining allowances. First, a machining trajectory with different angles between the grinding direction and feed direction is proposed. In order to determine the corresponding experimental scheme for titanium alloy hollow blades. Experimental results are used to analyze the influence of different grinding trajectory directions on the surface roughness, residual stress, surface topography, and accuracy of the contours. The results show that different grinding trajectory directions significantly affect the workpiece’s surface integrity. By varying the grinding trajectory direction, it is possible to reduce the surface roughness of titanium alloy workpieces by approximately 40%, increase the surface residual compressive stress by approximately 50%, provide a finer workpiece surface and improve the consistency of the surface texture. This work is expected to guide the efficient and high-quality machining of complex curved parts such as titanium alloy hollow blades.</p

Long-Term Preservation of SARS-CoV‑2 RNA in Silk for Downstream RT-PCR Tests

Positive controls made of viral gene components are essential to validate the performance of diagnostic assays for pathogens like severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, most of them are target-specific, limiting their application spectrum when validating assays beyond their specified targets. The use of an inactivated whole-virus RNA reference standard could be ideal, but RNA is a labile molecule that needs cold chain storage and transportation to preserve its integrity and activity. The cold chain process stretches the already dwindling storage capacities, incurs huge costs, and limits the distribution of reference materials to low-resource settings. To circumvent these issues, we developed an inactivated whole-virus SARS-CoV-2 RNA reference standard and studied its stability in silk fibroin matrices, i.e., silk solution (SS) and silk film (SF). Compared to preservation in nuclease-free water (ddH2O) and SS, SF was more stable and could preserve the SARS-CoV-2 RNA reference standard at room temperature for over 21 weeks (∼6 months) as determined by reverse transcription polymerase chain reaction (RT-PCR). The preserved RNA reference standard in SF was able to assess the limits of detection of four commercial SARS-CoV-2 RT-PCR assays. In addition, SF is compatible with RT-PCR reactions and can be used to preserve a reaction-ready primer and probe mix for RT-PCR at ambient temperatures without affecting their activity. Taken together, these results offer extensive flexibility and a simpler mechanism of preserving RNA reference materials for a long time at ambient temperatures of ≥25 °C, with the possibility of eliminating cold chains during storage and transportation

Ultra-High Energy Storage Performance in BNT-based Ferroelectric Ceramics with Simultaneously Enhanced Polarization and Breakdown Strength

BNT (Bi0.5Na0.5TiO3)-based ferroelectric ceramics have drawn much attention in energy storage applications due to the high saturation polarization and good temperature stability. However, the reduction of Ti4+ caused by the volatilization of Bi and Na elements during high-temperature sintering is a huge problem. A multivalent element (Mn) is adopted in this work to prevent the reduction of Ti4+ and thus enhance the polarization and breakdown strength simultaneously. Various contents of MnO2-doped 0.76Bi0.5Na0.5TiO3–0.04SrZrO3–0.2NaNbO3 (BNTSZNN) ceramics were prepared by the ramp-to-spike sintering method. As the content of MnO2 increases, the reduction of Ti4+ is effectively decreased, inhibiting the degradation of ferroelectricity and decreasing the leakage conductance. As a result, an ultra-high discharge energy density of 7.05 J/cm3 is achieved in the BNTSZNN-0.15MnO2 ceramic at 387 kV/cm. Importantly, the BNTSZNN-0.15MnO2 ceramic shows excellent temperature stability. The change of the discharge energy density between 30 and 160 °C is less than ±4% under the applied field of 120 kV/cm. Additionally, the variation in the capacitance of the BNTSZNN-0.15 MnO2 ceramic is less than ±15% over the temperature range from −58 to 450 °C, with a high room-temperature dielectric permittivity of 1507. All the above characteristics indicate the potential of BNTSZNN-0.15MnO2 as a high-temperature and high-voltage ceramic dielectric

B7 family member H4 induces epithelial-mesenchymal transition and promotes the proliferation, migration and invasion of colorectal cancer cells

Colorectal cancer (CRC) is a common malignancy of the gastrointestinal tract, which has the second highest incidence among gastrointestinal tumors. At present, due to the limitations of current CRC treatment strategies, there is an urgent need for developing more effective therapies. B7 family member H4 (B7-H4) is associated with the progression of a wide spectrum of cancers, but its functional role in CRC is unknown. The purpose of this study is to clarify the role of B7-H4 in CRC and the underlying mechanisms in controlling the progression of CRC. Our data showed that B7-H4 expression in CRC tissues and cell lines was significantly upregulated as compared with normal tissues and normal cell lines. High B7-H4 expression was correlated with a poor prognosis of CRC patients. B7-H4 overexpression promoted the proliferation and invasion of CRC cells, which could be suppressed by Wnt signaling inhibitor. In a mouse xenograft model, silencing B7-H4 suppressed tumor growth and epithelial–mesenchymal transition (EMT) of CRC cells. Collectively, our study demonstrated the oncogenic roles of B7-H4 in regulating the proliferation, EMT as well as the migration of CRC cells through Wnt signaling pathway. The heightened expression of B7-H4 could serve as a prognostic marker for CRC patients.</p

Table_1_Proteomic Analysis of the Hepatopancreas of Chinese Mitten Crabs (Eriocheir sinensis) Fed With a Linoleic Acid or α-Linolenic Acid Diet.XLSX

As representatives of n-6 and n-3 fatty acids, many studies have analyzed the use of soybean oil and linseed oil rich in linoleic acid (18:2n-6, LA) and α-linolenic acid (18:3n-3, LNA) as better substitutes for fish oil. In aquatic animals, different dietary ratios of LA and LNA could have significant effects on growth, lipid metabolism, immune response, and reproduction. To assess the nutritive value of these two fatty acids in Chinese mitten crab (Eriocheir sinensis), we performed transcriptome analysis and label-free quantification proteomic analysis of the hepatopancreas from mitten crabs fed with LA or LNA diet. Parallel reaction monitoring was used to confirm the reliability of the proteomic analysis. A total of 186 proteins were differentially expressed with fold change ≥1.5 or ≤0.666. Among the 186 proteins, 116 were upregulated and 70 were downregulated in the LA than LNA. Most of these proteins participate in cellular process and metabolism process and have molecular functions such as binding and catalytic activity; the cellular component of these proteins are cell, cell part, membrane, and membrane part. A total of 18 proteins were identified to be related to lipid, carbohydrate, and protein metabolism, and they mainly participate in digestive enzyme activities, fatty acid transport, and glycolysis. Our results provide new insights for further investigation into the replacement of fish oil from mitten crabs with vegetable oils and enable us to better understand the different roles and nutrition value of LA and LNA in mitten crabs.</p