Immune-associated molecular occurrence and prognosis predictor of hepatocellular carcinoma: an integrated analysis of GEO datasets

Hepatocellular carcinoma (HCC) is the fifth most common cancer and the second most common cause of cancer-related deaths worldwide. As immune response failure is the main factor in the occurrence and poor prognosis of HCC, our study aimed to develop an immune-associated molecular occurrence and prognosis predictor (IMOPP) of HCC. To that end, we discovered a 4-gene immune-associated gene signature: C–C motif chemokine ligand 14 (CCL14), kallikrein B1 (KLKB1), vasoactive intestinal peptide receptor 1 (VIPR1), and cluster of differentiation 4 (CD4). When tested on three cohorts as an immune-associated molecular occurrence predictor (IMOP), it had high sensitivity, specificity, and area under the receiver operating characteristics curve. When tested as an immune-associated molecular prognosis predictor (IMPP), it stratified the HCC prognosis for overall survival (Kaplan–Meier analysis, log rank P = 0.0016; Cox regression, HR = 1.832, 95% CI = 1.173–2.859, P = 0.008) and disease-free survival (Kaplan-Meier analysis, log rank P = 0.0227). IMPP also significantly correlated with the clinicopathological characteristics of HCC; integrating it with clinicopathological characteristics improved the accuracy of a nomogram for overall survival prediction (C-index: 0.7097 vs. 0.6631). In HCC tumor microenviroments, the proportion of CD8+ T cells significantly differed between IMOP-stratified groups. We conclude that IMOPP can potentially predict the occurrence of HCC in high-risk populations and improve prognostic accuracy by providing new biomarkers for risk stratification. In addition, we believe that the IMOP mechanism may be related to its effect on the proportion of CD8+ T cells in tumor-infiltrating lymphocytes.</p

Data_Sheet_1_Risk factors for agitation in home-cared older adults with dementia: evidence from 640 elders in East China.docx

BackgroundAgitation is common among older adults with dementia, negatively affecting their quality of life and their caregivers’. Since home care remains the dominant approach for older adults, this study investigates the risk factors for agitation in older adults with dementia in China.MethodsWe perform a cross-sectional study of home-cared older adults with dementia in Ningbo, China, using 2020 data. We use a self-made questionnaire to investigate the risks of agitated behavior and its related factors. We perform descriptive, univariate, and regression analyses.FindingsWe address 640 older Chinese adults; 42.8% of the sample exhibits one or more agitated behaviors. We find that basic health issues, such as activities of daily living (ADL), family support issues, such as Zarit Burden Interview (ZBI) scale and Family APGAR Questionnaire (APGAR), and behavioral awareness issues, such as fall and scald, significantly influence the occurrence of agitation behaviors (p InterpretationAgitated behavior in home-cared older adults with dementia are diverse and related to mental state, family support, and behavioral awareness issues. Caregivers, often family members, should be attentive to the needs of dementia patients and take active and effective measures to improve their quality of life. They should be aware of the causes and triggers of agitated behavior and take steps to reduce its occurrence.</p

Construction of high-performance polymer hydrogel composite materials for artificial bionic organs

Hydrogel, with excellent hydrophilicity biocompatibility and stimuli responsive, has been widely used in biomedical and artificial organs fields. However, synthetic hydrogel usually displays poor gel strength, poor toughness and slow speed of absorbing water. Therefore, extensive exploratory research is still required to develop new composite materials for the application of bionic viscera. In this work, we constructed a new kind of composite hydrogel by a facile wet chemistry method to cross-link three kinds of polymer resin, polyvinyl alcohol, carboxymethyl cellulose, and carboxymethyl starch. The conductivity, moisture content and elastic modulus of hydrogel can be controlled by the dosage of potassium salt, sodium salt, water and glycerol. After molding and testing, the electrical conductivity, elastic modulus, and moisture content of the artificial liver and kidney organs were similar to those of the corresponding pig viscera, which is superior to most of the current research on hydrogels. This work provides a facile approach to synthesize highly efficient hydrogel, which accelerates the application of hydrogel in bionic organs.</p

Impacts of AlaAT3 transgenic poplar on rhizosphere soil chemical properties, enzyme activity, bacterial community, and metabolites under two nitrogen conditions

Poplar stands as one of the primary afforestation trees globally. We successfully generated transgenic poplar trees characterized by enhanced biomass under identical nutrient conditions, through the overexpression of the pivotal nitrogen assimilation gene, pxAlaAT3. An environmental risk assessment was conducted for investigate the potential changes in rhizosphere soil associated with these overexpressing lines (OL). The results show that acid phosphatase activity was significantly altered under ammonium in OL compared to the wild-type control (WT), and a similar difference was observed for protease under nitrate. 16SrDNA sequencing indicated no significant divergence in rhizosphere soil microbial community diversity between WT and OL. Metabolomics analysis revealed that the OL caused minimal alterations in the metabolites of the rhizosphere soil, posing no potential harm to the environment. With these findings in mind, we anticipate that overexpressed plants will not adversely impact the surrounding soil environment.</p

Ag Nanowire-Based Omnidirectional Stretchable Sensing Array for Independent Pressure–Strain Detection

As conventional strain measuring can be merely realized in one particular direction by strain sensors and could not meet the demand of flexible skin-based structures (FSSs), an omnidirectional strain-sensing array equipped with an independent measurement of pressure and strain is assembled. Vacuum filtration was applied to fabricate AgNWs/graphene-based sensing materials, and the substrate structure was optimized using a vertical circular cavity to realize pressure measurement. In addition, the isolation of pressure and strain data was controlled by changing the thickness-related stiffness of the substrate. These approaches demonstrate potential ways of solving data coupling and their compatibility with the current multiparameter measurement

Additional file 3: of Comprehensive dissection of transcript and metabolite shifts during seed germination and post-germination stages in poplar

Table S3. IDs of genes in the 24 clusters. (XLSX 162 kb

Additional file 4: of Comprehensive dissection of transcript and metabolite shifts during seed germination and post-germination stages in poplar

Table S4. The relative abundance of transcription factors at different stages. (XLSX 17 kb

Additional file 9: of Comprehensive dissection of transcript and metabolite shifts during seed germination and post-germination stages in poplar

Table S9. Primary metabolism-related gene profiling. (XLSX 22 kb

Additional file 6: of Comprehensive dissection of transcript and metabolite shifts during seed germination and post-germination stages in poplar

Table S6. High-connectivity transcription factors. (XLSX 16 kb

Additional file 10: of Comprehensive dissection of transcript and metabolite shifts during seed germination and post-germination stages in poplar

Table S10. Genes and correlations involved in a targeted correlation network analysis (TCNA). (XLSX 14 kb