7 research outputs found

    Additional file 2: of The phospholipase DDHD1 as a new target in colorectal cancer therapy

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    Figure S1. DDHD1 silencing. To evaluate DDHD1 silencing a. Real-time PCR and b. Western blot analysis were performed on SW480, HCT116, HS5 and HUVEC transfected for 48 or 72 h with scrambled siRNA or DDHD1 siRNA. (TIFF 6629 kb

    Analysis of cell morphology.

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    <p>Cell morphology was analysed by actin staining (n = 3). Actin is shown in green, DAPI in blue. A) Control at day 14. B) HA/Mgn 90/10 at day 14. C) Control at day 21 with applied magnetic field. D) HA/Mgn 90/10 at day 21 with applied magnetic field. Scale bars: A, B) 250 µm. C, D) 100 µm.</p

    SEM characterization of cell seeded scaffolds.

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    <p>Detailed analysis of cell morphology was assessed by scanning electron microscopy. A) Control at day 7. B) HA/Mgn 95/5 at day 7. C) HA/Mgn 90/10 at day 7. D) HA/Mgn 50/50 at day 7. E) Control at day 14 with applied magnetic field. F) HA/Mgn 90/10 at day 14 with applied magnetic field. G) HA/Mgn 95/5 at day 7, vertical overlapping profile of 3 consecutive images. H) Control at day 7. I, J) HA/Mgn 90/10 at day 7. K) HA/Mgn 95/5 at day 7. Scale bars: A–D) 150 µm, E, F) 10 µm, G) 500 µm, H, I) 50 µm, J) 50 µm, K) 20 µm.</p

    Histological evaluation of the <i>in vivo</i> implanted scaffolds.

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    <p>Toluidine Blue, Acid Fucsin and Fast Green staining shows similar histocompatibility for both scaffolds 4 weeks after implantation (n = 6). A, B) Control, C, D) HA/Mgn 90/10. Scale bars: A, C) 1 mm, B, D) 500 µm.</p
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