Multiple testing procedures under confounding

While multiple testing procedures have been the focus of much statistical research, an important facet of the problem is how to deal with possible confounding. Procedures have been developed by authors in genetics and statistics. In this chapter, we relate these proposals. We propose two new multiple testing approaches within this framework. The first combines sensitivity analysis methods with false discovery rate estimation procedures. The second involves construction of shrinkage estimators that utilize the mixture model for multiple testing. The procedures are illustrated with applications to a gene expression profiling experiment in prostate cancer.Comment: Published in at http://dx.doi.org/10.1214/193940307000000176 the IMS Collections (http://www.imstat.org/publications/imscollections.htm) by the Institute of Mathematical Statistics (http://www.imstat.org

Nonparametric and semiparametric inference for models of tumor size and metastasis

There has been some recent work in the statistical literature for modelling the relationship between the size of primary cancers and the occurrences of metastases. While nonparametric methods have been proposed for estimation of the tumor size distribution at which metastatic transition occurs, their asymptotic properties have not been studied. In addition, no testing or regression methods are available so that potential confounders and prognostic factors can be adjusted for. We develop a unified approach to nonparametric and semiparametric analysis of modelling tumor size-metastasis data in this article. An equivalence between the models considered by previous authors with survival data structures. Based on this relationship, we develop nonparametric testing procedures and semiparametric regression methodology of modelling the effect of size of tumor on the probability at which metastatic transitions occur in two situations. Asymptotic properties of these estimators are provided. Procedures that achieve the semiparametric information bound are also considered. The proposed methodology is applied to data from a screening study in lung cancer

Semiparametric methods for the binormal model with multiple biomarkers

Abstract: In diagnostic medicine, there is great interest in developing strategies for combining biomarkers in order to optimize classification accuracy. A popular model that has been used when one biomarker is available is the binormal model. Extension of the model to accommodate multiple biomarkers has not been considered in this literature. Here, we consider a multivariate binormal framework for combining biomarkers using copula functions that leads to a natural multivariate extension of the binormal model. Estimation in this model will be done using rank-based procedures. We also discuss adjustment for covariates in this class of models and provide a simple two-stage estimation procedure that can be fit using standard software packages. Some analytical comparisons between analyses using the proposed model with univariate biomarker analyses are given. In addition, the techniques are applied to simulated data as well as data from two cancer biomarker studies

Semiparametic models and estimation procedures for binormal ROC curves with multiple biomarkers

In diagnostic medicine, there is great interest in developing strategies for combining biomarkers in order to optimize classification accuracy. A popular model that has been used for receiver operating characteristic (ROC) curve modelling when one biomarker is available is the binormal model. Extension of the model to accommodate multiple biomarkers has not been considered in this literature. Here, we consider a multivariate binormal framework for combining biomarkers using copula functions that leads to a natural multivariate extension of the binormal model. Estimation in this model will be done using rank-based procedures. We show that the Van der Waerden rank score coefficient estimation procedure can be used for the multivariate binormal model. We also discuss adjustment for covariates in this class of models. We provide a simple two-stage estimation procedure that can be fit using standard software packages. Asymptotic results of the proposed methods are given. The techniques are applied to data from two cancer biomarker studies

Proportional Hazards Regression for Cancer Studies

There has been some recent work in the statistical literature for modeling the relationship between the size of cancers and probability of detecting metastasis, i.e., aggressive disease. Methods for assessing covariate effects in these studies are limited. In this article, we formulate the problem as assessing covariate effects on a right-censored variable subject to two types of sampling bias. The first is the length-biased sampling that is inherent in screening studies; the second is the two-phase design in which a fraction of tumors are measured. We construct estimation procedures for the proportional hazards model that account for these two sampling issues. In addition, a Nelson–Aalen type estimator is proposed as a summary statistic. Asymptotic results for the regression methodology are provided. The methods are illustrated by application to data from an observational cancer study as well as to simulated data.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66119/1/j.1541-0420.2007.00830.x.pd

Simultaneous estimation procedures and multiple testing: a decision-theoretic framework

There is recent tremendous interest in statistical methods regarding the false discovery rate (FDR). Two classes of literature on this topic exist. In the first, authors have proposed sequential testing procedures that control the false discovery rate. For the second, authors have studied the procedures involving FDR in a univariate mixture model setting. We consider a decision-theoretic approach to the assessment of FDR-based methods. In particular, we attempt to reconcile the current literature on false discovery rate procedures with more classical simultaneous estimation procedures. Formulation of the link will allow us to apply results from decision theory; we can then traverse between the two literatures. In particular, we propose double shrinkage estimators for the location parameter in the multiple testing problem for false discovery rates and provide conditions for obtaining minimaxity. We also describe a double shrinkage estimation procedure for p-values. Simulation studies are used to explore the risk properties of existing statistical methods and the potential gains of shrinkage. We then develop a procedure for calculating double shrinkage estimators from observed data. The procedures are applied to data from a gene expression profiling study in prostate cancer