Schematic overview of the localization of different components shown in Figure 1 and 4.

<p>Left overview of the components resulting of the 15 component rat data analysis, right overview of the components resulting of the 40 component mouse data analysis. Both Panels A show the localization of the slices (1-12) from left to right. Panels B, C and D give a 3D surface rendered overview of the localization of the different components shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0018876#pone-0018876-g001" target="_blank">figure 1</a> & <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0018876#pone-0018876-g004" target="_blank">4</a> (B =  sagital, C = oblique, D =  axial view). Panels E gives this overview overlaid on RARE images. Colourcode: <i>Rat:</i> motorcortex (red), colliculus inferior (light orange), somatosensory cortex (yellow), auditory cortex (green), hippocampus (light blue), striatum (dark blue), retrosplenial (dys)granular cortex (purple), cingulate cortex (pink), visual cortex (grey). <i>Mice:</i> motorcortex (red), piriform cortex (orange), left somatosensory cortex (light yellow), right somatosensory cortex (dark yellow), left auditory cortex (light green), right auditory cortex (dark green), right dorsal hippocampus (light blue), ventral hippocampus (middle blue), retrosplenial (dys)granular cortex (purple), cingulate cortex (pink), left visual cortex (light grey) right visual cortex (dark grey), left medial entorhinal cortex (light brown), right medial entorhinal cortex (dark brown), left lateral entorhinal cortex and (white) right lateral entorhinal cortex (black).</p

Striatal functional connectivity maps resulting from 15 and 40 components ICA (GIFT) of rat rsfMRI.

<p>The figure shows 4 axial slices of mean components, located at the striatum. The spatial colour-coded z-maps of these components are overlaid on the GE-EPI image. A higher z-score (yellow) represents a higher correlation between the time course of that voxel and the mean time course of this component. For the 15 component analysis (A) the striatum was shown confined in only one component but was divided over two components for the 40 component analysis (B & C).</p

Reproducibility between subjects of 40 components ICA (GIFT) of mouse rsfMRI.

<p>Pictures showing animal cumulative score maps for 16 selected ICA mice components (central slice) of the nine mice overlaid on the GE-EPI image. Colour-code: voxels with z-value higher than 1 for five animals (blue), six animals (blue-green), seven animals (orange), eight animals (red), nine animals (pink).</p

Cortical functional connectivity maps resulting from 15 components ICA (GIFT) of mouse rsfMRI.

<p>The figure shows 4 slices of 2 mean components, demonstrating the separation in the left (A) and right cortex (B). The spatial colour-coded z-maps (axial) of these components are overlaid on the GE-EPI image. A higher z-score (yellow) represents a higher correlation between the time course of that voxel and the mean time course of this component.</p

Functional connectivity maps resulting from 40 components ICA (GIFT) of mouse rsfMRI.

<p>The figure shows 4 axial slices of the 16 mean components. The spatial colour-coded z-maps of these components are overlaid on the GE-EPI image. A higher z-score (yellow) represents a higher correlation between the time course of that voxel and the mean time course for this component. Mean components comprise (top-bottom) A) motorcortex, B) piriform cortex, CL) left somatosensory cortex, CR) right somatosensory cortex, DL) left auditory cortex, DR) right auditory cortex, E) right hippocampus (dorsal), F) ventral hippocampus, G) retrosplenial (dys)granular cortex, H) cingulate cortex, IL) left visual cortex, IR) right visual cortex, JL) left medial entorhinal cortex, JR) right medial entorhinal cortex, KL) left lateral entorhinal cortex and KR) right lateral entorhinal cortex.</p

Reproducibility between sessions and between subjects of 15 components ICA (GIFT) of rat rsfMRI.

<p>Left: Pictures showing session cumulative score maps for 9 selected mean ICA rat components (central slice) over the different time points overlaid on the GE-EPI image. Colour-code: voxels with z-value higher than 1 for one time point (blue), two time points (blue-green), three time points (orange), four time points (red). Right: Pictures showing animal cumulative score maps for 9 selected ICA rat components (central slice) of the five rats overlaid on the GE-EPI image. Colour-code: voxels with z-value higher than 1 for one animal (blue), two animals (blue-green), three animals (orange), four animals (red), five animals (pink).</p

Overview of the appearance of the main anatomical regions within each ICA analysis, comparing mouse and rat data for the 15 and 40 component analysis.

<p>Overview of the appearance of the main anatomical regions within each ICA analysis, comparing mouse and rat data for the 15 and 40 component analysis.</p

Functional connectivity maps resulting from 15 components ICA (GIFT) of rat rsfMRI.

<p>The figure shows 4 axial slices covering the anatomical area of each of the 9 mean components. The spatial colour-coded z-maps of these components are overlaid on the GE-EPI image. A higher z-score (yellow) represents a higher correlation between the time course of that voxel and the mean time course of this component. Mean components comprise (top-bottom) A) motorcortex, B) somatosensory cortex, C) auditory cortex, D) retrosplenial (dys)granular cortex, E) hippocampus, F) striatum, G) cingulate cortex, H) visual cortex and I) inferior colliculus; J) example of one slice effect.</p