Correlation between the B7-H1-positive rate and miR-152 and miR-200b levels in human gastric cancer tissue samples.

The percentages of B7-H1-positive cancer cells, as determined by double immunofluoresence, in 20 human gastric cancer samples were plotted against the levels of miR-152 (A) and miR-200b (B), as determined by qPCR. The linear regression was analyzed using SPSS.</p

Binding of miR-152 and miR-200b to B7-H1 mRNA.

<p>(A) Schematic of B7-H1 mRNA showing potential miR-152 and miR-200b binding sites in the 3’-UTR and schematic of the luciferase reporter. The complementary miR-152 and miR-200b binding sites in the B7-H1 3’ UTR were inserted downstream of a luciferase reporter. (B) Targeting of the B7-H1 mRNA 3’-UTR by miR-152 or miR-200b results in translational suppression. * p<0.05.</p

B7-H1 expression in AGS cells after treatment with mimics or inhibitors of miR-152 and miR-200b.

<p>(A) AGS cells were transfected with various miRNA mimics for 48 h, and the cells with surface B7-H1 expression were sorted by flow cytometry. (B) AGS cells were treated with various miRNA inhibitors for 48 h, and the cells containing surface B7-H1 expression were sorted by flow cytometry.</p

Cytometric analysis of AGS cells after HP infection and/or IFN-γ treatment.

<p>B7-H1 expression in AGS cells was determined by flow cytometry after co-culturing the cells with HP (HP/cell ratio: 100/1) and/or treating the cells with IFN-γ (10 ng/ml) for 24 h. (A) Quantification of the cytometric data shows the percentages of B7-H1-positive AGS cells. (B) The ratio of the MFI (mean fluorescence intensity) of each group to the MFI of the isotype group.</p

Levels of miR-152 and miR-200b in gastric cancer cells after HP infection or IFN-γ treatment.

<p>AGS cells were co-cultured with HP (HP/cell ratio: 100/1) and/or treated with IFN-γ (100 ng/ml) for 24 h, followed by qPCR to measure the levels of miR-152 (A) and miR-200b (B) in cell lysates.</p

B7-H1 positive rate in clinical samples.

<p>B7-H1 positive rate in clinical samples.</p

B7-H1 levels in the HP-positive and HP-negative gastric cancer tissue samples.

<p>(A,B) HP+ and HP- gastric cancer tissue samples were double-immunostained with antibodies against Epcam (green) and B7-H1 (red). Hoechst staining (blue) was used to label the nuclei. (C) The ratio of B7-H1-positive cells to Epcam-positive cells in the HP+ (n = 76) and HP- (n = 20) gastric cancer tissue samples.</p