Digital data deposition and Python global fitting scripts for :Quantitative analysis of sterol-modulated monomer-dimer equilibrium of the β1-adrenergic receptor by DEER spectroscopy. Proc. Natl. Acad. Sci. U.S.A. 120, e2221036120 (2023) - doi: 10.1073/pnas.2221036120

1. Raw Deer echo curves in digital format for titrations of spin-labeled β1-adrenergic receptor with cholesteryl hemmisuccinate and sodium cholate 2. Python scripts for global fitting of DEER titration data</p

Data_Huntingtin_Profilin.zip

All experimental CPMG and exchange-induced shift data, as well as Matlab scripts used to globally fit the experimental data, presented in the following paper published in Proc. Natl. Acad. Sci. U.S.A.Ceccon, A., Tugarinov, V., Ghirlando, R and Clore, G.M. (2020) Abbrigation of prenucleation, transient oligomerization of the Huntingtin exon 1 protein by human profilin I. Proc. Natl. Acad. Sci. U.S.A. epub ahead of print doi: http://www.pnas.org/cgi/doi/10.1073/pnas.1922264117</div

Digital data deposition and Matlab fitting scripts for the paper entitled "Quantitative NMR analysis of the mechanism and kinetics of chaperone Hsp104 action on amyloid-b42 aggregation and fibril formation"

1. All Matlab scripts used to fit the data for aggregation of amyloid Ab42 over a wide range of Hsp104 concentrations as monitored by the decay of monomer NMR intensity 2. Average intensities over 21 well-resolved residues as a function of time and Hsp104 concentration 3. Intensities of the individual 21 residues as a function of time and Hsp104 concentration</p

A S/T motif controls reversible oligomerization of the Hsp40 chaperone DNAJB6b through subtle reorganization of a β-sheet backboneUntitled Item

1. 15N_relaxation: This folder contains the relaxation and chemical shift data (as defined in the filenames) used in the fits to the 4 state model for WT and T142A in the corresponding folders. The R2_R1.txt file contains the values of R2 and R1 for the main state A. The fit4state.py file fits the data to the 4 state model (requires the numpy and lmift modules) (currently set up for WT fit) states.py contains the chemical shift, relaxation and exchange matrices for various models2. csp_fits Contains the chemical shift titration data for WT (Figure S11). The concentration of total CTD added is noted in the filename and each file has the format atom1;atom2 chemShift_atom1(Hz) chemShift_atom2(Hz)3. methyl_relaxation 1H and 13C methyl CPMG data. The data are separated in two datasets, termed 'B1' and 'Cterm' for residues in proximity to ST16, ST17 and those in the C-terminus respectively. 1H and 13C exchange induced chemical shifts are provided in the X_ExchShifts_*.txt files The CPMG 3kHz folder contains the 1H, 13C r2eff values at 3kHz cpmg field used to derive r relationships between R2a at 600 MHz and 800 MHz fitCPMG_CS_2state.py performs fits to a 2-state model (requires the numpy and lmift modules) and states.py contains the chemical shift, relaxation and exchange matrices</div

Visualization of sparsely-populated lower-order oligomeric states of human mitochondrial Hsp60 by cryo-electron microscopy

2D class averagesXplor-NIH python scripts used for structure calculationsAdditional contact restraintsCoordinates for mtHsp60 3mer, 4mer, 5mer, open 6mer, closed 6mer and 7mer</div

Digital data Deposition and Matlab fitting scripts for the paper entitled "Quantitative NMR analysis of the kinetics of pre-nucleation oligomerization and aggregation of a pathogenic exon 1-derived huntingtin polypeptide. Proc. Natal. Acad. Sci. U.S.A. 2022, 119:e2207690119"

Experimental data used in "Quantitative NMR analysis of the kinetics of pre-nucleation oligomerization and aggregation of a pathogenic exon 1-derived huntingtin polypeptide" 1. 1HN/15N assignments 2. Concentration dependent 15N-exchange inudced shift 1H-15N cross-peak volume/intensity ratio data including matlab fitting scripts 3. Aggregation data including Matlab fitting scripts.</p