Editor\u27s Notes, Chiba Medical Journal 90-1

Multiple sequence alignment of deduced amino acid sequences of 25 wheat annexin genes with rice annexin OsAnn2 (Os05g31760) obtained by ClustalW. (PDF 212 kb

Additional file 2: of Genome-wide characterization of JASMONATE-ZIM DOMAIN transcription repressors in wheat (Triticum aestivum L.)

Table S1. The primers used in this study. Table S2. The information of JAZ or JAZ-like genes in different plants. Table S3. The number and composition of cis- acting regulatory elementsof each TaJAZ gene. (ZIP 40 kb

Analysis of genes involved in the variation region on different chromosome.

<p>Analysis of genes involved in the variation region on different chromosome.</p

Illumina SNP array results for the distal 15q region.

<p>(A) Proband's mother had a 1.6 Mb microdeletion at the 15q21.3 region (chr15: 57413776–59084268) (Human GRCh37/hg19 Assembly). (B) The Proband had a 35.4 Mb microduplication at the 15q21.3-q26.2 region (chr15: 58914326–94371025) (Human GRCh37/hg19 Assembly).</p

Chromosome G-banding karyotype showing a complex chromosome rearrangement of the proband and her mother.

<p>(A) Aberrant chromosomes 3 and 5 of the proband. (B) CCRs between chromosomes 3, 5, 8, 11 and 15 of the mother. (C). Normal karyotype of the father. Arrows point to derivative chromosomes.</p

Sequencing results of the CCRs of the mother.

<p>Partial karyotype and ideogram of the proband’s mother showed the exceptional CCRs between chromosomes 3, 5, 8, 11 and 15. Reversed arrow indicates chromosome inversion.</p

PCR confirmation of chromosome arrangements of the proband and her parents.

<p>(A) PCR results of chromosome rearrangements of Chr3-15, Chr5r-5, Chr5-5r, Chr15-15 and Chr5-11 of the proband and her mother. The normal father was regarded as a control. (B) The sequence results of the rearrangement chromosomes using the Sanger method. Each three lines represent one chromosome; the first line and the third line show the normal chromosome sequence; and the middle line demonstrates the rearrangement chromosome sequence, according to the specific regions of chromosome rearrangements shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0154574#pone.0154574.g004" target="_blank">Fig 4</a>. Chr3-15: combination of region 1 of chromosome 3 and region 3 of chromosome 15; Chr5r-5: combination of region 2 and region 3 of chromosome 5; Chr5-5r: combination of region 1 and region 2 of chromosome 5; Chr15-15: combination of region 1 and region 4 of chromosome 15; Chr5-11: combination of region 5 of chromosome 11 and region 5 of chromosome 5. F: father, P: proband, M: mother. 5r: reversed region 2 of chromosome 5. Der: derivative sequence of rearrangement chromosome. The red nucleotides represent the specific sites of translocation breakpoints.</p

Representative images from FISH analysis of the proband’s mother.

<p>(A) FISH using the Telomere 3p probe (spectrum green) and the Telomere 3q probe (spectrum red) revealed a green and red signal on der(3), indicating that the bottom of der(3) was the region of the translocation chromosome. (B) FISH using the Telomere 5p probe (spectrum green) and the Telomere 5q probe (spectrum red) revealed the absence of the red signal and presence of the green signal on der(5), as well as the presence of the red signal on der(11), indicating that telomere 5q was translocated to chromosome 11. (C) FISH using the Telomere 8p probe (spectrum green) and the Telomere 8q probe (spectrum red) revealed the absence of the red signal and presence of the green signal on der(8), as well as the presence of the red signal on der(3), indicating a translocation of 8q to chromosome 3. (D) FISH using the Telomere 15q probe (spectrum red) showed the presence of the red signal on der(15), indicating subtelomeric 15q sequences were retained on der(15) with a subterminal deletion on der(15). (E) FISH using probe D5S23 (spectrum green) at 5p15 and the probe EGR1 (spectrum red) at 5q31 revealed the absence of the red signal and presence of the green signal on distal der(5)’ short arm, as well as the presence of the red signal on der(11), indicating an inversion on the distal short arm of der(5)’. (F) FISH using the probe ETO (spectrum green) at 8q22 and the probe AML1 (spectrum red) at 21q22 showed the presence of the red signal on der(8) and the presence of the green signal on 21, indicating a breakpoint on chromosomes 8q22; the 22q regions of 21 were normal.(G)The MLL probe SCN4B (spectrum green) and TREH (spectrum red) at 11q23 showed the presence of the green and red signal on der(5), indicating a reciprocal translocation between chromosomes 11q and 5q. (H) FISH using probe D15Z1 (spectrum aqua) at 15p11.2, SNRPN (spectrum red) at 15q12 and PML (spectrum green) at 15q24.1 showed the presence of the aqua and red signal on der(15), as well as the presence of the green signal on der(3), indicating a reciprocal translocation between chromosomes 15q and 3q. (I) Probes used with FISH for the detection of chromosome breakpoints as well as CCRs. Reversed arrow demonstrates an inversion region.</p

Additional file 13: of Comprehensive analyses of the annexin gene family in wheat

Full set of annexin sequences from wheat (T. aestivum), barely (H.vulgare), T.urartu and A.tauschii. (PDF 208 kb

Additional file 5: Figure S2. of Comprehensive analyses of the annexin gene family in wheat

Phylogenetic analysis and predicted structure of annexin proteins of 9 monocots plant and 7 discots plant. (PDF 484 kb