津田修二:先天性臍帶ヘルニアに就て 正誤表(第11卷 第3號 綜説欄所載)

The primary 2-DE gel maps at least three biological replicates for control, dehydration treatments (18 h, 24 h and 48 h) and rehydration treatment (R24 h) in Longchun 23. (TIF 1707 kb

Raw data

We collected the data from 2004 to 2018 to test the effect of  grazing exclusion and the duration on stability.</p

THz-PCR Based on Resonant Coupling between Middle Infrared and DNA Carbonyl Vibrations

The carbonyl groups of deoxyribonucleotide can resonantly couple with 53 THz middle infrared, which can highly transmit water without ionization-based damage to DNA molecules. Herein, we predict that vibrational coupling with THz irradiation could lower down the hybridization landscape of nucleic acids and thus affect DNA replication. Using polymerase chain reaction (PCR) as a measure, we found that THz shining can reduce the denature temperature of DNA duplexes by about 3 °C, which allows one to conduct PCR at lower temperature, facilitating long-time amplification reaction without losing enzymatic fidelity, i.e., normal PCR should be carried out at denaturing temperature ∼4 °C higher than the melting temperature (Tm), but THz-PCR only requires temperature ∼1 °C higher than Tm due to the nonthermal effect of THz shining. Moreover, the melting time can also be shortened to 1/5 due to the enhanced vibration coupling with 53 THz irradiation. We proposed THz-PCR as an innovated DNA amplification technique with ultrahigh specificity and sensitivity and also successfully demonstrated its advantages in forensic detections

Pressure-Dependent Kinetics of the Reaction between CH₃OO and OH Focusing on the Product Yield of Methyltrioxide (CH₃OOOH)

The reaction kinetics of methyl peroxy radical (CH3OO) and hydroxyl radical (OH) has attracted an increasing level of interest in the past decade, while the branching yields of various product channels are still under debate. In this work, a comprehensive theoretical effort was made to investigate the branching yield of the stabilized methyltrioxide (CH3OOOH, TRIOX) adduct, which has recently been a research focus. Our computed branching ratio of TRIOX at 298 K and 760 Torr is ∼0.04, in agreement with the result of multiplexed photoionization mass spectrometry. We show that the large branching yield obtained in an early theoretical study mainly originated from the collision-induced strong stabilization presented in their simulation. Our findings clarify the controversial product yield results for this important species in recent studies. The computed rate constants over wide temperature and pressure ranges allow better integration of this reaction into global atmospheric models and low-temperature combustion kinetic models

Effects of Olefin Group and Its Position on the Kinetics for Intramolecular H-Shift and HO₂ Elimination of Alkenyl Peroxy Radicals

Two classes of unimolecular reactions of peroxy radicals are key to autoignition, namely, intramolecular H-atom shift (which promotes autoignition) and concerted HO2 elimination (which inhibits autoignition). Olefin groups are prominent functional groups in biodiesel fuels. This paper explores the effects of the presence of an olefin group and its position on the kinetics of unimolecular reactions of peroxy radicals. CBS-QB3 calculations were carried out for 10 selected alkyl- and alkenylperoxy radicals. Transition-state theory was used to determine the temperature dependence of the high-pressure limiting rate constants, and Rice−Ramsperger−Kassel−Marcus/master equation simulations were performed to determine the pressure dependence of selected rate constants. Tunneling effects were computed using the asymmetric Eckart potential. The contribution of internal rotors to partition functions were included by using the hindered-rotor treatment

RT-PCR Analysis of <i>P-oo</i> Transcripts

<p>RNA was extracted from kernel pericarp (20 DAP), reverse transcribed, and PCR-amplified using primers complementary to both <i>p1</i> and <i>p2</i> transcripts. The progenitor allele <i>(P1-rr11)</i> shows amplification of a 605-bp band from <i>p1.</i> The <i>p-ww2</i> and <i>P-oo</i> alleles show amplification of a 522-bp band characteristic of the 5′ region of the <i>p2</i> gene. The <i>p1-ww1112</i> allele has a deletion of <i>p1;</i> the native <i>p2</i> gene is intact in this allele, but is not expressed in kernel pericarp.</p

Deletions by Reversed <i>Ac</i> Ends Transposition Generate Chimerical Genes

<div><p>The solid circle indicates the centromere, the short vertical line indicates the target site, and the other symbols have the same meaning as those in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0020164#pgen-0020164-g001" target="_blank">Figure 1</a>. (For animated version, see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0020164#pgen-0020164-sv001" target="_blank">Video S1</a>).</p><p>(A) Ac transposase (blue oval) binds to the 5′ end of <i>Ac</i> and 3′ end of <i>fAc</i>.</p><p>(B) As in ordinary transposition, the <i>Ac</i> 5′ end and the <i>fAc</i> 3′ end are excised by transposase cleavage, and the sequences flanking the <i>Ac/fAc</i> ends join together to form a ~13-kb circle. The X mark at the junction indicates the transposon footprint.</p><p>(C) The excised transposon ends insert into a site in intron 2 of <i>p2</i>. The <i>Ac</i> 5′ end joins to the distal side of the insertion site to form a circle, and the <i>fAc</i> 3′ end joins to the proximal side of the insertion site to generate a chimeric gene containing exon 1 and exon 2 of <i>p2</i> and exon 3 of <i>p1</i>.</p><p>This study reports the isolation of the progenitor (A) and deletion products (C). Note that the hypothetical structures shown in (B) are transient in nature and would not be amenable to physical isolation.</p></div

Data_Sheet_2_Development and Validation of the Win-Win Scale.zip

Accumulating evidence has shown that win-win is necessary for both individuals and the society. This research, including two studies, aimed to develop and validate a measurement of the win-win scale. In the first study, we screened the items by item analysis and extracted common factors using exploratory factor analysis (EFA), thus determining a total of 25 items in the initial scale consisted of five dimensions including integrity, advancement, altruism, harmoniousness, and coordination. In the second study, we used first- and second-order confirmatory factor analysis (CFA) to test the scale’s construct validity. The results indicated a good fit between the five-factor model and the data. Based on our results, we have formed a win-win scale by keeping 16 items from the original project pool.</p

Data_Sheet_1_Development and Validation of the Win-Win Scale.zip

Accumulating evidence has shown that win-win is necessary for both individuals and the society. This research, including two studies, aimed to develop and validate a measurement of the win-win scale. In the first study, we screened the items by item analysis and extracted common factors using exploratory factor analysis (EFA), thus determining a total of 25 items in the initial scale consisted of five dimensions including integrity, advancement, altruism, harmoniousness, and coordination. In the second study, we used first- and second-order confirmatory factor analysis (CFA) to test the scale’s construct validity. The results indicated a good fit between the five-factor model and the data. Based on our results, we have formed a win-win scale by keeping 16 items from the original project pool.</p

Phenotypes and Gene Structures of <i>P-oo</i> Alleles

<div><p>(A) The kernel pericarp pigmentation phenotypes specified by the indicated alleles.</p><p>(B) Genomic Southern blot. Genomic DNA from plants homozygous for the indicated alleles was cut with KpnI and HindIII, and hybridized with probes 15 or 8B from the <i>p1</i> gene. Lanes marked <i>P-oo32</i> contain approximately twice as much DNA as lanes marked <i>P1-rr11;</i> this DNA overloading enables the detection of the 7.6-kb band in the KpnI 8B blot, but also results in the intense 6.5-kb band in the HindIII 15 blot.</p><p>(C) Restriction map. The solid and gray boxes are exons 1, 2, and 3 (left to right) of <i>p1</i> and <i>p2,</i> respectively. Red triangles indicate <i>Ac</i> or <i>fAc</i> insertions, and the open and solid arrowheads indicate the 3′ and 5′ ends, respectively, of <i>Ac/fAc</i>. Sequences hybridizing with Southern blot probes are indicated by the solid bars above (probe 8B) and below (probe 15) the map. The short horizontal arrows indicate the orientations and approximate position of PCR primers. Primers are identified by numbers below the arrows. The sequence of the junction of each fusion allele is shown here; the black letters indicate <i>p2</i> sequence, while the red letters indicate <i>fAc</i> sequence. K, KpnI; H, HindIII. Lines below the map indicate the restriction fragments produced by digestion with KpnI or HindIII and hybridizing with the indicated probe; asterisks indicate HindIII restriction sites located within <i>Ac</i> or <i>fAc</i> sequences.</p></div