Primers for <i>V. parahaemolyticus.</i>

<p>Primers for <i>V. parahaemolyticus.</i></p

Specificity analysis of the real-time resistance measurement.

<p>A. The real-time resistance curve of <i>V. parahaemolyticus</i> and other interfering bacteria. B. End-point resistance of <i>V. parahaemolyticus</i> and other interfering bacteria after a 60-min LAMP assay. C. Derivative analysis of the real-time resistance measurement.</p

Comparison of real-time resistance measurement and culture identification.

*<p>There is no statistical difference between the two methods (P>0.05).</p

Sensitivity and regression analyses of the real-time resistance measurement.

<p>A. The real-time resistance curve of <i>V. parahaemolyticus</i> in a concentration gradient. B. The derivative analysis of the real-time resistance measurement of <i>V. parahaemolyticus</i>. C. The regression analysis of the real-time resistance measurement of <i>V. parahaemolyticus.</i> Three samples of the same bacterial concentration were measured twice and all values were recorded as mean(n = 6).</p

Scheme of the real-time resistance measurement for <i>V. parahaemolyticus</i>.

<p>Briefly, the lecithin-dependent hemolysin (LDH) gene of <i>V. parahaemolyticus</i> was amplified by LAMP at first. The subsequent two products, DNA and pyrophosphate, both negative ions, were combined with a positive dye (Crystal violet) and positive ions (Mg2+), leading to an increase in the reaction liquid resistance. This resistance was measured in real-time using a electrode, and <i>V. parahaemolyticus</i> concentration was quantitatively detected through a derivative analysis.</p

Additional file 9 of ddRADseq-assisted construction of a high-density SNP genetic map and QTL fine mapping for growth-related traits in the spotted scat (Scatophagus argus)

Additional file 9 : Table S5. QTL clusters for growth-related traits in S. argus

Additional file 6 of ddRADseq-assisted construction of a high-density SNP genetic map and QTL fine mapping for growth-related traits in the spotted scat (Scatophagus argus)

Additional file 6 : Fig. S3. Illustration of male linkage map for S. argus. The map demonstrates the genetic lengths and marker distribution of 3627 SNP loci along the 24 linkage groups (LG1m-LG24m). The linkage groups are displayed by the vertical bars with black lines in each linkage group indicating a marker position. Genetic distance is shown by the vertical scale line with centiMorgans (cM)

Additional file 1 of ddRADseq-assisted construction of a high-density SNP genetic map and QTL fine mapping for growth-related traits in the spotted scat (Scatophagus argus)

Additional file 1 : Table S1. The statistics of eight growth-related traits of S. argus full-sib family. BW: body weight; TL: total length; BL: body length; BH: body height; PD: pre-dorsal length; PA: pre-anal length; HL: head length; CPH: caudal peduncle height; SD: standard deviation; CV: coefficient of variation

Additional file 8 of ddRADseq-assisted construction of a high-density SNP genetic map and QTL fine mapping for growth-related traits in the spotted scat (Scatophagus argus)

Additional file 8 : Fig. S4. Circos diagram representing the syntenic relationships between the consensus map and sex-specific maps of S. argus. (a) syntenic relationships between consensus map (right) and female map (left); (b) syntenic relationships between consensus map (right) and male map (left). Each colored arc represents a marker match between the linkage groups of two maps

Additional file 5 of ddRADseq-assisted construction of a high-density SNP genetic map and QTL fine mapping for growth-related traits in the spotted scat (Scatophagus argus)

Additional file 5 : Fig. S2. Illustration of female linkage map for S. argus. The map demonstrates the genetic lengths and marker distribution of 3512 SNP loci along the 24 linkage groups (LG1f-LG24f). The linkage groups are displayed by the vertical bars with black lines in each linkage group indicating a marker position. Genetic distance is shown by the vertical scale line with centiMorgans (cM)