Table_1_Detection of KRAS mutation using plasma samples in non-small-cell lung cancer: a systematic review and meta-analysis.xlsx

BackgroundThe aim of this study was to investigate the diagnostic accuracy of KRAS mutation detection using plasma sample of patients with non-small cell lung cancer (NSCLC).MethodsDatabases of Pubmed, Embase, Cochrane Library, and Web of Science were searched for studies detecting KRAS mutation in paired tissue and plasma samples of patients with NSCLC. Data were extracted from each eligible study and analyzed using MetaDiSc and STATA.ResultsAfter database searching and screening of the studies with pre-defined criteria, 43 eligible studies were identified and relevant data were extracted. After pooling the accuracy data from 3341 patients, the pooled sensitivity, specificity and diagnostic odds ratio were 71%, 94%, and 59.28, respectively. Area under curve of summary receiver operating characteristic curve was 0.8883. Subgroup analysis revealed that next-generation sequencing outperformed PCR-based techniques in detecting KRAS mutation using plasma sample of patients with NSCLC, with sensitivity, specificity, and diagnostic odds ratio of 73%, 94%, and 82.60, respectively.ConclusionCompared to paired tumor tissue sample, plasma sample showed overall good performance in detecting KRAS mutation in patients with NSCLC, which could serve as good surrogate when tissue samples are not available.</p

High-Accuracy Peptide Mass Fingerprinting Using Peak Intensity Data with Machine Learning

For MALDI-TOF mass spectrometry, we show that the intensity of a peptide–ion peak is directly correlated with its sequence, with the residues M, H, P, R, and L having the most substantial effect on ionization. We developed a machine learning approach that exploits this relationship to significantly improve peptide mass fingerprint (PMF) accuracy based on training data sets from both true-positive and false-positive PMF searches. The model’s cross-validated accuracy in distinguishing real versus false-positive database search results is 91%, rivaling the accuracy of MS/MS-based protein identification

Data_Sheet_1_Methionine and S-Adenosylmethionine Regulate Monascus Pigments Biosynthesis in Monascus purpureus.DOCX

Amino acid metabolism could exert regulatory effects on Monascus pigments (MPs) biosynthesis. In this work, MPs biosynthesis regulated by methionine and S-adenosylmethionine (SAM) was investigated in Monascus purpureus RP2. The results indicated that the addition of methionine in fermentation significantly reduced MPs production by 60–70%, and it induced a higher expression of SAM synthetase Mon2A2272 and consequently led to SAM accumulation. However, the addition of SAM in fermentation promoted MPs production by a maximum of 35%, while over-expression of the gene Mon2A2272 led to a decrease in MPs yield, suggesting that SAM synthetase and SAM were likely to play different regulatory roles in MPs biosynthesis. Furthermore, the gene transcription profile indicated that SAM synthetase expression led to a higher expression of the transcriptional regulatory protein of the MPs biosynthesis gene cluster, while the addition of SAM gave rise to a higher expression of MPs biosynthesis activator and the global regulator LaeA, which probably accounted for changes in MPs production and the mycelium colony morphology of M. purpureus RP2 triggered by methionine and SAM. This work proposed a possible regulation mechanism of MPs biosynthesis by SAM metabolism from methionine. The findings provided a new perspective for a deep understanding of MPs biosynthesis regulation in M. purpureus.</p

Synthesis of Trifluoromethyl Ketones via Tandem Claisen Condensation and Retro-Claisen C–C Bond-Cleavage Reaction

A highly efficient, operationally simple approach to trifluoromethyl ketones has been developed that builds on the use of a tandem process involving Claisen condensation and retro-Claisen C–C bond cleavage reaction. Enolizable alkyl phenyl ketones were found to react readily with ethyl trifuoroacetate under the promotion of NaH to afford trifluoroacetic ester/ketone exchange products, trifluoromethyl ketones, which were quite different from the general Claisen condensation products, β-diketones. This procedure uses readily available starting materials and can be extended to the preparation of perfluoroalkyl ketones in excellent yield

Table_3_Detection of KRAS mutation using plasma samples in non-small-cell lung cancer: a systematic review and meta-analysis.docx

BackgroundThe aim of this study was to investigate the diagnostic accuracy of KRAS mutation detection using plasma sample of patients with non-small cell lung cancer (NSCLC).MethodsDatabases of Pubmed, Embase, Cochrane Library, and Web of Science were searched for studies detecting KRAS mutation in paired tissue and plasma samples of patients with NSCLC. Data were extracted from each eligible study and analyzed using MetaDiSc and STATA.ResultsAfter database searching and screening of the studies with pre-defined criteria, 43 eligible studies were identified and relevant data were extracted. After pooling the accuracy data from 3341 patients, the pooled sensitivity, specificity and diagnostic odds ratio were 71%, 94%, and 59.28, respectively. Area under curve of summary receiver operating characteristic curve was 0.8883. Subgroup analysis revealed that next-generation sequencing outperformed PCR-based techniques in detecting KRAS mutation using plasma sample of patients with NSCLC, with sensitivity, specificity, and diagnostic odds ratio of 73%, 94%, and 82.60, respectively.ConclusionCompared to paired tumor tissue sample, plasma sample showed overall good performance in detecting KRAS mutation in patients with NSCLC, which could serve as good surrogate when tissue samples are not available.</p

Table_2_Detection of KRAS mutation using plasma samples in non-small-cell lung cancer: a systematic review and meta-analysis.doc

BackgroundThe aim of this study was to investigate the diagnostic accuracy of KRAS mutation detection using plasma sample of patients with non-small cell lung cancer (NSCLC).MethodsDatabases of Pubmed, Embase, Cochrane Library, and Web of Science were searched for studies detecting KRAS mutation in paired tissue and plasma samples of patients with NSCLC. Data were extracted from each eligible study and analyzed using MetaDiSc and STATA.ResultsAfter database searching and screening of the studies with pre-defined criteria, 43 eligible studies were identified and relevant data were extracted. After pooling the accuracy data from 3341 patients, the pooled sensitivity, specificity and diagnostic odds ratio were 71%, 94%, and 59.28, respectively. Area under curve of summary receiver operating characteristic curve was 0.8883. Subgroup analysis revealed that next-generation sequencing outperformed PCR-based techniques in detecting KRAS mutation using plasma sample of patients with NSCLC, with sensitivity, specificity, and diagnostic odds ratio of 73%, 94%, and 82.60, respectively.ConclusionCompared to paired tumor tissue sample, plasma sample showed overall good performance in detecting KRAS mutation in patients with NSCLC, which could serve as good surrogate when tissue samples are not available.</p

Hydrothermal Derived LaOF:Ln³⁺ (Ln = Eu, Tb, Sm, Dy, Tm, and/or Ho) Nanocrystals with Multicolor-Tunable Emission Properties

A series of LaOF:Ln³⁺ (Ln = Eu, Tb, Sm, Dy, Tm, and/or Ho) nanocrystals with good dispersion have been successfully prepared by the hydrothermal method followed a heat-treatment process. Under ultraviolet radiation and low-voltage electron beam excitation, the LaOF:Ln³⁺ nanocrystals show the characteristic f-f emissions of Ln³⁺ (Ln = Eu, Tb, Sm, Dy, Tm, or Ho) and give red, blue-green, orange, yellow, blue, and green emission, respectively. Moreover, there exists simultaneous luminescence of Tb³⁺, Eu³⁺, Sm³⁺, Dy³⁺, Tm³⁺, or Ho³⁺ individually when codoping them in the single-phase LaOF host (for example, LaOF:Tb³⁺, Eu³⁺/Sm³⁺; LaOF:Tm³⁺, Dy³⁺/Ho³⁺; LaOF:Tm³⁺, Ho³⁺, Eu³⁺ systems), which is beneficial to tune the emission colors. Under low-voltage electron beam excitation, a variety of colors can be efficiently adjusted by varying the doping ions and the doping concentration, making these materials have potential applications in field-emission display devices. More importantly, the energy transfer from Tm³⁺ to Ho³⁺ in the LaOF:Tm³⁺, Ho³⁺ samples under UV excitation was first investigated and has been demonstrated to be a resonant type via a quadrupole-quadrupole mechanism. The critical distance (<i>R</i>_Tm–Ho) is calculated to be 28.4 Å. In addition, the LaOF:Tb³⁺ and LaOF:Tm³⁺ phosphors exhibit green and blue luminescence with better chromaticity coordinates, color purity, and higher intensity compared with the commercial green phosphor ZnO:Zn and blue phosphor Y₂SiO₅:Ce³⁺ to some extent under low-voltage electron beam excitation

Luminescence and Energy Transfer Properties of Ca₂Ba₃(PO₄)₃Cl and Ca₂Ba₃(PO₄)₃Cl:A (A = Eu²⁺/Ce³⁺/Dy³⁺/Tb³⁺) under UV and Low-Voltage Electron Beam Excitation

Pure Ca₂Ba₃(PO₄)₃Cl and rare earth ion (Eu²⁺/Ce³⁺/Dy³⁺/Tb³⁺) doped Ca₂Ba₃(PO₄)₃Cl phosphors with the apatite structure have been prepared via a Pechini-type sol–gel process. X-ray diffraction (XRD) and structure refinement, photoluminescence (PL) spectra, cathodoluminescence (CL) spectra, absolute quantum yield, as well as lifetimes were utilized to characterize samples. Under UV light excitation, the undoped Ca₂Ba₃(PO₄)₃Cl sample shows broad band photoluminescence centered near 480 nm after being reduced due to the defect structure. Eu²⁺ and Ce³⁺ ion doped Ca₂Ba₃(PO₄)₃Cl samples also show broad 5d → 4f transitions with cyan and blue colors and higher quantum yields (72% for Ca₂Ba₃(PO₄)₃Cl:0.04Eu²⁺; 67% for Ca₂Ba₃(PO₄)₃Cl:0.016Ce³⁺). For Dy³⁺ and Tb³⁺ doped Ca₂Ba₃(PO₄)₃Cl samples, they give strong line emissions coming from 4f → 4f transitions. Moreover, the Ce³⁺ ion can transfer its energy to the Tb³⁺ ion in the Ca₂Ba₃(PO₄)₃Cl host, and the energy transfer mechanism has been demonstrated to be a resonant type, via a dipole–quadrupole interaction. However, under the low voltage electron beam excitation, Tb³⁺ ion doped Ca₂Ba₃(PO₄)₃Cl samples present different luminescence properties compared with their PL spectra, which is ascribed to the different excitation mechanism. On the basis of the good PL and CL properties of the Ca₂Ba₃(PO₄)₃Cl:A (A = Ce³⁺/Eu²⁺/Tb³⁺/Dy³⁺), Ca₂Ba₃(PO₄)₃Cl might be promising for application in solid state lighting and field-emission displays

Multicolor Tuning of Manganese-Doped ZnS Colloidal Nanocrystals

In this paper, we report a facile route which is based on tuning doping concentration of Mn2+ ions in ZnS nanocrystals, to achieve deliberate color modulation from blue to orange-yellow under single-wavelength excitation. X-ray diffraction (XRD), transmission electron microscopy (TEM), as well as photoluminescence (PL) spectra were employed to characterize the obtained samples. In this process, the relative emission intensities of both ZnS host (blue) and Mn2+ dopant (orange-yellow) are sensitive to the Mn2+ doping concentration, due to the energy transfer from ZnS host to Mn2+ dopant. As a result of fine-tuning of these two emission components, white emission can be realized for Mn2+-doped ZnS nanocrystals. Furthermore, the as-synthesized doped nanocrystals possess extremely narrow size distribution and can be readily transferred into aqueous solution for the next potential applications

Uniform Colloidal Alkaline Earth Metal Fluoride Nanocrystals: Nonhydrolytic Synthesis and Luminescence Properties

In this paper, we present a facile and general synthetic route to high-quality alkaline earth metal fluoride (AEF2, AE = Ca, Sr, Ba) nanocrystals and CaF2:Tb3+ nanocrystals based on the thermal decomposition of corresponding trifluoroacetate precursors in hot oleylamine. X-ray diffraction, transmission electron microscopy, thermogravimetric and differential thermal analysis, Fourier transform infrared spectra, photoluminescence spectra, and kinetic decays were employed to characterize the samples. The use of single-source precursors plays an important role in the formation of high-quality AEF2 nanocrystals, and the formation process is demonstrated in detail. The obtained AEF2 nanocrystals are nearly monodisperse in size and highly crystalline, and they can be well dispersed in nonpolar solvents to form stable and clear colloidal solutions, which all display purple emissions under ultraviolet excitation due to the numerous surface defects of nanocrystals with large surface/volume ratios. Furthermore, we demonstrate the feasibility of introducing Tb3+ ions into the CaF2 host via this method, which shows strong green emission corresponding to the characteristic 5D4−7FJ (J = 3, 4, 5, 6) emission of Tb3+ ions, which can be potentially used as labels for biological molecules