Characteristics and Kinematics of Bali Bull Sperms after Thawing Using Tris Soy Lecithin

Soy functions as an extracellular cryoprotectant, which can maintain the integrity of spermatozoa cell membranes with its main content is lecithin. Lecithin from soybeans protects sperm cells from cold stress and reduces the effects of oxidative stress during cryopreservation. This study aimed to analyse the effect of various levels of lecithin diluent on the quality of Bali bull semen during cryopreservation. Semen collection of Bali bull was carried out once a week during four times consecutively using an artificial vagina. The semen was then diluted using the essential ingredient Tris Aminomethan with the addition of powdered soy lecithin; P1 (1 %), P2 (3 %) and P3 (5 %), respectively. Andromed® (K1) as a positive control and Tris without soy lecithin (K2) as a negative control. The parameters observed were motility, progressive motility, kinematics, viability, membrane integrity, and acrosome integrity. The results of this study showed that the dilution of semen with soy lecithin before and after thawing the semen quality was not significantly different (P< 0.05) in motility, viability, plasma membrane integrity, and acrosome integrity. Meanwhile, the kinematics of VAP, VCL, VSL, DAP, DCL, and DSL showed that the average quality increased at P3 compared to K1, K2, P1, and P2, which decreased after thawing or were significantly different (P< 0.05). It can be concluded that Bali bull semen diluted with 3% and 5% of tris soy lecithin produces good characteristics and kinematics, can protect spermatozoa from cold shock

Effect of Vitamin A on Estrous Intensity, Number of Follicles, Follicles Diameter and Pregnancy Rate in Beef Cows

Moringa leaves are used as feed to increase the intensity of estrus. It is assumed that the positive effect on the Moringa leaves is due to the presence of vitamin A. This study aims to see the response of vitamin A as a supplement feed to the reproductive performance of beef cows including estrous intensity, number of follicles, follicle diameter, and pregnancy rate. The research was conducted in September-November 2022 in Lappariaja District, Bone Regency, South Sulawesi Province. 10 cows were used which were divided into 2 groups, each group consisting of 5 cows. Group 1 was the control group without vitamin A and group 2 was supplemented with vitamin A. The data on estrus intensity, number of follicles and pregnancy rate obtained were processed using the Chi-square analysis of 2x2 contingency tables. If the Chi-square test does not meet the requirements, the formula used is the Fisher exact test. While the data on the diameter of the follicles were analyzed by ANOVA test. The results of the research data showed that supplementation of vitamin A had a significant difference (P<0.05) on the intensity of estrous. Meanwhile, the results of the chi-square test on the number of follicles and pregnancy rates (P>0.05) showed that there was no significant difference. Likewise, the ANOVA test on follicle diameter between control cows and vitamin A treated cows did not have a significant difference. Although in this study it did not affect the number of follicles, follicle diameter and pregnancy rate. However, it has an economic effect of 20% on the pregnancy rate in the livestock industry scale