1,140 research outputs found
Experimental habitat fragmentation disrupts nematode infections in Australian skinks
Habitat conversion and fragmentation threaten biodiversity and disrupt species interactions. While parasites are recognized as ecologically important, the impacts of fragmentation on parasitism are poorly understood relative to other species interactions. This lack of understanding is in part due to confounding landscape factors that accompany fragmentation. Fragmentation experiments provide the opportunity to fill this knowledge gap by mechanistically testing how fragmentation affects parasitism while controlling landscape factors. In a large‐scale, long‐term experiment, we asked how fragmentation affects a host–parasite interaction between a skink and a parasitic nematode, which is trophically transmitted via a terrestrial amphipod intermediate host. We expected that previously observed amphipod declines resulting from fragmentation would result in decreased transmission of nematodes to skinks. In agreement, we found that nematodes were absent among skinks in the cleared matrix and that infections in fragments were about one quarter of those in continuous forest. Amphipods found in gut contents of skinks and collected from pitfall traps mirrored this pattern. A structural equation model supported the expectation that fragmentation disrupted this interaction by altering the abundance of amphipods and suggested that other variables are likely also important in mediating this effect. These findings advance understanding of how landscape change affects parasitism.This work was
funded by an NSF Postdoctoral Research Fellowship in Biology
(1309192) to J. Resasco and NSF funding to K. F. Davies
(DEB-0841892
Latent ice recrystallization inhibition activity in nonantifreeze proteins : Ca2+-activated plant lectins and cation-activated antimicrobial peptides
Organisms living in polar regions have evolved a series of antifreeze (glyco) proteins (AFGPs) to enable them to survive by modulating the structure of ice. These proteins have huge potential for use in cellular cryopreservation, ice-resistant surfaces, frozen food, and cryosurgery, but they are limited by their relatively low availability and questions regarding their mode of action. This has triggered the search for biomimetic materials capable of reproducing this function. The identification of new structures and sequences capable of inhibiting ice growth is crucial to aid our understanding of these proteins. Here, we show that plant c-type lectins, which have similar biological function to human c-type lectins (glycan recognition) but no sequence homology to AFPs, display calcium-dependent ice recrystallization inhibition (IRI) activity. This IRI activity can be switched on/off by changing the Ca2+ concentration. To show that more (nonantifreeze) proteins may exist with the potential to display IRI, a second motif was considered, amphipathicity. All known AFPs have defined hydrophobic/hydrophilic domains, rationalizing this choice. The cheap, and widely used, antimicrobial Nisin was found to have cation-dependent IRI activity, controlled by either acid or addition of histidine-binding ions such as zinc or nickel, which promote its amphipathic structure. These results demonstrate a new approach in the identification of antifreeze protein mimetic macromolecules and may help in the development of synthetic mimics of AFPs
The beginning of time? Evidence for catastrophic drought in Baringo in the early nineteenth century
New developments in the collection of palaeo-data over the past two decades have transformed our understanding of climate and environmental history in eastern Africa. This article utilises instrumental and proxy evidence of historical lake-level fluctuations from Baringo and Bogoria, along with other Rift Valley lakes, to document the timing and magnitude of hydroclimate variability at decadal to century time scales since 1750. These data allow us to construct a record of past climate variation not only for the Baringo basin proper, but also across a sizable portion of central and northern Kenya. This record is then set alongside historical evidence, from oral histories gathered amongst the peoples of northern Kenya and the Rift Valley and from contemporary observations recorded by travellers through the region, to offer a reinterpretation of human activity and its relationship to environmental history in the nineteenth century. The results reveal strong evidence of a catastrophic drought in the early nineteenth century, the effects of which radically alters our historical understanding of the character of settlement, mobility and identity within the Baringo–Bogoria basin
Human O-GlcNAcase Uses a Preactivated Boat-skew Substrate Conformation for Catalysis. Evidence from X-ray Crystallography and QM/MM Metadynamics
Human O-linked β-N-acetylglucosaminidase (hOGA) is one of the two enzymes involved in nuclear and cytoplasmic protein O-GlcNAcylation, an essential post-translational modification. The enzyme catalyzes the hydrolysis of the GlcNAc-O-(Ser/Thr) glycosidic bonds via anchimeric assistance through the 2-acetamido group of the GlcNAc sugar. However, the conformational itinerary of the GlcNAc ring during catalysis remains unclear. Here we report the crystal structure of wild type hOGA in complex with a nonhydrolyzable glycopeptide substrate and elucidate the full enzyme catalytic mechanism using QM/MM metadynamics. We show that the enzyme can bind the substrate in either a chair- or a boat-like conformation, but only the latter is catalytically competent, leading to the reaction products via 1,4B/1S3 → [4E]‡ → 4C1 and 4C1 → [4E]‡ → 1,4B/1S3 conformational itineraries for the first and second catalytic reaction steps, respectively. Our results reconcile previous experimental observations for human and bacterial OGA and will aid the development of more effective OGA inhibitors for diseases associated with impaired O-GlcNAcylation
Absorption-Line Probes of Gas and Dust in Galactic Superwinds
We discuss moderate resolution spectra of the NaD absorption-line in a sample
of 32 far-IR-bright starburst galaxies. In 18 cases, the line is produced
primarily by interstellar gas, and in 12 of these it is blueshifted by over 100
km/s relative to the galaxy systemic velocity. The absorption-line profiles in
these outflow sources span the range from near the galaxy systemic velocity to
a maximum blueshift of 400 to 600 km/s. The outflows occur in galaxies
systematically viewed more nearly face-on than the others. We therefore argue
that the absorbing material consists of ambient interstellar gas accelerated
along the minor axis of the galaxy by a hot starburst-driven superwind. The NaD
lines are optically-thick, but indirect arguments imply total Hydrogen column
densities of N_H = few X 10^{21} cm^{-2}. This implies that the superwind is
expelling matter at a rate comparable to the star-formation rate. This
outflowing material is very dusty: we find a strong correlation between the
depth of the NaD profile and the line-of-sight reddening (E(B-V) = 0.3 to 1
over regions several-to-ten kpc in size). The estimated terminal velocities of
superwinds inferred from these data and extant X-ray data are typically 400 to
800 km/s, are independent of the galaxy rotation speed, and are comparable to
(substantially exceed) the escape velocities for (dwarf) galaxies. The
resulting loss of metals can establish the mass-metallicity relation in
spheroids, produce the observed metallicity in the ICM, and enrich a general
IGM to 10 solar metallicity. If the outflowing dust grains survive their
journey into the IGM, their effect on observations of cosmologically-distant
objects is significant.Comment: 65 pages, including 16 figures. ApJ, in pres
Heavy-light mesons with staggered light quarks
We demonstrate the viability of improved staggered light quarks in studies of
heavy-light systems. Our method for constructing heavy-light operators exploits
the close relation between naive and staggered fermions. The new approach is
tested on quenched configurations using several staggered actionsn combined
with nonrelativistic heavy quarks. The B_s meson kinetic mass, the hyperfine
and 1P-1S splittings in B_s, and the decay constant f_{B_s} are calculated and
compared to previous quenched lattice studies. An important technical detail,
Bayesian curve-fitting, is discussed at length.Comment: 38 pages, figures included. v2: Entry in Table IX corrected and other
minor changes, version appearing in Phys. Rev.
Fluctuations of a holographic quantum Hall fluid
We analyze the neutral spectrum of the holographic quantum Hall fluid
described by the D2-D8' model. As expected for a quantum Hall state, we find
the system to be stable and gapped and that, at least over much of the
parameter space, the lowest excitation mode is a magneto-roton. In addition, we
find magneto-rotons in higher modes as well. We show that these magneto-rotons
are direct consequences of level crossings between vector and scalar modes.Comment: 20 pages, 8 figures; v.2 figures improved, 2 figures added, and text
clarified particularly in Sec. 5, to appear in JHE
The SAMI Galaxy Survey: spatially resolving the main sequence of star formation
We present the ∼800 star formation rate maps for the Sydney-AAO Multi-object Integral field spectrograph (SAMI) Galaxy Survey based on H α emission maps, corrected for dust attenuation via the Balmer decrement, that are included in the SAMI Public Data Release 1. We mask out spaxels contaminated by non-stellar emission using the [O III]/H β, [N II]/H α, [S II]/H α, and [O I]/H α line ratios. Using these maps, we examine the global and resolved star-forming main sequences of SAMI galaxies as a function of morphology, environmental density, and stellar mass. Galaxies further below the star-forming main sequence are more likely to have flatter star formation profiles. Early-type galaxies split into two populations with similar stellar masses and central stellar mass surface densities. The main-sequence population has centrally concentrated star formation similar to late-type galaxies, while galaxies >3σ below the main sequence show significantly reduced star formation most strikingly in the nuclear regions. The split populations support a two-step quenching mechanism, wherein halo mass first cuts off the gas supply and remaining gas continues to form stars until the local stellar mass surface density can stabilize the reduced remaining fuel against further star formation. Across all morphologies, galaxies in denser environments show a decreased specific star formation rate from the outside in, supporting an environmental cause for quenching, such as ram-pressure stripping or galaxy interactions.Support for A.M.M. is provided by National Aeronautics and Space Administration (NASA) through Hubble Fellowship grant #HST-HF2-51377 awarded by the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., for NASA, under contract NAS5-26555. S.M.C. acknowledges the support of an Australian Research Council Future Fellowship (FT100100457). S.B. acknowledges funding support from the Australian Research Council through a Future Fellowship (FT140101166). B.C. is the recipient of an Australian Research Council Future Fellowship (FT120100660). C.F. gratefully acknowledges funding provided by the Australian Research Council’s Discovery Projects (grants DP150104329 and DP170100603). M.S.O. acknowledges the funding support from the Australian Research Council through a Future Fellowship (FT140100255). R.Mc.D. is the recipient of an
Australian Research Council Future Fellowship (project number
FT150100333). N.S. acknowledges support of a University of Sydney
Postdoctoral Research Fellowship. J.v.d.S. is funded under Bland-Hawthorn’s Australian Research Council (ARC) Laureate Fellowship (FL140100278)
DHODH modulates transcriptional elongation in the neural crest and melanoma
Melanoma is a tumour of transformed melanocytes, which are originally derived from the embryonic neural crest. It is unknown to what extent the programs that regulate neural crest development interact with mutations in the BRAF oncogene, which is the most commonly mutated gene in human melanoma1. We have used zebrafish embryos to identify the initiating transcriptional events that occur on activation of human BRAF(V600E) (which encodes an amino acid substitution mutant of BRAF) in the neural crest lineage. Zebrafish embryos that are transgenic for mitfa:BRAF(V600E) and lack p53 (also known as tp53) have a gene signature that is enriched for markers of multipotent neural crest cells, and neural crest progenitors from these embryos fail to terminally differentiate. To determine whether these early transcriptional events are important for melanoma pathogenesis, we performed a chemical genetic screen to identify small-molecule suppressors of the neural crest lineage, which were then tested for their effects on melanoma. One class of compound, inhibitors of dihydroorotate dehydrogenase (DHODH), for example leflunomide, led to an almost complete abrogation of neural crest development in zebrafish and to a reduction in the self-renewal of mammalian neural crest stem cells. Leflunomide exerts these effects by inhibiting the transcriptional elongation of genes that are required for neural crest development and melanoma growth. When used alone or in combination with a specific inhibitor of the BRAF(V600E) oncogene, DHODH inhibition led to a marked decrease in melanoma growth both in vitro and in mouse xenograft studies. Taken together, these studies highlight developmental pathways in neural crest cells that have a direct bearing on melanoma formation
Roadmaps to Utopia: Tales of the Smart City
Notions of the Smart City are pervasive in urban development discourses. Various frameworks for the development of smart cities, often conceptualized as roadmaps, make a number of implicit claims about how smart city projects proceed but the legitimacy of those claims is unclear. This paper begins to address this gap in knowledge. We explore the development of a smart transport application, MotionMap, in the context of a £16M smart city programme taking place in Milton Keynes, UK. We examine how the idealized smart city narrative was locally inflected, and discuss the differences between the narrative and the processes and outcomes observed in Milton Keynes. The research shows that the vision of data-driven efficiency outlined in the roadmaps is not universally compelling, and that different approaches to the sensing and optimization of urban flows have potential for empowering or disempowering different actors. Roadmaps tend to emphasize the importance of delivering quick practical results. However, the benefits observed in Milton Keynes did not come from quick technical fixes but from a smart city narrative that reinforced existing city branding, mobilizing a growing network of actors towards the development of a smart region. Further research is needed to investigate this and other smart city developments, the significance of different smart city narratives, and how power relationships are reinforced and constructed through them
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