23 research outputs found

    Melt curve analysis of the HIV-1 and β-actin biplex reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay.

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    <p>Melt analysis showed products specific to HIV-1 at 78°C and β-actin at 89°C as seen in the singleplex assays. Specificity was confirmed in the presence of normal human plasma (NHP) without HIV-1 or β-actin (negative control) and showed no amplification (n = 3).</p

    A comparison of the effects on the limit of detection (LOD) of assays heated by the non-instrumented nucleic acid amplification (NINA) heater.

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    <p>Combination of assays run with and without warm-up ramp and with or without oil are evaluated. Table entries show the number of replicates that returned a positive result as the numerator of a fraction showing the total number of replicates run in the denominator.</p><p>A comparison of the effects on the limit of detection (LOD) of assays heated by the non-instrumented nucleic acid amplification (NINA) heater.</p

    HIV LAMP amplicon detection via Milenia test strips.

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    <p>(A) Milenia strips used for the detection of fluorescein isothiocyanate (FITC)/biotin-labeled amplicons. Image to the right shows a positive result while image to the left is a negative result. (B) Best cassettes for the dual detection of FITC/biotin (HIV assay) and digoxigenin (DIG)/biotin (β-actin assay)–labeled amplicons. Image shows a positive result for both FITC/biotin amplicons and DIG/biotin amplicons.</p
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