MOESM1 of Spatially resolved transcriptomics reveals plant host responses to pathogens

Additional file 1. Comparison of GaST-seq with Illumina TruSeq, qRT-PCR validation of DE gene expression, wounding time-series GO enrichment, Albugo laibachii spatial transcriptomics experiment, all wet-lab protocols of the GaST-seq workflow

MOESM3 of Spatially resolved transcriptomics reveals plant host responses to pathogens

Additional file 3. GO-term enrichments of spatial affinity propagation clustering

MOESM2 of Spatially resolved transcriptomics reveals plant host responses to pathogens

Additional file 2. Read numbers, ENA accessions, DE genes, GO accessions, clustering information, FLARE associations of experiments

Additional file 2 of Nanopore adaptive sampling: a tool for enrichment of low abundance species in metagenomic samples

Additional file 2. DNA quantification. Figures S1-S17 showing TapeStation and Femto Pulse traces for bacterial DNA extractions

Additional file 1 of Nanopore adaptive sampling: a tool for enrichment of low abundance species in metagenomic samples

Additional file 1. Alignment statistics. Excel spreadsheets showing detailed calculations of alignment statistics

Additional file 3 of Nanopore adaptive sampling: a tool for enrichment of low abundance species in metagenomic samples

Additional file 3. Review histor

PyroCleaning results for mtDNA COI amplicons generated from community samples of Collembola from two forest sites on the island of Tenerife.

<p>Each site has been amplified in triplicate. 62,825 raw reads were generated on 1/2 of a 454 plate from a total of 103,850 raw reads (the remainder of raw reads belonged to another experiment). Raw reads had a maximum length of 521 bp and an average length of 343 bp. Steps 3–6 are summarised in the text. Numbers in brackets represents sequence reduction as the % of raw reads above a minimum length of 170 nucleotides.</p

PyroCleaning results for mtDNA COI amplicons generated from experimental pools constructed from 27 genomic extracts from 23 Collembola species.

<p>Each pool has been amplified in triplicate. Sequences were generated on 1/2 a 454 plate that generated a total of 156,315 raw reads. Raw reads had a maximum length of 534 bp and an average length of 343.4 bp. Steps 3–4 are summarised in the text. Numbers in brackets represents sequence reduction as the % of raw reads above a minimum length of 170 nucleotides.</p

Datasets

This .zip files contains two folders. The folder "Control" contains the 15 raw 454 sequence files generated from the "Test Pools" analysis detailed in Table 2 of the manuscript, and a file of 27 Sanger sequences listed in Table S1. The folder "Tenerife" contains the 6 raw 454 sequence files associated generated from the "Tenerife Forest Samples" analysis detailed in Table 3 of the manuscript

Regression analyses of the percentage of collembolan mtDNA COI sequences within an MID tag pool (<i>y</i> axis) against the percentage of genomic template from which they are derived within an experimental genomic pool (<i>x</i> axis).

<p>Data come from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057615#pone.0057615.s003" target="_blank">Table S1</a>. The panels from top to bottom are MID1 against pool 1, MID4 against pool 2, and MID7 against pool 3.</p