SINGLE RADIAL IMMUNE HEMOLYSIS TEST FOR DETECTION OF ESCHERICHIA-COLI THERMOLABILE ENTERO-TOXIN

14547347

RELATIONSHIP AMONG ENTERO-TOXIGENIC PHENOTYPES, SEROTYPES, AND SOURCES OF STRAINS IN ENTERO-TOXIGENIC ESCHERICHIA-COLI

281242

Genes coding for enterotoxins and verotoxins in porcine Escherichia coli strains belonging to different O:K:H serotypes: Relationship with toxic phenotypes

Seventy-four E. coli strains isolated from piglets with diarrhea or edema disease in Spain were serotyped and examined for production of heat-labile (LT) and heat-stable (ST) enterotoxins (LT-I, LT-II, STaH, STaP, and STb) and verotoxins (VT1, VT2, and VT2v VTe) by phenotypic (Vero cell assay and infant mouse test) and genotypic (colony hybridization and PCR) methods. In general, an excellent correlation was found between the results obtained with a PCR approach and those determined with biological assays. DNA probes used in the hybridization also showed a very good agreement with phenotypic results, with the exception of a VT1 probe that initially produced 10 false-positive reactions. The gene coding for STb (58 strains) was the most prevalent gene detected by PCR, followed by those coding for STa (46 strains), LT (19 strains), VT2v (11 strains), and VT1 (1 strain). Apparently, in Spain three seropathotypes are predominant: (i) 0149:K91:H10 K88(+) LT-I+ STb+ (ii) 0141:K85ab:H- P987(+) STaP+, and (iii) 0138:K81:H14 or H-STaP+ VT2v(+). We conclude that PCR is a fast, specific, and practical method for the identification of enterotoxin and VT genes in clinical and epidemiological studies.35112958296

INDIRECT HEMAGGLUTINATION TEST FOR THE DETECTION OF THERMOLABILE (LT) ENTEROTOXIN FROM ESCHERICHIA-COLI

175425126

EVALUATION OF THE MODIFIED ELEK TEST IN THE PREPARATION OF SPECIMENS FOR THE DETECTION OF THERMOSTABLE ENTEROTOXINS (STA) FROM ESCHERICHIA-COLI

16322422

Receptors on chicken erythrocytes for F42 fimbriae of Escherichia coli isolated from pigs

Haemagglutination of purified F42 fimbriae was found to be inhibited by N-acetyl-galactosamine. Purified F42 fimbrial adhesin reacted with distinct membrane components from chicken erythrocytes (35, 37 and 40 kDa) in immunoblot analysis, suggesting that the binding occurred to proteins or glycoproteins.286338338

PURIFICATION STUDY THERMOSTABLE (ST) ENTERO-TOXIN OF ESCHERICHIA-COLI

13435936

TRANSPOSON MUTAGENESIS AND MEMBRANE-PROTEIN STUDIES IN AN AVIAN COLISEPTICAEMIC ESCHERICHIA-COLI STRAIN

The pathogenicity, antibiotic resistance, plasmid DNA and membrane protein profiles of a colisepticaemic Escherichia coli strain (362) isolated from chickens was studied. It was verified that this strain harboured at least five plasmids. One 88.0 MD plasmid is non conjugative and is responsible for the production of colicin V and serum resistance. The 43.0 MD plasmid is responsible for resistance to bactericidal activity of serum and ampicilin. Curing of these plasmids did not decrease the pathogenicity of the strain. Transposon mutagenesis (Tnpho A) of strain 362 yielded a non-pathogenic derivative strain which had lost a 40.7 kD membrane protein, which is not correlated to the aerobactin and enterochelin systems. We suggest that this protein subunit is involved in the pathogenicity process of avian septicaemic E. coli strains.17191