sj-pdf-1-imr-10.1177_03000605221129102 - Supplemental material for A rare case of pancreatic macrocystic serous cystadenoma in an adolescent: a case report and literature review

Supplemental material, sj-pdf-1-imr-10.1177_03000605221129102 for A rare case of pancreatic macrocystic serous cystadenoma in an adolescent: a case report and literature review by Yu-Jui Chang, Hung-Chang Lee, Chun-Yan Yeung, Wai-Tao Chen and Chuen-Bin Jiang in Journal of International Medical Research</p

A: Representative histological sections of jejunum showing the goblet cells with haematoxylin and eosin stain in mice on day 5 challenged with 5-FU (IP). They were fed with probiotics (<i>Lcr35</i> or <i>LaBi</i>) or saline. The arrows indicated goblet cells. The image acquisition phase was done with a 20x magnification objective. Scale bar = 50μm. B: Jejunal goblet cells after staining were counted. Values were represented as mean ± SEM and were analyzed using one-way ANOVA.

<p>A: Representative histological sections of jejunum showing the goblet cells with haematoxylin and eosin stain in mice on day 5 challenged with 5-FU (IP). They were fed with probiotics (<i>Lcr35</i> or <i>LaBi</i>) or saline. The arrows indicated goblet cells. The image acquisition phase was done with a 20x magnification objective. Scale bar = 50μm. B: Jejunal goblet cells after staining were counted. Values were represented as mean ± SEM and were analyzed using one-way ANOVA.</p

Daily body weight change in percentage of saline or 5-FU-injected mice with/without probiotics (<i>Lcr35</i> or <i>LaBi</i>) administration.

<p>The mice were weighted daily and the results of all groups were compared with those in 5-FU-saline groups for 5 days. In the control groups, the mice were injected saline and administrated with saline (○), <i>Lcr35</i> (□) and <i>LaBi</i> (△). In the experimental groups, the mice were injected 5-FU and administrated with saline (●), <i>Lcr35</i> (■) and <i>LaBi</i> (▲). Data of starting bodyweight are expressed 100% from day 0.</p

Serum levels of TNF-α, IL-6 and IFN-γ by ELISA assays from mice challenged by 5-FU-induced intestinal mucositis on the 5^th day.

<p>They were fed with (+) or without (−) probiotics (<i>Lcr35/LaBi</i>). The data with different superscripted letters are significantly different based on the one-way ANOVA.</p

Patients in LVD group had higher ARFI values than those in HVD group (1.52m/s vs. 1.13m/s, <i>p</i> = 0.003), indicating patients with lower serum 25-OHVD levels had more severe liver fibrosis.

Abbreviations: LVD: lower vitamin D level; ARFI: Acoustic radiation force impulse; HVD: higher vitamin D level; 25-OHVD: 25-hydroxyvitamin D.</p

Serum 25-OHVD, ARFI values, and PedsQL scores of PKBA patients.

Serum 25-OHVD, ARFI values, and PedsQL scores of PKBA patients.</p

A: Representative histology of jejunum showing villus height and crypt depth with haematoxylin and eosin stain in mice on day 5 challenged with 5-FU (IP). They were fed with probiotics (<i>Lcr35</i> or <i>LaBi</i>) or saline. The image acquisition phase was done with a 20x magnification objective. Scale bar = 200μm. B: Values were represented as mean ± SEM and were analyzed using one-way ANOVA. Segments of jejunum were taken for measurement of villus height, crypt depth and villus/crypt ratio per mouse.

<p>A: Representative histology of jejunum showing villus height and crypt depth with haematoxylin and eosin stain in mice on day 5 challenged with 5-FU (IP). They were fed with probiotics (<i>Lcr35</i> or <i>LaBi</i>) or saline. The image acquisition phase was done with a 20x magnification objective. Scale bar = 200μm. B: Values were represented as mean ± SEM and were analyzed using one-way ANOVA. Segments of jejunum were taken for measurement of villus height, crypt depth and villus/crypt ratio per mouse.</p

Different cutoff values of 25-OHVD and corresponding <i>p</i>-values when comparing the medians of ARFI of the two groups.

Different cutoff values of 25-OHVD and corresponding p-values when comparing the medians of ARFI of the two groups.</p

Diarrhea score after administrating probiotics (<i>Lcr35</i> or <i>LaBi</i>) with/without 5-FU treatment.

<p>The mice were recorded daily and the results of all groups were compared with those in 5-FU + saline group for 5 days. In the control groups, the mice injected saline and administrated with saline (○), <i>Lcr35</i> (□) and <i>LaBi</i> (△). In the experimental groups, the mice injected 5-FU and administrated with saline (●), <i>Lcr</i> (■) and <i>LaBi</i> (▲). The severity of diarrhea was attenuated in those mice treated with probiotics in the 5-FU groups. The data with different superscripted letters are significantly different based on the one-way ANOVA.</p

Up-regulations of IL-6, IL-1β and TNF-α in mucositis mice were followed after injection with 5-FU.

<p>Mucositis mice were fed with (+) or without (−) probiotics. Gene expressions of IL-6, IL-1β and TNF-α were determined by Q-PCR (A) jejunum tissue (B) colon tissue. Induction of cytokine expressions were presented as RQ compared to 18sRNA housekeeping gene expression. The data with different superscripted letters are significantly different based on the one-way ANOVA.</p