Transparency in planning, warranting and interpreting research

409 PSI DMRs in the DCL3a RNAi line, and 88 PSI DMRs in the DCL3a RNAi line, associated with significant changes in nearby gene expressio

The CN-OBEE dataset, occupant behavior, thermal environment, and appliance electricity use of a single-family apartment in China

Household is the basic unit of a residential community or building. High-resolution, long-term open data are necessary to help study residential electricity consumption, smart home technologies, and electricity flexibility technologies at a household level. The dataset provided, namely CN-OBEE, includes the per-minute occupant behavior, thermal environment, and appliance electricity use data from a single-family apartment in Beijing, China, for an entire year (from May 31, 2021, to May 31, 2022) and the hourly meteorological data collected by the nearest national weather station for the same period. The household data are collected by an IoT-based data collection platform (IDCP) composed of gateways, sensors, and cloud servers, which can simultaneously collect the occupant presence, indoor environment, window-opening state, and appliance electricity consumption data. The dataset is the first detailed occupant behavior and electricity use dataset of Chinese homes publicly available. As one of the regional features, it includes window-opening behavior and the usage of split air conditioners. It can support electricity load shape analysis, electric power prediction, occupant behavior modeling and validation, building energy modeling and validation, thermal comfort analysis, suitability of energy-related measures, and other types of research for residential buildings at a household level

Code

The vignette explaining and demonstrating the code

Quercetin and allopurinol inhibit renal NALP3 inflammasome activation in streptozotocin (STZ)-treated rats.

<p>Representative Western blot results (A) and graphic presentation showed renal protein expression of rNALP3 (B), rASC (C) and rCaspase-1 (D) in different groups of rats as indicated. Relative protein levels of rNALP3, rASC and rCaspase-1 were determined after normalization with rGAPDH. For rCaspase-1, the active subunit of P20 was detected. The data are expressed as the means ± SEM (n = 3–4). ⁺<i>P</i><0.05, ⁺⁺⁺<i>P</i><0.001 <i>versus</i> normal control; *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 <i>versus</i> STZ control. Graphic presentation of renal mRNA levels by real-time PCR analysis of rNALP3 (E), rASC (F) and rCaspase-1 (G) at 7 weeks after STZ injection in different groups of rats as indicated. The relative mRNA levels were determined after normalization with rGAPDH. The data are expressed as the means ± SEM (n = 3–4). ⁺⁺⁺<i>P</i><0.001 <i>versus</i> normal control; *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 <i>versus</i> STZ control. Representative Western blot results (H) and graphic presentation showed renal maturation ratio of IL-1β (I) and IL-18 (J) in different groups of rats as indicated. The data are expressed as the means ± SEM (n = 3–4). ⁺<i>P</i><0.05, ⁺⁺<i>P</i><0.01 <i>versus</i> normal control; *<i>P</i><0.05, **<i>P</i><0.01 <i>versus</i> STZ control.</p

The primers used for Real-Time PCR.

<p>The primers used for Real-Time PCR.</p

Quercetin and allopurinol restore streptozotocin (STZ)-induced hyperuricemia and kidney dysfunction in rats.

<p>Biochemical analyses showed uric acid (A-C), creatinine (D-F) and urea nitrogen (G-I) levels in serum and urine, as well as their clearance rates at 7 weeks after STZ injection in different groups of rats as indicated. The data are expressed as the means ± SEM (n = 8). ⁺⁺⁺<i>P</i><0.001 <i>versus</i> normal control; *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 <i>versus</i> STZ control.</p

Quercetin and allopurinol regulate renal mRNA levels of lipid metabolism-related genes in streptozotocin (STZ)-treated rats.

<p>Graphic presentation of renal mRNA levels by real-time PCR analysis of rPPAR-α (A), rCPT1 (B), rACC2 (C) and rOCTN2 (D) at 7 weeks after STZ injection in different groups of rats as indicated. The relative mRNA levels were determined after normalization with rGAPDH. The data are expressed as the means ± SEM (n = 3–4). ⁺⁺⁺<i>P</i><0.001 <i>versus</i> normal control; *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 <i>versus</i> STZ control.</p

Quercetin and allopurinol regulate renal L-carnitine levels in streptozotocin (STZ)-treated rats.

<p>Biochemical analyses showed L-carnitine levels in serum (A) and urine (B) and kidney (C) at 7 weeks after STZ injection in different groups of rats as indicated. The data are expressed as the means ± SEM (n = 8). ⁺⁺⁺<i>P</i><0.001 <i>versus</i> normal control; *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 <i>versus</i> STZ control.</p

Quercetin and allopurinol regulate renal urate transport-related proteins in streptozotocin (STZ)-treated rats.

<p>Representative Western blot results (A) and graphic presentation showed renal protein expression of rOAT1 (B), rOAT3 (C), rUAT (D), rGLUT9 (E) and rRST (F) at 7 weeks after STZ injection in different groups of rats as indicated. The relative protein levels of rOAT1, rOAT3, rUAT and rGLUT9 were determined after normalization with rGAPDH. The relative BBMV rRST protein levels were normalized to rNa⁺-K⁺-ATPase. The data are expressed as the means ± SEM (n = 3–4). ⁺⁺<i>P</i><0.01, ⁺⁺⁺<i>P</i><0.001 <i>versus</i> normal control; *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 <i>versus</i> STZ control.</p

Presentation_1.PDF

<p>Background: Evidence supports that the hypofunction of N-methyl-D-aspartate receptor (NMDAR) and downregulation of disrupted-in-schizophrenia 1 (DISC1) contribute to the pathophysiology of schizophrenia. N-Methyl D-aspartate receptor subtype 2B (NR2B)-containing NMDAR are associated with cognitive dysfunction in schizophrenia. GLYX-13 is an NMDAR glycine-site functional partial agonist and cognitive enhancer that does not induce psychotomimetic side effects. However, it remains unclear whether NR2B plays a critical role in the GLYX-13-induced alleviation of schizophrenia-like behaviors in mice.</p><p>Methods: The effect of GLYX-13 was tested by observing changes in locomotor activity, novel object recognition ability, and prepulse inhibition (PPI) induced by dizocilpine (known as MK-801) in mice. Lentivirus-mediated NR2B knockdown in the hippocampus was assessed to confirm the role of NR2B in GLYX-13 pathophysiology, using Western blots and immunohistochemistry.</p><p>Results: The systemic administration of GLYX-13 (0.5 and 1 mg/kg, i.p.) ameliorates MK-801 (0.5 mg/kg, i.p.)-induced hyperlocomotion, deficits in memory, and PPI in mice. Additionally, GLYX-13 normalized the MK-801-induced alterations in signaling molecules, including NR2B and DISC1 in the hippocampus. Furthermore, we found that NR2B knockdown produced memory and PPI deficits without any changes in locomotor activity. Notably, DISC1 levels significantly decreased by NR2B knockdown. However, the effective dose of GLYX-13 did not alleviate the memory and PPI dysfunctions or downregulation of DISC1 induced by NR2B knockdown.</p><p>Conclusion: Our results suggest GLYX-13 as a candidate for schizophrenia treatment, and NR2B and DISC1 in the hippocampus may account for the molecular mechanisms of GLYX-13.</p