Detection of African Swine Fever Virus Antibodies in Serum and Oral Fluid Specimens Using a Recombinant Protein 30 (p30) Dual Matrix Indirect ELISA - Fig 2

ASFV recombinant p30 antibody ELISA sample-to-positive (S/P) responses in (2A) individual pig serum (mean, SE) and (2B) pen-based oral fluid samples following inoculation with replicon particle (RP) vaccines expressing ASFV p30 or a combination of ASFV p30/54/72. Controls were defined as unvaccinated pigs (one pen of 4 pigs) and pigs inoculated with RP vaccines expressing ASFV p54 (one pen of 8 pigs) or ASFV p72 (one pen of 8 pigs). In all cases, pigs received two doses of vaccine (days 0 and 21).</p

ASFV multiplex fluorescent microbead-based immunoassay (FMIA) sample-to-positive (S/P) serum antibody response (mean, SE) against three recombinant antigens (p30, p54, p72) in 9 pigs inoculated with ASFV NHV/P68 (experiment 1).

<p>ASFV multiplex fluorescent microbead-based immunoassay (FMIA) sample-to-positive (S/P) serum antibody response (mean, SE) against three recombinant antigens (p30, p54, p72) in 9 pigs inoculated with ASFV NHV/P68 (experiment 1).</p

African swine fever virus (ASFV) antigen-specific serum antibody responses detected by multiplex fluorescent microbead-based immunoassay (FMIA) in pigs inoculated with replicon particle (RP) vaccines expressing p30, p54, and/or p72 genes.

<p>African swine fever virus (ASFV) antigen-specific serum antibody responses detected by multiplex fluorescent microbead-based immunoassay (FMIA) in pigs inoculated with replicon particle (RP) vaccines expressing p30, p54, and/or p72 genes.</p

ASFV recombinant p30 antibody ELISA serum-to-positive (S/P) responses in serum (mean, SE) and oral fluid (mean, SE) samples from 17 pigs (experiments 1 and 2) inoculated with ASFV NHV/P68.

<p>ASFV recombinant p30 antibody ELISA serum-to-positive (S/P) responses in serum (mean, SE) and oral fluid (mean, SE) samples from 17 pigs (experiments 1 and 2) inoculated with ASFV NHV/P68.</p