Table_1_C2H2 Zinc Finger Proteins: Master Regulators of Abiotic Stress Responses in Plants.docx

Abiotic stresses such as drought and salinity are major environmental factors that limit crop yields. Unraveling the molecular mechanisms underlying abiotic stress resistance is crucial for improving crop performance and increasing productivity under adverse environmental conditions. Zinc finger proteins, comprising one of the largest transcription factor families, are known for their finger-like structure and their ability to bind Zn2+. Zinc finger proteins are categorized into nine subfamilies based on their conserved Cys and His motifs, including the Cys2/His2-type (C2H2), C3H, C3HC4, C2HC5, C4HC3, C2HC, C4, C6, and C8 subfamilies. Over the past two decades, much progress has been made in understanding the roles of C2H2 zinc finger proteins in plant growth, development, and stress signal transduction. In this review, we focus on recent progress in elucidating the structures, functions, and classifications of plant C2H2 zinc finger proteins and their roles in abiotic stress responses.</p

Additional file 2: of Clinical and genetic characteristics of Chinese patients with familial or sporadic pediatric cataract

Table S2. The primers for haplotype analysis and allele specific PCR. (XLS 25 kb

Additional file 4: of Clinical and genetic characteristics of Chinese patients with familial or sporadic pediatric cataract

Table S4. Pediatric cataract families with uncertain significance variants. (XLS 29 kb

Additional file 5: of Clinical and genetic characteristics of Chinese patients with familial or sporadic pediatric cataract

Figure S1. Pedigrees of the families without suspected causative variants. (TIF 8488 kb

Additional file 3: of Clinical and genetic characteristics of Chinese patients with familial or sporadic pediatric cataract

Table S3. Target NGS analysis. (XLS 28 kb

Additional file 1: of Clinical and genetic characteristics of Chinese patients with familial or sporadic pediatric cataract

Table S1. List of 80 pediatric cataract genes. (XLS 36 kb

MOESM5 of Molecular analysis of inherited cardiomyopathy using next generation semiconductor sequencing technologies

Additional file 5: Table S4. Variants of uncertain significance or benign identified by cardiomyopathy NGS panels in this study

MOESM3 of Molecular analysis of inherited cardiomyopathy using next generation semiconductor sequencing technologies

Additional file 3: Table S2. List of variant annotation information

MOESM4 of Molecular analysis of inherited cardiomyopathy using next generation semiconductor sequencing technologies

Additional file 4: Table S3. Clinical characteristics of the patients with positive variants

Multiple SNPs Detection Based on Lateral Flow Assay for Phenylketonuria Diagnostic

Single nucleotide polymorphisms (SNPs) are closely related to genetic diseases, but current SNP detection methods, such as DNA microarrays that include tedious procedures and expensive, sophisticated instruments, are unable to perform rapid SNPs detection in clinical practice, especially for those multiple SNPs related to genetic diseases. In this study, we report a sensitive, low cost, and easy-to-use point-of-care testing (POCT) system formed by combining amplification refractory mutation system (ARMS) polymerase chain reaction with gold magnetic nanoparticles (GMNPs) and lateral flow assay (LFA) noted as the ARMS-LFA system, which allow us to use a uniform condition for multiple SNPs detection simultaneously. The genotyping results can be explained by a magnetic reader automatically or through visual interpretation according to the captured GMNPs probes on the test and control lines of the LFA device. The high sensitivity (the detection limit of 0.04 pg/μL with plasmid) and specificity of this testing system were found through genotyping seven pathogenic SNPs in phenylalanine hydroxylase gene (<i>PAH</i>, the etiological factor of phenylketonuria). This system can also be applied in DNA quantification with a linear range from 0.02 to 2 pg/μL of plasmid. Furthermore, this ARMS-LFA system was applied to clinical trials for screening the seven pathogenic SNPs in <i>PAH</i> of 23 families including 69 individuals. The concordance rate of the genotyping results detected by the ARMS-LFA system was up to 97.8% compared with the DNA sequencing results. This method is a very promising POCT in the detection of multiple SNPs caused by genetic diseases