Maquetas Taller vertical 2014-2: Granjas urbanas

<p>Normal mice (without <i>H</i>. <i>pylori</i> infection) were assigned as a control group (CG group) and mice models infected with <i>H</i>. <i>pylori</i> were divided into model group (MG group, only treated with saline solution), triple combination therapy group (TG group, the daily medicine intake is 0.5 ug clarithromycin, 0.02 ug omeprazole, and 1 ug amoxicillin), low/middle/high concentrations of flavonoid glycoside group (LF/MF/HF group, treated with one daily dose of flavonoid glycoside at 32/64/128 ug), low/middle/high concentrations of flavonoid glycoside and common concentration of amoxicillin group (LFA/MFA/HFA group, treated with one daily dose of Flavonoid glycoside at 32/64/128 ug and amoxicillin at 1 ug). Analysis of G cells and somatostatin gray value in each group (n = 10). *<i>P</i> < 0.05 vs model group.</p

Analysis of <i>H</i>. <i>pylori</i>clearance between model group and other treatment groups.

<p>Note:</p><p>^aThe number is determined by the relative amounts of <i>H</i>. <i>pylori</i>-specific CagA DNA.</p><p>*<i>P<0</i>.<i>05</i> vs model group (n = 8).</p><p>Analysis of <i>H</i>. <i>pylori</i>clearance between model group and other treatment groups.</p

The comparision for histochemistry and histopathology between a control and a model group.

<p>Giemsa staining (×400), Giemsa stain was used to observe the adherence of pathogenic bacteria to gastric cells. Microaerophilic culturing of <i>H</i>. <i>pylori</i>, the extracellular <i>H</i>. <i>pylori</i> are microaerophilic. Hematoxylin and Eosin staining of gastric tissues was used for the detection of <i>H</i>. <i>pylori</i> (HE×200). The staining of G cells of Gastric mucosa-associated lymphoid tissue (MALT) lymphoma (PV×200). The staining of D cells of Gastric MALT lymphoma (PV×200).</p

Hematoxylin and Eosin staining of gastric tissues (HE×200).

<p>Normal mice (without <i>H</i>. <i>pylori</i> infection) were assigned as a control group (CG group) and mice models infected with <i>H</i>. <i>pylori</i> were divided into model group (MG group, only treated with saline solution), triple combination therapy group (TG group, the daily medicine intake is 0.5 ug clarithromycin, 0.02 ug omeprazole, and 1 ug amoxicillin), low/middle/high concentrations of flavonoid glycoside group (LF/MF/HF group, treated with one daily dose of Flavonoid glycoside at 32/64/128 ug), low/middle/high concentrations of flavonoid glycoside and common concentration of amoxicillin group (LFA/MFA/HFA group, treated with one daily dose of flavonoid glycoside at 32/64/128 ug and amoxicillin at 1 ug). Pathological score of mice gastric antrum in each group (±SD, n = 10). *<i>P</i> < 0.05 vs model group.</p

The mRNA levels of Gastrin in the gastric mucosa of <i>H</i>. <i>pylori</i> infected mice in different groups (n = 6).

<p>*<i>P</i> < 0.05 vs model group</p><p>The mRNA levels of Gastrin in the gastric mucosa of <i>H</i>. <i>pylori</i> infected mice in different groups (n = 6).</p

The flowchart of study.

<p>Normal mice (without <i>H</i>. <i>pylori</i> infection) were assigned as a control group (CG group) and mice models infected with <i>H</i>. <i>pylori</i> were divided into model group (MG group, only treated with saline solution), triple combination therapy group (TG group, the daily medicine intake is 0.5 ug clarithromycin, 0.02 ug omeprazole, and 1 ug amoxicillin), low/middle/high concentrations of flavonoid glycoside group (LF/MF/HF group, treated with one daily dose of flavonoid glycoside at 32/64/128 ug), low/middle/high concentrations of flavonoid glycoside and common concentration of amoxicillin group (LFA/MFA/HFA group, treated with one daily dose of Flavonoid glycoside at 32/64/128 ug and amoxicillin at 1 ug). Gastrin, GAS; Somatostatin, SS.</p

Sensitivity test of <i>H</i>. <i>pylori</i> to flavonoid glycoside.

<p>A: blank control. B: 40 ug/mL flavonoid glycoside. C: 80 ug/mL flavonoid glycoside.</p

The effects of flavonoid glycoside on gastric cells infected by <i>H</i>. <i>pylori</i>.

<p>A, the effects of different concentrations of flavonoid glycoside on the number of gastric cells MGC803. B, the effects of different concentrations of flavonoid glycoside on protein levels of INF-gamma. C, the effects of different concentrations of flavonoid glycoside on protein levels of IL-4. D, the effects of different concentrations of flavonoid glycoside on protein levels of gastrin. E, the effects of different concentrations of flavonoid glycoside on protein levels of somatostatin. *<i>P</i> < 0.05 vs a control group without flavonoid glycoside.</p

The mRNA levels of IL-4 in the gastric mucosa of <i>H</i>. <i>pylori</i> infected mice in different groups (<i>n</i> = 6).

<p>Note:</p><p>^▲<i>P</i> < 0.05 via a CG group,</p><p>*<i>P</i> < 0.05 via a MG group.</p><p>The mRNA levels of IL-4 in the gastric mucosa of <i>H</i>. <i>pylori</i> infected mice in different groups (<i>n</i> = 6).</p

The mRNA levels of IFN-gamma in the gastric mucosa of <i>H</i>. <i>pylori</i> infected mice in different groups (n = 6).

<p>^▲<i>P</i> < 0.05 model group vs blank group;</p><p>*<i>P</i> < 0.05 vs model group.</p><p>The mRNA levels of IFN-gamma in the gastric mucosa of <i>H</i>. <i>pylori</i> infected mice in different groups (n = 6).</p