Disease Burden of Mild Asthma: Findings from a Cross-Sectional Real-World Survey

<p><strong>Article full text</strong></p> <p><br> The full text of this article can be found <a href="https://link.springer.com/article/10.1007/s12325-017-0520-0"><b>here</b>.</a><br> <br> <strong>Provide enhanced digital features for this article</strong><br> If you are an author of this publication and would like to provide additional enhanced digital features for your article then please contact <u>[email protected]</u>.<br> <br> The journal offers a range of additional features designed to increase visibility and readership. All features will be thoroughly peer reviewed to ensure the content is of the highest scientific standard and all features are marked as ‘peer reviewed’ to ensure readers are aware that the content has been reviewed to the same level as the articles they are being presented alongside. Moreover, all sponsorship and disclosure information is included to provide complete transparency and adherence to good publication practices. This ensures that however the content is reached the reader has a full understanding of its origin. No fees are charged for hosting additional open access content.<br> <br> Other enhanced features include, but are not limited to:<br> • Slide decks<br> • Videos and animations<br> • Audio abstracts<br> • Audio slides<u></u></p

Video_1_v1_Laparo-Endoscopic Single Site Combined With Hysteroscopy to Diagnose and Treat Robert’s Uterine Malformation: A Case Report.mov

Asymmetric septate uterus, commonly known as Robert’s uterus, is an exceedingly rare uterine malformation described for the first time in 1970 by Robert H. Currently, surgery is the therapy of choice for Robert’s uterus, with surgical choices ranging from laparotomy to minimally invasive surgery. In this paper, we reported that a 14-year-old girl with primary dysmenorrhea that gradually worsened three months after menarche had surgery after many imaging evaluations, and that the intraoperative diagnosis was Robert’s uterus. The diagnostic and therapeutic laparo-endoscopic single site(LESS) combined with hysteroscopy surgery for Robert’s uterine abnormality was shown via a step-by-step presentation of the method accompanied by narrated video footage. During the ten-month postoperative follow-up period, the patient had monthly recurrences with normal menstrual volume and no dysmenorrhea, demonstrating that as a minimally invasive treatment, LESS combined with hysteroscopy surgery is a successful methodfor diagnosing and treating this specific malformation.</p

Characterizing Binding of Small Molecules. II. Evaluating the Potency of Small Molecules to Combat Resistance Based on Docking Structures

Drug resistance severely erodes the efficacy of therapeutic treatments for many diseases. Assessing the potency of a drug lead to combat resistance is no doubt critical for designing new drugs or new therapeutic combinations. Virtual screening is often the first step in drug discovery and a challenging problem is to accurately predict the resistant profile of an inhibitor based on the docking structures. Using a well studied system HIV-1 protease, we have illustrated the success of a computational method called MIEC-SVM on tackling this problem. We computed molecular interaction energy components (MIECs) between the ligand and the protease residues to characterize the docking poses, which were input to support vector machine (SVM) to distinguish resistant from nonresistant mutants. More importantly, the method is able to predict resistant profiles for new drugs based on the docking structures as indicated by its satisfactory performance in leave-one-drug-out and leave-drug/mutants-out tests. Therefore, the MIEC-SVM method can also facilitate designing effective therapeutic combinations by combining drugs with complementary resistant profiles

Characterization of Small Molecule Binding. I. Accurate Identification of Strong Inhibitors in Virtual Screening

Accurately ranking docking poses remains a great challenge in computer-aided drug design. In this study, we present an integrated approach called MIEC-SVM that combines structure modeling and statistical learning to characterize protein–ligand binding based on the complex structure generated from docking. Using the HIV-1 protease as a model system, we showed that MIEC-SVM can successfully rank the docking poses and consistently outperformed the state-of-art scoring functions when the true positives only account for 1% or 0.5% of all the compounds under consideration. More excitingly, we found that MIEC-SVM can achieve a significant enrichment in virtual screening even when trained on a set of known inhibitors as small as 50, especially when enhanced by a model average approach. Given these features of MIEC-SVM, we believe it provides a powerful tool for searching for and designing new drugs

Table1_Characterization of the Different Subtypes of Immune Cell Infiltration to Aid Immunotherapy.XLS

Background?PD-1 ablation or PD-L1 specific monoclonal antibody against PD-1 can recruit the accumulation of functional T cells, leading to tumor rejection in the microenvironment and significantly improving the prognosis of various cancers. Despite these unprecedented clinical successes, intervention remission rates remain low after treatment, rarely exceeding 40%. The observation of PD-1/L1 blocking in patients is undoubtedly multifactorial, but the infiltrating degree of CD8+T cell may be an important factor for immunotherapeutic resistance.Methods:We proposed two computational algorithms to reveal the immune cell infiltration (ICI) landscape of 1646 lung adenocarcinoma patients. Three immune cell infiltration patterns were defined and the relative ICI scoring depended on principal-component analysis.Results:A high ICI score was associated with the increased tumor mutation burden and cell proliferation-related signaling pathways. Different cellular signaling pathways were observed in low ICI score subtypes, indicating active cell proliferation, and may be associated with poor prognosis.Conclusion:Our research identified that the ICI scores worked as an effective immunotherapy index, which may provide promising therapeutic strategies on immune therapeutics for lung adenocarcinoma.</p

Phenotypic characterization of the <i>dj-lm</i> mutant.

<p><b>(A)</b> Representative leaves of Dongjin (DJ) and <i>dj-lm</i> plants. <b>(B)</b> Trypan blue staining of DJ and <i>dj-lm</i> leaves. <b>(C)</b> Diamiobenzidine (DAB) staining of DJ and <i>dj-lm</i> leaves. <b>(D)</b> DJ and <i>dj-lm</i> plants grown in the field. <b>(E)</b> Disease phenotypes of DJ and <i>dj-lm</i> after inoculation with <i>M</i>. <i>oryzae</i> isolate RO1-1. Similar results were obtained from three independent experiments. Bar = 1 cm. <b>(F)</b> Lesion length of DJ and <i>dj-lm</i> after inoculation with RO1-1. Values are means ± standard errors of 10 replications. Significance was determined at ***P<0.0001 with a Student’s <i>t</i>-test. <b>(G)</b> Relative fungal biomass of DJ and <i>dj-lm</i> after inoculation with <i>M</i>. <i>oryzae</i>. Values are means ± standard errors of 10 replications. Significance was determined at *P<0.05 with a Student’s <i>t</i>-test. <b>(H)</b> Disease phenotypes of DJ and <i>dj-lm</i> after inoculation with <i>Xoo</i> strain PXO-99. Similar results were obtained from three independent experiments. Bar = 1 cm. <b>(I)</b> Lesion length of DJ and <i>dj-lm</i> after inoculation with PXO99. Values are means ± standard errors of 10 replications. Significance was determined at ***P<0.0001 with a Student’s <i>t</i>-test.</p

Carrier Transport in Dye-Sensitized Solar Cells Using Single Crystalline TiO₂ Nanorods Grown by a Microwave-Assisted Hydrothermal Reaction

Single crystalline rutile nanorod was grown directly on top of fluorine-doped tin oxide (FTO) substrate via a microwave assisted hydrothermal reaction which dramatically increased a growth rate over a conventional hydrothermal method. In addition, the introduction of thin TiO2 seed layer to FTO substrates promotes heterogeneous nucleation and increases the density. Dye-sensitized solar cells (DSSCs) were fabricated using the rutile nanorods that were differently treated with TiCl4 solution and the carrier transport mechanism in the nanorod-based DSSCs was systematically examined. When the nanorods were treated with TiCl4, more dye was adsorbed on the TiO2 films and the energy conversion efficiency increased to 3.7% for a 2.5 μm thick TiO2 film. Stepped light induced-transient measurement of photocurrent and voltage measurements showed that the role of the nanorods in DSSCs is to increase an electron diffusion coefficient in TiO2 mesoporous films. In contrast to the diffusion coefficient, the lifetime of electron is not dependent on the presence of the nanorods. To explain the experimental observations, we propose a surface diffusion model for electrons that are injected into the rutile nanorods from dye molecules. This surface diffusion may originate from the high crystallinity of nanorods and the homogeneous contact between nanorod and coated nanoparticle layer

The Rice Dynamin-Related Protein OsDRP1E Negatively Regulates Programmed Cell Death by Controlling the Release of Cytochrome <i>c</i> from Mitochondria

<div><p>Programmed cell death (PCD) mediated by mitochondrial processes has emerged as an important mechanism for plant development and responses to abiotic and biotic stresses. However, the role of translocation of cytochrome <i>c</i> from the mitochondria to the cytosol during PCD remains unclear. Here, we demonstrate that the rice dynamin-related protein 1E (OsDRP1E) negatively regulates PCD by controlling mitochondrial structure and cytochrome <i>c</i> release. We used a map-based cloning strategy to isolate <i>OsDRP1E</i> from the lesion mimic mutant <i>dj-lm</i> and confirmed that the E409V mutation in OsDRP1E causes spontaneous cell death in rice. Pathogen inoculation showed that <i>dj-lm</i> significantly enhances resistance to fungal and bacterial pathogens. Functional analysis of the E409V mutation showed that the mutant protein impairs OsDRP1E self-association and formation of a higher-order complex; this in turn reduces the GTPase activity of OsDRP1E. Furthermore, confocal microscopy showed that the E409V mutation impairs localization of OsDRP1E to the mitochondria. The E409V mutation significantly affects the morphogenesis of cristae in mitochondria and causes the abnormal release of cytochrome <i>c</i> from mitochondria into cytoplasm. Taken together, our results demonstrate that the mitochondria-localized protein OsDRP1E functions as a negative regulator of cytochrome <i>c</i> release and PCD in plants.</p></div

Carbon-Encapsulated F‑Doped Li₄Ti₅O₁₂ as a High Rate Anode Material for Li⁺ Batteries

TiO₂ nanoparticles aggregated into a regular ball-in-ball morphology were synthesized by hydrothermal processing and converted to carbon-encapsulated F-doped Li₄Ti₅O₁₂ (LTO) composites (C-FLTO) by solid state lithiation at high temperatures. Through the careful control of the amount of carbon precursor (d(+)-glucose monohydrate) used in the process, LTO encapsulated with a continuous layer of nanoscale carbon was formed. The carbon encapsulation served a dual function: preserving the ball-in-ball morphology during the transformation from TiO₂ to LTO and decreasing the external electron transport resistance. The fluoride doping of LTO not only increased the electron conductivity of LTO through trivalent titanium (Ti³⁺) generation, but also increased the robustness of the structure to repeated lithiation and delithiation. The best-performing composite, C-FLTO-2, therefore delivered a very satisfying performance for a LTO anode: a high charge capacity of ∼158 mA h g^–1 at the 1 C rate with negligible capacity fading for 200 cycles and an extremely high rate performance up to 140 C

Subcellular localization of OsDRP1E-GFP and E409V-GFP <i>in planta</i>.

<p>(A) Confocal images of OsDRP1E-GFP and E409V-GFP transiently expressed in rice protoplasts. MitoTracker was used as the mitochondrial marker. Bar = 10 μm. (B) Confocal images of OsDRP1E-GFP and E409V-GFP transiently expressed in <i>N</i>. <i>benthamiana</i>. Ds-RED-tagged COX4 was used as the mitochondrial marker. Bar = 10 μm.</p