4 research outputs found
In vitro evaluation of fungal endophytes of black pepper against Phytophthora capsici and Radopholus similis
Experiment on in vitro screening of 125 endophytic fungi of black pepper against Phytophthora capsici indicated that 23 isolates showed more than 50% inhibition. The nematicidal activity of metabolites from endophytic fungi was also tested on Radopholus similis and the isolate BPEF73 (Daldinia eschscholtzii) showed highest mortality up to 60%. The isolates showing biocontrol potential were characterized based on internal transcribed spacer (ITS1 and ITS2) regions of rDNA. The sequence analysis of the isolates showed maximum identity with Annulohypoxylon nitens (BPEF25 and BPEF38), Daldinia eschscholtzii (BPEF41 and BPEF73), Fusarium spp. (BPEF72 and BPEF75), Ceriporia lacerata (BPEF81), Diaporthe sp. (BPEF11) and Phomopsis sp. (BPEF83). This is first report of endophytic association of above fungi in black pepper and the exploitation of their biocontrol potential against the major black pepper pathogens P. capsici and R. similis.
 
In vitro evaluation of fungal endophytes of black pepper against Phytophthora capsici and Radopholus similis
Experiment on in vitro screening of 125 endophytic fungi of black pepper against Phytophthora capsici indicated that 23 isolates showed more than 50% inhibition. The nematicidal activity of metabolites from endophytic fungi was also tested on Radopholus similis and the isolate BPEF73 (Daldinia eschscholtzii) showed highest mortality up to 60%. The isolates showing biocontrol potential were characterized based on internal transcribed spacer (ITS1 and ITS2) regions of rDNA. The sequence analysis of the isolates showed maximum identity with Annulohypoxylon nitens (BPEF25 and BPEF38), Daldinia eschscholtzii (BPEF41 and BPEF73), Fusarium spp. (BPEF72 and BPEF75), Ceriporia lacerata (BPEF81), Diaporthe sp. (BPEF11) and Phomopsis sp. (BPEF83). This is first report of endophytic association of above fungi in black pepper and the exploitation of their biocontrol potential against the major black pepper pathogens P. capsici and R. similis.
 
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Not AvailableExperiment on in vitro screening of 125 endophytic fungi of black pepper against Phytophthora capsici indicated that 23 isolates showed more than 50% inhibition. The nematicidal activity of metabolites from endophytic fungi was also tested on Radopholus similis and the isolate BPEF73 (Daldinia eschscholtzii) showed highest mortality up to 60%. The isolates showing biocontrol potential were characterized based on internal transcribed spacer (ITS1 and ITS2) regions of rDNA. The sequence analysis of the isolates showed maximum identity with Annulohupoxylon nitens (BPEF25 and BPEF38), Daldinia eschscholtzii (BPEF41 and BPEF73), Fusarium spp. (BPEF72 and BPEF75), Ceriporia lacerata (BPEF81), Diaporthe sp. (BPEF11) and Phomopsis sp. (BPEF83). This is first report of endophytic association of above fungi in black pepper and the exploitation of their biocontrol potential against the major black pepper pathogens P. capsici and R. similis.Not Availabl
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Not AvailableBlack pepper (Piper nigrum L.) plants (variety Sreekara) were produced through somatic embryogenesis and studied for endophytic fungal association and the role endophytic fungi in hardening tissues and its growth promotion. Interestingly, somatic embryo derived black pepper
plants are found to be free from fungal association. No Fungus could be obtained from the tissues of somatic derived black pepper plants. The colonization studies on somatic plants by endophytic fungi showed that stem and root tissues were successfully colonized by the inoculated endophytic fungi such as Diaporthe sp., Annulohypoxylon nitens, Daldinia eschscholtzii, Fusarium oxysporum and, Phomopsis sp. The colonization of black pepper tissues by the endophytic fungi were confirmed by re- isolation from the treated plants. Endophyte enriched sterile sand is a promising medium for the hardening of somatic derived black pepper plants with enhanced survival rate of 56% -69% when compared to control (50%). Application of endophytic fungi in black pepper showed a significant increase in growth parameters like plant height, number of leaves, number of nodes, fresh and dry weight of plant in under green house. This is the first report of using endophytic fungi for hardening tissue cultured plants of black pepperNot Availabl