Liu et al. - LiveImaging_data_structure

LiveImaging_data_structure.mat contains a data structure named "FlyLines" with Bicoid-GFP intensity gradient and cephalic furrow (CF) position measurements of fly embryos from live two-photon imaging for 29 fly lines

Liu et al. - BcdGFP_embryos_raw_data

High-resolution images of 28 embryos acquired by live two-photon microscopy; dorsal view, coronal plane (linear pixel dimension 0.46 micrometer). Embryos express Bcd-GFP fusion protein in a bcdE1 null mutant background (flyline 2XA). For 23 embryos two time points are provided: 16+/-2 min after mitosis 13 (for Bicoid gradient measurement) and 67+/-2 min after mitosis 13 (for cephalic furrow position measurement). Two additional embryos are provided for early time point (09 and 12); three additional embryos are provided for late time point (15, 24 and 28). Embryo 05 is missing from dataset. This dataset reproduces gradients in Figure 1C of main text

Liu et al. - Immunofluorescence_data_structure

ImmunoFluorescence_data_structure.mat contains a data structure named "Sessions" that contains raw gap ( Hb, Kr, Gt and Kni) and pair rule (Eve) gene expression (protein) intensity profiles measured using immunofluorescence imaging for fly embryos during n.c. 14 for 6 fly lines from our Bicoid-GFP fly line library