MOESM7 of Boosting LPMO-driven lignocellulose degradation by polyphenol oxidase-activated lignin building blocks

Additional file 7: Table S2. Selected cellulase-rich Ascomycota from the JGI database1

MOESM1 of Boosting LPMO-driven lignocellulose degradation by polyphenol oxidase-activated lignin building blocks

Additional file 1: Figure S1. Activity of MtLPMO9B towards amorphous cellulose in the presence and absence of MtPPO7 or AbPPO. HPAEC elution pattern of regenerated amorphous cellulose (RAC; 1.5 mg mL−1) incubated with MtLPMO9B (red, 5.0 μg mL−1) only, or with either AbPPO (blue, 2.5 µL mL−1) or MtPPO7 (yellow, 5.0 μg mL−1) in the presence of (a) para-coumaric acid (no. 3 specified in Table 1, 2 mM) and (b) 3-hydroxy-4-methoxycinnamic acid (no. 5 specified in Table 1, 2 mM). The incubation of RAC with MtLPMO9B results in the formation of non-oxidized gluco-oligosaccharides (GlcOSn) and C1-oxidized gluco-oligosaccharides (GlcOS n # ). See “Methods” for details

Additional file 1 of Unearthing the transition rates between photoreceptor conformers

Appendix. Appendix contains: the mathematical details to calculate the percentage of active photoreceptor within the population; calculating different forms of steady-state P A percentages; how the P A percentage is related to the scalar values X 1 and X 2 used in the Verméglio method; how the approach could be applied to simpler and more complex photoreceptor systems; using Butler’s model; pseudo-code for the optimisations algorithms used; experimental methods; Supplementary Figures and Tables. (PDF 290 kb

MOESM2 of Boosting LPMO-driven lignocellulose degradation by polyphenol oxidase-activated lignin building blocks

Additional file 2: Figure S2. Release of oligosaccharides from RAC incubated with MtLPMO9B in the presence and absence of MtPPO7 throughout 24 h. Samples were incubated in the presence of ferulic acid (no. 8 specified in Table 1). The total sum is shown as integrated peak areas of released non-oxidized (shaded red and shaded yellow) and C1-oxidized (red and yellow) gluco-oligosaccharides after incubation of regenerated amorphous cellulose (RAC; 1.5 mg mL−1) with MtLPMO9B only (red bars, 5 mg mL−1) and MtLPMO9B together with MtPPO7 (yellow bars, 5 mg mL−1) based on HPAEC. All incubations were performed in duplicate, and the standard deviations are presented as error bars. See “Methods” for details

MOESM7 of Boosting LPMO-driven lignocellulose degradation by polyphenol oxidase-activated lignin building blocks

Additional file 7: Table S2. Selected cellulase-rich Ascomycota from the JGI database1