Structural diversity of ovatoxins in Ostreopsis cf. ovata AZ strains and their impact on monitoring

No abstracts are to be cited without prior reference to the author. The structural diversity of ovatoxins (OvTXs) was studied in strains of Ostreopsis cf. ovata isolated from the Gulf of Naples (AZ strains) and grown in culture at Statione Zoologica A. Dohrn. ESILCTOF analysis, with complementary use of positive and negative ion modes revealed the occurrence of twelve palytoxin (PlTX) congeners. Five of them could be OvTX-a, OvTX-b, OvTX-c and OvTX-d/e, known in the literature but with undefined structures except for OvTX-a. Other congeners were indicated to be new having structural variations at the N-containing terminal units connected to the C1-carbon and/or at 115-NH2. The congeners seemed to share a similar carbon-chain backbone, a feature that should be taken into account when applying immunoassays for monitoring. The variances of the terminal units combined with the elusive LC behavior of some analogs should be taken into account when applying LC-MS/MS for determination. The occurrence of other unknown PlTX analogs was also detected, suggesting the need for further studies

Figure S7 from Comparative Transcriptome Profiling Reveals Coding and Noncoding RNA Differences in NSCLC from African Americans and European Americans

Hypergeometric testing for overlap between coding and noncoding expression signatures in AAs and EAs.</p

Figure S3 from Comparative Transcriptome Profiling Reveals Coding and Noncoding RNA Differences in NSCLC from African Americans and European Americans

Cross-comparison of race-enriched differential gene expression.</p

Figure S5 from Comparative Transcriptome Profiling Reveals Coding and Noncoding RNA Differences in NSCLC from African Americans and European Americans

Comparison of immune cell composition in lung tumors from AAs and EAs.</p

Relative abundance of NGS V4 Leptocylindraceae sequences at the six stations.

<p>Data for plankton samples were inferred from the surface cDNA results normalised over the total number of sequences obtained for the sample and the average of the three size fractions (mean relative frequency). Data for sediment samples were inferred from cDNA template based sequences (relative frequency).</p

Figure S4 from Comparative Transcriptome Profiling Reveals Coding and Noncoding RNA Differences in NSCLC from African Americans and European Americans

Unique pathway enrichment in lung tumors from AAs and EAs.</p

Figure S1 from Comparative Transcriptome Profiling Reveals Coding and Noncoding RNA Differences in NSCLC from African Americans and European Americans

Kaplan-Meier curves showing lung cancer-specific survival in AAs and EAs in the mRNA and miRNA cohorts.</p

Figure S2 from Comparative Transcriptome Profiling Reveals Coding and Noncoding RNA Differences in NSCLC from African Americans and European Americans

Unsupervised PCA of total gene expression from lung cancers in both AAs and EAs.</p

Figure S6 from Comparative Transcriptome Profiling Reveals Coding and Noncoding RNA Differences in NSCLC from African Americans and European Americans

Differential gene expression in AA tissues due to population stratification.</p

Table S1-S9 from Comparative Transcriptome Profiling Reveals Coding and Noncoding RNA Differences in NSCLC from African Americans and European Americans

Table S1 C2 pathway list. Table S2 C5 pathway list. Table S3 C6 pathway list. Table S4 cMap result list in AAs. Table S5 cMap result list in EAs. Table S6 Drug resistance and sensitivity comparison. Table S7 Population stratified genes in AAs. Table S8 Differentially expressed microRNAs in AAs and EAs. Table S9 Population stratified microRNAs in AAs.</p