Venn diagram showing shared and non-shared bacterial species between the hypobranchial gland and foot of <i>Dicathais orbita</i>.

<p>The number of species that have biosynthetic capabilities relevant to Tyrian purple production are highlighted in different colours (Orange = indole producers; Blue = brominating enzymes; Purple = indole producers and brominating capabilities).</p

Phylogenetic tree of <i>Dicathais orbita</i> samples generated from 16S rRNA sequences by MEGAN.

<p>A = Female hypobranchial gland (F2H); B = Male hypobranchial gland (M1H); C = Female foot (F3F); D = Male foot (M3F). All these sample types have more than 15,000 reads.</p

Additional file 1: Figure S1. of Genome and transcriptome sequencing characterises the gene space of Macadamia integrifolia (Proteaceae)

Kmer coverage plot for optimized kmer of 26 used to estimate haploid genome size of 652 Mb (600–700 Mb). Coloured curves correspond to the complete statistical model including erroneous and genomic kmers (red), using a diploid model, heterozygous kmers with major peak at 14 (green) and homozygous kmers with subpeak at 7 (blue). (PDF 5 kb

Additional file 3: Figure S3. of Genome and transcriptome sequencing characterises the gene space of Macadamia integrifolia (Proteaceae)

RNA-seq read mapping to candidate genes for cyanogenesis in macadamia, including those encoding the cytochrome P450s CYP79 and CYP71, glycosyltransferase UGT85 and β-glucosidase. (PDF 535 kb

Alpha diversity showing the richness of bacterial community diversity within <i>Dicathais orbita</i> foot (F2F, F3F, M2F and M3F) and hypobranchial gland samples (F1H, F2H, M1H and M2H) (F = female; M = male).

<p>The phylogenetic diversity metric consists of genus richness based on 3585 observed OTUs at the 97% sequence similarity level and 443 possible observed genus. Sample with reads of more than 3000 are visible.</p

Similarity of percentages (SIMPER) analysis showing the bacterial genus that contribute most to the differences between hypobranchial gland and foot of <i>Dicathais orbita</i> (Average dissimilarity = 68.51).

<p>Similarity of percentages (SIMPER) analysis showing the bacterial genus that contribute most to the differences between hypobranchial gland and foot of <i>Dicathais orbita</i> (Average dissimilarity = 68.51).</p

Principal Coordinates Ordination (PCO) of bacterial genus composition, based on a Bray Curtis similarity matrix of the relative abundance of OTUs at 97% sequence similarity level for the hypobranchial gland (purple) and foot (orange) of female (F) and male (M) <i>Dicathais orbita</i>.

<p>Principal Coordinates Ordination (PCO) of bacterial genus composition, based on a Bray Curtis similarity matrix of the relative abundance of OTUs at 97% sequence similarity level for the hypobranchial gland (purple) and foot (orange) of female (F) and male (M) <i>Dicathais orbita</i>.</p

Mean (+s.e.) number of OTUs in the hypobranchial gland and foot tissue of <i>Dicathais orbita</i>, showing the mean proportion unique to individuals samples of foot and hypobranchial gland tissue.

<p>(A) = OTUs richness, (B) = H index/diversity.</p

Summary of <i>Dicathais orbita</i> hypobranchial gland and foot tissue 16S rRNA bacterial profiling.

<p>¹ The samples are labelled such that the first letter refers to the gender, the number to different replicate snails within each gender and the second letter to the tissue type.</p><p>² OTUs are shared among multiple samples and are based on 97% sequence similarity criteria in the Silva_119 database.</p><p>Summary of <i>Dicathais orbita</i> hypobranchial gland and foot tissue 16S rRNA bacterial profiling.</p