7 research outputs found

    Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, -3

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    Tellites selected from each LG for screening the BAC library. Markers in italic (initiated with LcaB) represent microsatellites isolated from BAC clones and newly mapped to the map.<p><b>Copyright information:</b></p><p>Taken from "Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, "</p><p>http://www.biomedcentral.com/1471-2164/9/139</p><p>BMC Genomics 2008;9():139-139.</p><p>Published online 25 Mar 2008</p><p>PMCID:PMC2329641.</p><p></p

    Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, -1

    No full text
    D grouped. Results indicate that the average insert size is 98 kb with over 80% of the clones > 80 kb.<p><b>Copyright information:</b></p><p>Taken from "Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, "</p><p>http://www.biomedcentral.com/1471-2164/9/139</p><p>BMC Genomics 2008;9():139-139.</p><p>Published online 25 Mar 2008</p><p>PMCID:PMC2329641.</p><p></p

    Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, -0

    No full text
    h under the following conditions: ramp pulse time of 5–15 s at 6 V/cm, temperature at 14°C. Markers used are Lambda Ladder PFG Marker (outside lanes) and MidRange II PFG Marker (NEB, SG, Singapore). The 8 kb common band is the pCC1BAC Vector (Epicentre, WI, USA).<p><b>Copyright information:</b></p><p>Taken from "Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, "</p><p>http://www.biomedcentral.com/1471-2164/9/139</p><p>BMC Genomics 2008;9():139-139.</p><p>Published online 25 Mar 2008</p><p>PMCID:PMC2329641.</p><p></p

    Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, -4

    No full text
    <p><b>Copyright information:</b></p><p>Taken from "Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, "</p><p>http://www.biomedcentral.com/1471-2164/9/139</p><p>BMC Genomics 2008;9():139-139.</p><p>Published online 25 Mar 2008</p><p>PMCID:PMC2329641.</p><p></p

    Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, -2

    No full text
    Ll microtiter plates. Lanes 1–11: superpools 1–11 and lane 12: genomic DNA as positive control. Each superpool contains DNA of 12 plates or 4,608 individual BAC clones. In five superpools (3, 4, 8, 9 and 10), PCR product was amplified by the marker Lca064. Second round PCR screening in 48 pools from the superpool number 3. Three pools (6, 35 and 41) showed a signal amplified by the marker Lca064. Third round PCR screening in a 96-well plate from the pool number 6. Three positive clones (26, 29 and 86) were detected in the plate by the marker Lca064.<p><b>Copyright information:</b></p><p>Taken from "Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, "</p><p>http://www.biomedcentral.com/1471-2164/9/139</p><p>BMC Genomics 2008;9():139-139.</p><p>Published online 25 Mar 2008</p><p>PMCID:PMC2329641.</p><p></p

    Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, -6

    No full text
    <p><b>Copyright information:</b></p><p>Taken from "Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, "</p><p>http://www.biomedcentral.com/1471-2164/9/139</p><p>BMC Genomics 2008;9():139-139.</p><p>Published online 25 Mar 2008</p><p>PMCID:PMC2329641.</p><p></p
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