13 research outputs found

    Histological analysis of liver tissue following intraportal administration of BM-MSC after MCD diet and PHx in rat.

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    <p>Haematoxylin-eosin (H–E) staining (upper panel), Oil Red O staining (middle panel) and PAS/OX-1 staining (lower panel). (i) after 13 weeks of control diet (GROUP A), (ii) after 4 weeks of MCD diet, followed by PHx and control diet for 9 weeks (GROUP B), (iii) after 4 weeks of MCD diet, followed by PHx, BM-MSC administration and control diet for 9 weeks (GROUP C). Representative images were chosen out of 3 mice analysed per experimental group. For H-E staining, scale bars in the main image indicate 500 μm, while scale bars in the inset images indicate 50 μm. For Oil Red O and PAS/OX-1 staining, scale bars indicate 100 μm.</p

    Histological analysis of liver tissue following PHx and/or MCD.

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    <p>Haematoxylin-eosin (H–E) staining (upper panel), Oil Red O staining (middle panel) and PAS/OX-1 staining (lower panel). (i) after 4 weeks of control diet (GROUP A*), (ii) after 4 weeks of MCD diet (GROUP C*), (iii) after 13 weeks of control diet (GROUP A), (iv) after 13 weeks of control diet with PHx at week 4 (GROUP B), (v) after 4 weeks of MCD diet followed by 9 weeks of control diet (GROUP C), (vi) after 4 weeks of MCD diet followed by PHx and 9 weeks of control diet (GROUP D), (vii) after 13 weeks of MCD diet (GROUP E), (viii) after 13 weeks of MCD diet with PHx at week 4 (GROUP F). Representative images were chosen out of 3 mice analysed per experimental group. For H-E staining, scale bars in the main image indicate 500 μm, while scale bars in the inset images indicate 50 μm. For Oil Red O and PAS/OX-1 staining, scale bars indicate 100 μm.</p

    Recovery assessment following partial hepatectomy (PHx) and/or methionine/choline-deficient (MCD) diet in rat.

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    <p>(A) Study design. (B) Mean liver weight for each experimental group at week 4 before PHx (left panel), at week 4 directly after PHx (middle panel) and at week 13 (end of study, right panel). (C) Evolution of body weight for each experimental group. Data are expressed in gram ± standard error. (D) Evolution of total serum bilirubin (TSB) level for each experimental group. Data are expressed in milligram per decilitre ± standard error. (E) Evolution of serum triglyceride (TG) level for each experimental group. Data are expressed in milligram per decilitre ± standard error. (F) Evolution of serum cholinesterase (CHE) level for each experimental group. Data are expressed in units per litre ± standard error. For each experimental group at each time point n=10.</p

    <i>In</i><i>vivo</i> / <i>ex</i><i>vivo</i> bioluminescence imaging (BLI) following intravenous (IV) and intraportal (IP) administration of BM-MSC/eGFP-Luc.

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    <p>(A) Bright field phase contrast microscopic image of cultured rat BM-MSC. The scale bar indicates 100 μm. RT-PCR analysis of cultured BM-MSC: GAPDH, vimentin, AFP, albumin, CD45. A representative output was chosen from two independent measurements at multiple passages. Flow cytometric overlay histograms showing the expression pattern of membrane proteins on BM-MSC derived from Lewis rats (i.e. expression of CD27, CD73 and CD90.1, but no expression of CD45). Open histograms: unstained control. Filled histograms: specific antibody staining. A representative histogram overlay was chosen from three independent measurements at multiple passages. (B) Overlay histogram: level of eGFP expression by control BM-MSC (open black histogram) and transduced BM-MSC/eGFP-Luc (filled green histogram). A representative histogram overlay was chosen from four independent measurements at multiple passages. Right graph: In vitro luminescence assay using 1x10<sup>5</sup> control BM-MSC and transduced BM-MSC/eGFP-Luc. Data are expressed as mean relative light units ± standard error (n=4 measurements at multiple passages). (C) Representative BLI images of healthy (upper panel) and MCD diet-fed animals (lower panel) subjected to PHx at week 4 with intravenous BM-MSC/eGFP-Luc administration at week 5 (n=2). (D) Representative BLI images of control animals (upper panel) with intraportal BM-MSC/eGFP-Luc administration (n=4) and BLI images of healthy (middle panel) and MCD diet-fed animals (lower panel) subjected to PHx at week 4 with intraportal BM-MSC/eGFP-Luc administration at week 5 (n=3). Left panel: <i>In vivo</i> BLI (left picture) and <i>ex vivo</i> BLI (right picture) at 2 hours post-implantation. Right panel: <i>In vivo</i> BLI (left picture) and <i>ex vivo</i> BLI (right picture) at 24 hours post-implantation. <i>Ex vivo</i> images show the following organs in clockwise direction: lung (upper), heart, spleen, kidney, liver.</p

    Recovery assessment following intraportal administration of BM-MSC after MCD diet and PHx in rat.

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    <p>(A) Study design. (B) Mean liver weight for each experimental group at week 4 before PHx (left panel), at week 4 directly after PHx (middle panel) and at week 13 (end of study, right panel). (C) Evolution of body weight for each experimental group. Data are expressed in in gram ± standard error. (D) Evolution of total serum bilirubin (TSB) level for each experimental group. Data are expressed in milligram per decilitre ± standard error. (E) Evolution of serum triglyceride (TG) level for each experimental group. Data are expressed in milligram per decilitre ± standard error. (F) Evolution of serum cholinesterase (CHE) level for each experimental group. Data are expressed in units per litre ± standard error. For each experimental group at each time point n=10.</p

    A representative example of an ocular surface photograph within the image processing software.

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    <p>(a) The solid blue line demarcates the limbus from the vascular cornea. In (b) the output image is illustrated with the large vessels in red, medium sized vessels in green and small vessels in blue. In (c) the calculated percentage area covered by each vessel type is depicted.</p

    Cytokine concentrations in corneal epithelial secretions from normal and vascularized eyes.

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    <p>Cytokine levels of tears collected with a cornea bath (100× diluted) were measured for the Normal Control group (NC, n = 20), Patient’s Control group (PC, n = 8) and Patients Affected group (PA, n = 16). Horizontal lines represent mean Concentrations with SEM. Differences are significant if **p<0.001.</p

    Correlations of % vascularization and levels of cytokines in corneal tear samples collected using a corneal bath.

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    <p>The % area vascularization was calculated as the sum of small, medium and large vessels and compared to the cytokine levels in corneal epithelial secretions. For these experiments, 12 cornea’s of 9 patients were included. Correlations are statistically significant if p<0.05 and the dotted lines indicate the 95% confidence intervals.</p
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